Biology:ATP5PF
| Mitochondrial ATP synthase coupling factor 6 (F6) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 solution structure of subunit f6 from the peripheral stalk region of atp synthase from bovine heart mitochondria | |||||||||
| Identifiers | |||||||||
| Symbol | ATP-synt_F6 | ||||||||
| Pfam | PF05511 | ||||||||
| InterPro | IPR008387 | ||||||||
| |||||||||
ATP synthase-coupling factor 6, mitochondrial is an enzyme subunit that in humans is encoded by the ATP5PF gene.[1][2][3]
Function
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, FO, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The FO seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the FO complex, required for F1 and FO interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.[3]
The F6 subunit is part of the peripheral stalk that links the F1 and FO complexes together, and which acts as a stator to prevent certain subunits from rotating with the central rotary element. The peripheral stalk differs in subunit composition between mitochondrial, chloroplast and bacterial F-ATPases. In mitochondria, the peripheral stalk is composed of one copy each of subunits OSCP (oligomycin sensitivity conferral protein), F6, b and d.[4] There is no homologue of subunit F6 in bacterial or chloroplast F-ATPase, whose peripheral stalks are composed of one copy of the delta subunit (homologous to OSCP), and two copies of subunit b in bacteria, or one copy each of subunits b and b' in chloroplasts and photosynthetic bacteria.
References
- ↑ "Molecular cloning of cDNA for the import precursor of human coupling factor 6 of H(+)-ATP synthase in mitochondria". Biochemical and Biophysical Research Communications 178 (2): 793–9. Jul 1991. doi:10.1016/0006-291X(91)90178-A. PMID 1830479.
- ↑ "Human mitochondrial ATP synthase: cloning cDNA for the nuclear-encoded precursor of coupling factor 6". Gene 97 (2): 307–10. Jan 1991. doi:10.1016/0378-1119(91)90068-M. PMID 1825642.
- ↑ 3.0 3.1 "Entrez Gene: ATP5PF ATP synthase peripheral stalk subunit F6". https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=522.
- ↑ "Structure of the F1-binding domain of the stator of bovine F1Fo-ATPase and how it binds an alpha-subunit". Journal of Molecular Biology 351 (4): 824–38. Aug 2005. doi:10.1016/j.jmb.2005.06.012. PMID 16045926.
External links
- Human ATP5PF genome location and ATP5PF gene details page in the UCSC Genome Browser.
Further reading
- "F1-ATPase: a highly efficient rotary ATP machine". Essays in Biochemistry 35: 3–18. 2003. doi:10.1042/bse0350003. PMID 12471886.
- "Rotary protein motors". Trends in Cell Biology 13 (3): 114–21. Mar 2003. doi:10.1016/S0962-8924(03)00004-7. PMID 12628343.
- "Understanding ATP synthesis: structure and mechanism of the F1-ATPase (Review)". Molecular Membrane Biology 20 (1): 27–33. 2003. doi:10.1080/0968768031000066532. PMID 12745923.
- "Human liver protein map: a reference database established by microsequencing and gel comparison". Electrophoresis 13 (12): 992–1001. Dec 1992. doi:10.1002/elps.11501301201. PMID 1286669.
- "Sequence analysis and mapping of a novel human mitochondrial ATP synthase subunit 9 cDNA (ATP5G3)". Genomics 24 (2): 375–7. Nov 1994. doi:10.1006/geno.1994.1631. PMID 7698763.
- "Energy transduction in ATP synthase". Nature 391 (6666): 510–3. Jan 1998. doi:10.1038/35185. PMID 9461222. Bibcode: 1998Natur.391..510E.
- "Assignment of an oligomycin-resistance locus to human chromosome 10". Somatic Cell Genetics 8 (2): 223–44. Mar 1982. doi:10.1007/BF01538679. PMID 9732751.
- "Energy transduction in the F1 motor of ATP synthase". Nature 396 (6708): 279–82. Nov 1998. doi:10.1038/24409. PMID 9834036. Bibcode: 1998Natur.396..279W.
- "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research 11 (3): 422–35. Mar 2001. doi:10.1101/gr.GR1547R. PMID 11230166.
- "Plasma concentration of coupling factor 6 and cardiovascular events in patients with end-stage renal disease". Kidney International 64 (6): 2291–7. Dec 2003. doi:10.1046/j.1523-1755.2003.00334.x. PMID 14633154.
- "Molecular motors: turning the ATP motor". Nature 427 (6973): 407–8. Jan 2004. doi:10.1038/427407b. PMID 14749816. Bibcode: 2004Natur.427..407C.
- "Transmission and prenatal diagnosis of the T9176C mitochondrial DNA mutation". Molecular Human Reproduction 11 (3): 223–8. Mar 2005. doi:10.1093/molehr/gah152. PMID 15709156.
 |  |
