Biology:4-Hydroxy-3-methylbut-2-enyl diphosphate reductase
From HandWiki
Short description: InterPro Family
| 4-Hydroxy-3-methylbut-2-enyl diphosphate reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.17.1.2 | ||||||||
| CAS number | 512789-14-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2, isopentenyl-diphosphate:NADP+ oxidoreductase, LytB, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, HMBPP reductase, IspH, LytB/IspH) is an enzyme in the non-mevalonate pathway. It acts upon (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (or "HMB-PP").[1][2][3][4][5][6]
- (1) isopentenyl diphosphate + NAD(P)+ + H2O [math]\displaystyle{ \rightleftharpoons }[/math] (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+
- (2) dimethylallyl diphosphate + NAD(P)+ + H2O [math]\displaystyle{ \rightleftharpoons }[/math] (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+
4-Hydroxy-3-methylbut-2-enyl diphosphate reductase is an iron-sulfur protein that contains either a [3Fe-4S] or a [4Fe-4S] cluster.
References
- ↑ "Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein". Proceedings of the National Academy of Sciences of the United States of America 99 (3): 1158–63. February 2002. doi:10.1073/pnas.032658999. PMID 11818558. Bibcode: 2002PNAS...99.1158R.
- ↑ "Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli". FEBS Letters 509 (2): 317–22. December 2001. doi:10.1016/s0014-5793(01)03191-x. PMID 11741609.
- ↑ Charon, L.; Pale-Grosdemange, C.; Rohmer, M. (1999). "On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis". Tetrahedron Lett. 40 (40): 7231–7234. doi:10.1016/s0040-4039(99)01531-2.
- ↑ "Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum". FEBS Letters 579 (28): 6433–8. November 2005. doi:10.1016/j.febslet.2005.10.037. PMID 16289098.
- ↑ "Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein". FEBS Letters 541 (1–3): 115–20. April 2003. doi:10.1016/s0014-5793(03)00317-x. PMID 12706830.
- ↑ "IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis". Journal of the American Chemical Society 126 (40): 12847–55. October 2004. doi:10.1021/ja0471727. PMID 15469281.
 |  |
