Biology:Revival of the woolly mammoth

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Short description: Efforts to revive the woolly mammoth
Model of a woolly mammoth at the Royal BC Museum

The revival of the woolly mammoth is a proposed hypothetical that frozen soft-tissue remains and DNA from extinct woolly mammoths could be a means of regenerating the species. Several methods have been proposed to achieve this goal, including cloning, artificial insemination, and genome editing. Whether or not it is ethical to create a live mammoth is debated.

In 2003, the Pyrenean ibex was briefly revived, giving credence to the idea that the mammoth could be successfully revived.

Overview

In theory, preserved genetic material found in remains of woolly mammoths could be used to recreate living mammoths, due to advances in molecular biology techniques and the cloning of mammals, begun with Dolly the Sheep in 1996.[1][2][3] Cloning of mammals has improved in the last two decades. To date, no viable mammoth tissue or its intact genome has been found to attempt cloning.

According to Beth Shapiro, a scientist who has taken a central role in the sequencing of the mammoth genome, states in her 2015 book How to Clone a Mammoth: The Science of De-Extinction, that a mammoth will never be cloned, at least not one that is pure mammoth. Nevertheless, the book concludes that we are likely, at some point, to see something that resembles a mammoth.[4] Comparative genomics shows that the mammoth genome matches 99% of the elephant genome, so researchers working in the field aim to engineer an elephant with mammoth genes, that code for the external appearance and traits of a mammoth.[5] The outcome would be an elephant-mammoth hybrid with no more than 1% mammoth genes.[5] Separate projects are working on gradually adding mammoth genes to elephant cells in vitro.[1][2][6]

Colossal Biosciences, founded in 2021, is one biotechnology company that has publicly stated that its project is to genetically resurrect the woolly mammoth, combining its genes with Asian elephant DNA. It has publicly stated that it intends to complete the project by 2027.[7][8]

Cloning

Cloning involves removal of the DNA-containing nucleus of the egg cell of a female elephant, and replacement with a nucleus from woolly mammoth tissue, a process called somatic cell nuclear transfer. For example, Akira Iritani, at the Kyoto University in Japan, reportedly planned to do this.[9] The cell would then be stimulated into dividing, and implanted in a female elephant. The resulting calf would have the genes of the woolly mammoth. However, nobody as of date has found a viable mammoth cell to begin the cloning process, and most scientists doubt that any living cell could have survived freezing in the tundra of the Arctic.[10][11][12][13] Because of their conditions of preservation, the DNA of frozen mammoths has deteriorated significantly over the millennia.[14][15]

Artificial insemination

A second method involves artificially inseminating an elephant egg cell with sperm cells from a frozen woolly mammoth carcass. The resulting offspring would be an elephant–mammoth hybrid, and the process would have to be repeated, so more hybrids could be used in breeding. After several generations of cross-breeding these hybrids, an almost pure woolly mammoth would be produced. Whether the hybrid embryo would be carried through the two-year gestation is unknown; in one case, an Asian elephant and an African elephant produced a live calf named Motty, but it died of defects at less than two weeks old.[16] There is also another fact to consider, that sperm cells of modern mammals are viable for 15 years at most after deep-freezing. This makes this method unfeasible.[15]

Model depicting the calf "Dima", Stuttgart

Gene editing

In April 2015, Swedish scientists published the complete genome (nuclear DNA sequence) of the woolly mammoth.[1][17] Several projects are working on gradually replacing the genes in elephant cells with mammoth genes.[1][2] One such project is that of Harvard University geneticist George M. Church, who is funded by the Long Now Foundation,[1][2] is attempting to create a mammoth–elephant hybrid using DNA from frozen mammoth carcasses. According to the researchers, a mammoth cannot be recreated, but they will try to eventually grow a hybrid elephant with some woolly mammoth traits in an "artificial womb".[18][19] In 2017, George Church said "Actually it would be more like an elephant with a number of mammoth traits. We're not there yet, but it could happen in a couple of years." The creature, sometimes referred as a "mammophant", would be partly elephant, but with features such as small ears, subcutaneous fat, long shaggy hair and cold-adapted blood.[20] The Harvard University team is attempting to study the animals' characteristics in vitro by replacing or editing some specific mammoth genes into Asian elephant skin cells called fibroblasts that have the potential to become embryonic stem cells.[21] By March 2015 and using the new CRISPR DNA editing technique, Church's team had some woolly mammoth genes edited into the genome of an Asian elephant; focusing on cold-resistance initially,[10] the target genes are for the external ear size, subcutaneous fat, hemoglobin, and hair attributes.[21][22] By February 2017, Church's team had made 45 substitutions to the elephant genome.[18] So far his work focuses solely on single cells. In 2021, Church received $15 million in funding and spun off a new company called Colossal.[23]

The Mammoth Genome Project at Pennsylvania State University is also researching the modification of African elephant DNA to create a mammoth–elephant hybrid.[24] If a viable hybrid embryo is obtained by gene editing procedures, implanting it into a female Asian elephant housed in a zoo may be possible,[1] but with the current knowledge and technology, whether the hybrid embryo would be carried through the two-year gestation is unknown.[25][26]

Ethics

Modern elephants are highly gregarious, as shown by these Sri Lankan elephants

If any method is ever successful, a suggestion has been made to introduce the hybrids to a wildlife reserve in Siberia called the Pleistocene Park,[27] but some biologists question the ethics of such recreation attempts.[28] In addition to the technical problems, not much habitat is left that would be suitable for mammoth–elephant hybrids. Because both species are [were] social and gregarious, creating a few specimens would not be ideal. The time and resources required would be enormous, and the scientific benefits would be unclear, suggesting these resources should instead be used to preserve extant elephant species which are endangered.[15][29] The ethics of using elephants as surrogate mothers in hybridisation attempts has also been questioned, as most embryos would not survive, and knowing the exact needs of a hybrid mammoth–elephant calf would be impossible.[12]

Woolly mammoths and sustainability

Researchers from the company Colossal confirmed that their primary goal when trying to revive the woolly mammoth is to better the environment and climate change itself.[30]

See also

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Mammoth genome sequence completed. BBC News, 23 April 2015.
  2. ↑ 2.0 2.1 2.2 2.3 The Long Now Foundation - Revive and Restore.
  3. ↑ Timmons, Jeanne (January 7, 2013). "Could Ancient Giants Be Cloned? Is It Possible, And Is It Wise?". Valley News. http://www.vnews.com/home/3694233-95/cloning-mammoth-mammoths-extinct. 
  4. ↑ De-extinction and Conservation. Gregory E. Kaebnick, and Bruce Jennings. The Hastings Center Report. 26 July 2017
  5. ↑ 5.0 5.1 The Mammoth Genome Project. Pennsylvania State University. Accessed: October 2018.
  6. ↑ "Mammoth: Back from the Dead" . National Geographic Channel.
  7. ↑ Zimmer, Carl (2021-09-13). "A New Company With a Wild Mission: Bring Back the Woolly Mammoth" (in en-US). https://www.nytimes.com/2021/09/13/science/colossal-woolly-mammoth-DNA.html. 
  8. ↑ Michael Greshko (September 13, 2021). "Mammoth-elephant hybrids could be created within the decade. Should they be?". National Geographic. https://www.nationalgeographic.com/science/article/mammoth-elephant-hybrids-could-be-created-within-the-decade-should-they-be. 
  9. ↑ Lendon, Brad (January 17, 2011). "Scientists trying to clone, resurrect extinct mammoth". CNN News. http://news.blogs.cnn.com/2011/01/17/scientists-trying-to-clone-resurrect-extinct-mammoth/comment-page-25/. 
  10. ↑ 10.0 10.1 Landers, Jackson (February 9, 2015). "Can scientists bring mammoths back to life by cloning?". The Washington Post. https://www.washingtonpost.com/national/health-science/can-scientists-bring-mammoths-back-to-life-by-cloning/2015/02/06/2a825c8c-80ae-11e4-81fd-8c4814dfa9d7_story.html. 
  11. ↑ Zimmer, Carl (April 2013). "Bringing Them Back to Life". National Geographic. http://www.nationalgeographic.com/magazine/2013/04/species-revival-bringing-back-extinct-animals/. 
  12. ↑ 12.0 12.1 Loi, Pasqualino; Saragusty, Joseph; Ptak, Grazyna (2014), Holt, William V.; Brown, Janine L.; Comizzoli, Pierre, eds., "Cloning the Mammoth: A Complicated Task or Just a Dream?", Reproductive Sciences in Animal Conservation, Advances in Experimental Medicine and Biology (New York, NY: Springer New York) 753: pp. 489–502, doi:10.1007/978-1-4939-0820-2_19, ISBN 978-1-4939-0819-6, PMID 25091921, http://link.springer.com/10.1007/978-1-4939-0820-2_19, retrieved 2022-10-23 
  13. ↑ Shapiro, 2015. p. 11
  14. ↑ Timmons, Jeanne (January 7, 2013). "Could Ancient Giants Be Cloned? Is It Possible, And Is It Wise?". Valley News. Archived from the original. Error: If you specify |archiveurl=, you must also specify |archivedate=. https://web.archive.org/web/20150330041050/http://www.vnews.com/home/3694233-95/cloning-mammoth-mammoths-extinct. 
  15. ↑ 15.0 15.1 15.2 Lister, 2007. pp. 42–43
  16. ↑ Stone, R. (1999). "Cloning the Woolly Mammoth". Discover Magazine. Archived from the original on 29 July 2014. https://web.archive.org/web/20140729032746/http://discovermagazine.com/1999/apr/cover. 
  17. ↑ Dalén, Love et al. (18 May 2015). "Complete Genomes Reveal Signatures of Demographic and Genetic Declines in the Woolly Mammoth". Current Biology 25 (10): 1395–1400. doi:10.1016/j.cub.2015.04.007. PMID 25913407. 
  18. ↑ 18.0 18.1 Reviving woolly mammoth will take more than 2 years . BBC News. Helen Pilcher, 22 February 2017.
  19. ↑ Kaebnick, Gregory E.; Jennings, Bruce (2017). "De-extinction and Conservation". Hastings Center Report 47: S2–S4. doi:10.1002/hast.744. PMID 28746761. 
  20. ↑ Church, George (February 16, 2017). "Woolly mammoth on verge of resurrection, scientists reveal". The Guardian. http://www.theguardian.com/home/20170216/cloning-mammoth-mammoths-extinct. 
  21. ↑ 21.0 21.1 Webster, Ben (2015-03-23). "Scientist takes mammoth-cloning a step closer". The Sunday Times. http://www.thetimes.co.uk/tto/science/article4390033.ece. 
  22. ↑ Sarah Fecht (24 March 2014), Woolly Mammoth DNA Successfully Spliced Into Elephant Cells, Popular Science, archived from the original on 26 March 2015, https://web.archive.org/web/20150326161051/http://www.popsci.com/woolly-mammoth-dna-brought-life-elephant-cells 
  23. ↑ Herper, Matthew (September 13, 2021). "Return of the mammoth? George Church-backed company launches with $15 million for elephant-sized quest". https://www.statnews.com/2021/09/13/woolly-mammoths-george-church-colossal-launches/. 
  24. ↑ Scientists Hoped to Have Cloned a Living Woolly Mammoth by Now — Why Haven't We? . Sveta McShane. 17 August 2016. Singularity Hub.
  25. ↑ The Plan to Turn Elephants Into Woolly Mammoths Is Already Underway . 21 May 2014.
  26. ↑ "Scientists Sequence Woolly-Mammoth Genome". Pennsylvania State University. Archived from the original on 2008-12-02. https://web.archive.org/web/20081202005200/http://mammoth.psu.edu/press_release.html. Retrieved 6 November 2014. 
  27. ↑ Zimov, S. A. (2005). "Essays on Science and Society: Pleistocene Park: Return of the Mammoth's Ecosystem". Science 308 (5723): 796–798. doi:10.1126/science.1113442. PMID 15879196. http://doc.rero.ch/record/14978/files/PAL_E2127.pdf. 
  28. ↑ An Analysis of Potential Ethical Justifications for Mammoth De-extinction And a Call for Empirical Research. Yasha Rohwer and Emma Marris. Ethics, Policy & Environment; Volume 21, 2018; Issue 1; pages: 127-142.
  29. ↑ Griffin, A. (2015-03-23). "Woolly mammoth could be revived after scientists paste DNA into elephant's genetic code". Archived from the original on 25 September 2015. https://web.archive.org/web/20150925044229/http://www.independent.co.uk/life-style/gadgets-and-tech/news/woolly-mammoth-could-be-revived-after-scientists-paste-dna-into-elephants-genetic-code-10127125.html. 
  30. ↑ Star, The San Juan Daily (November 1, 2021). "A new company with a wild mission: Bring back the woolly mammoth". https://www.sanjuandailystar.com/post/a-new-company-with-a-wild-mission-bring-back-the-woolly-mammoth. 



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