Biology:INCENP
From HandWiki
Short description: Protein-coding gene in the species Homo sapiens
 Generic protein structure example |
| Chromosome passenger complex (CPC) protein INCENP N terminal | |||||||||
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| Identifiers | |||||||||
| Symbol | INCENP_N | ||||||||
| Pfam | PF12178 | ||||||||
| InterPro | IPR022006 | ||||||||
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| Inner centromere protein, ARK binding region | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | INCENP_ARK-bind | ||||||||
| Pfam | PF03941 | ||||||||
| InterPro | IPR005635 | ||||||||
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Inner centromere protein is a protein that in humans is encoded by the INCENP gene.[1][2][3] It is a regulatory protein in the chromosome passenger complex (CPC). It is involved in regulation of the catalytic proteins Aurora B and Aurora C.[4][5][6] It acts in association with two other proteins - Survivin and Borealin. These proteins form a tight three-helical bundle. The N-terminal domain of INCENP is the domain involved in formation of this three-helical bundle while its C-terminal domain is responsible for the interaction with Aurora B.[7][6]
In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins.[8] The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD.
The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle.[9] These include CENPE; MCAK; KID; cytoplasmic dynein (e.g., DYNC1H1); CliPs (e.g. CLIP1); and CENPF/mitosin (CENPF). The inner centromere proteins (INCENPs),[1] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis.[3][10]
Interactions
INCENP has been shown to interact with H2AFZ,[11] Survivin[12] and CDCA8.[13] The ARK binding region has been found to be necessary and sufficient for binding to aurora-related kinase. This interaction has been implicated in the coordination of chromosome segregation with cell division in yeast.[14]
References
- ↑ 1.0 1.1 "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". J Cell Sci 98 (4): 443–61. Sep 1991. doi:10.1242/jcs.98.4.443. PMID 1860899.
- ↑ "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma 110 (2): 65–74. Jul 2001. doi:10.1007/s004120100130. PMID 11453556.
- ↑ 3.0 3.1 "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3619.
- ↑ Sasai, Kaori; Katayama, Hiroshi; Hawke, David H; Sen, Subrata (2016-01-01). "Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex". PLOS ONE 11 (6): e0157305. doi:10.1371/journal.pone.0157305. ISSN 1932-6203. PMID 27332895. Bibcode: 2016PLoSO..1157305S.
- ↑ Li, Xiangyu; Sakashita, Gyosuke; Matsuzaki, Hideki; Sugimoto, Kenji; Kimura, Keiji; Hanaoka, Fumio; Taniguchi, Hisaaki; Furukawa, Koichi et al. (2004-11-01). "Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C". The Journal of Biological Chemistry 279 (45): 47201–47211. doi:10.1074/jbc.m403029200. ISSN 1083-351X. PMID 15316025.
- ↑ 6.0 6.1 Honda, Reiko; Körner, Roman; Nigg, Erich A (2003-08-01). "Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis". Molecular Biology of the Cell 14 (8): 3325–3341. doi:10.1091/mbc.e02-11-0769. ISSN 1939-4586. PMID 12925766.
- ↑ Jeyaprakash, A. Arockia; Klein, Ulf R.; Lindner, Doris; Ebert, Judith; Nigg, Erich A.; Conti, Elena (2007-10-19). "Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together" (in en). Cell 131 (2): 271–285. doi:10.1016/j.cell.2007.07.045. ISSN 0092-8674. PMID 17956729.
- ↑ Choo, K. H. Andy (1997). The centromere. Oxford [Oxfordshire]: Oxford University Press. ISBN 978-0-19-857780-5. https://archive.org/details/centromere0000choo.
- ↑ "Role of nonhistone proteins in the chromosomal events of mitosis". FASEB J. 8 (12): 947–56. September 1994. doi:10.1096/fasebj.8.12.8088460. PMID 8088460.
- ↑ "Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice". Hum. Mol. Genet. 8 (7): 1145–55. July 1999. doi:10.1093/hmg/8.7.1145. PMID 10369859.
- ↑ "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development". EMBO J. 22 (7): 1599–607. April 2003. doi:10.1093/emboj/cdg160. PMID 12660166.
- ↑ "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis". Curr. Biol. 11 (11): 886–90. June 2001. doi:10.1016/S0960-9822(01)00238-X. PMID 11516652.
- ↑ "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle". J. Cell Biol. 166 (2): 179–91. July 2004. doi:10.1083/jcb.200404001. PMID 15249581.
- ↑ "The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis". Mol. Biol. Cell 13 (4): 1132–43. April 2002. doi:10.1091/mbc.01-07-0330. PMID 11950927.
Further reading
- "INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1.". J. Cell Biol. 143 (7): 1763–74. 1999. doi:10.1083/jcb.143.7.1763. PMID 9864353.
- "Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling cytokinesis.". Chromosoma 107 (6–7): 461–70. 1999. doi:10.1007/s004120050330. PMID 9914378.
- "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags.". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3491–6. 2000. doi:10.1073/pnas.97.7.3491. PMID 10737800. Bibcode: 2000PNAS...97.3491D.
- "INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow.". Exp. Cell Res. 262 (2): 122–7. 2001. doi:10.1006/excr.2000.5088. PMID 11139336.
- "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis.". Curr. Biol. 11 (11): 886–90. 2001. doi:10.1016/S0960-9822(01)00238-X. PMID 11516652.
- "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. 2003. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M.
- "Dynamic relocalization of the chromosomal passenger complex proteins inner centromere protein (INCENP) and aurora-B kinase during male mouse meiosis.". J. Cell Sci. 116 (Pt 6): 961–74. 2003. doi:10.1242/jcs.00330. PMID 12584241.
- "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development.". EMBO J. 22 (7): 1599–607. 2003. doi:10.1093/emboj/cdg160. PMID 12660166.
- "Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis.". Mol. Biol. Cell 14 (8): 3325–41. 2004. doi:10.1091/mbc.E02-11-0769. PMID 12925766.
- "Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo.". J. Biol. Chem. 279 (7): 5655–60. 2004. doi:10.1074/jbc.M311299200. PMID 14610074.
- "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. 2004. doi:10.1038/ng1285. PMID 14702039.
- "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle.". J. Cell Biol. 166 (2): 179–91. 2004. doi:10.1083/jcb.200404001. PMID 15249581.
- "Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C.". J. Biol. Chem. 279 (45): 47201–11. 2004. doi:10.1074/jbc.M403029200. PMID 15316025.
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. 2004. doi:10.1101/gr.2596504. PMID 15489334.
- "Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells.". Biochem. J. 389 (Pt 2): 373–81. 2005. doi:10.1042/BJ20050097. PMID 15796717.
- "Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy.". J. Biomed. Sci. 12 (2): 297–310. 2005. doi:10.1007/s11373-005-0980-0. PMID 15917996.
- "Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody.". EMBO Rep. 7 (1): 85–92. 2006. doi:10.1038/sj.embor.7400562. PMID 16239925.
- "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition.". Nat. Cell Biol. 8 (2): 180–7. 2006. doi:10.1038/ncb1350. PMID 16378098.
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