Biology:Pseudouridylate synthase
| pseudouridylate synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 4.2.1.70 | ||||||||
| CAS number | 9023-35-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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The enzyme pseudouridylate synthase (EC 4.2.1.70) catalyzes the chemical reaction
- uracil + D-ribose 5-phosphate [math]\displaystyle{ \rightleftharpoons }[/math] pseudouridine 5′-phosphate + H2O
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is uracil hydro-lyase (adding D-ribose 5-phosphate pseudouridine-5′-phosphate-forming). Other names in common use include pseudouridylic acid synthetase, pseudouridine monophosphate synthetase, 5-ribosyluracil 5-phosphate synthetase, pseudouridylate synthetase, upsilonUMP synthetase, and uracil hydro-lyase (adding D-ribose 5-phosphate). This enzyme participates in pyrimidine metabolism.
Structural studies
As of late 2007, 22 structures have been solved for this class of enzymes, with PDB accession codes 1DJ0, 1K8W, 1KSK, 1KSL, 1KSV, 1PRZ, 1QYU, 1R3E, 1R3F, 1SB7, 1SGV, 1SI7, 1SZW, 1V9F, 1V9K, 1VIO, 1XPI, 1Z2Z, 1ZE1, 1ZE2, 1ZL3, and 2I82.
References
- "Studies on the Biosynthesis of 5-Ribosyluracil 5'-Monophosphate in Tetrahymena Pyriformis". J. Biol. Chem. 239: 1177–87. 1964. PMID 14165924.
- "Studies on pseudouridylic acid synthetase from various sources". Biochim. Biophys. Acta 238 (2): 165–73. 1971. doi:10.1016/0005-2787(71)90082-7. PMID 4936431.
- "Enzymatic synthesis of deoxypseudouridylic acid and a study of certain of its properties". Biochim. Biophys. Acta 281 (4): 481–7. 1972. doi:10.1016/0005-2787(72)90148-7. PMID 4569284.
- "On the biosynthesis of pseudouridine and of pseudouridylic acid in Agrobacterium tumefaciens". Can. J. Biochem. 44 (2): 259–72. 1966. doi:10.1139/o66-029. PMID 5942965.
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