Biology:Apo-beta-carotenoid-14',13'-dioxygenase

Identifiers
EC number	1.13.11.67
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Apo-beta-carotenoid-14',13'-dioxygenase (EC 1.13.11.67 is an enzyme that catalyzes the chemical reaction

8'-apo-beta-carotenol + O₂ [math]\displaystyle{ \rightleftharpoons }[/math] 14'-apo-beta-carotenal + uncharacterized product

Thus, the two substrates of this enzyme are 8'-apo-beta-carotenol and oxygen, whereas its two products are 14'-apo-beta-carotenal and an uncharacterized product that may be (3E,5E)-7-hydroxy-6-methylhepta-3,5-dien-2-one.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O with incorporation of one atom of oxygen (internal monooxygenases o internal mixed-function oxidases). The systematic name of this enzyme class is 8'-apo-beta-carotenol:O2 oxidoreductase.

References

• Daruwalla, A.; Zhang, J.; Lee, H. J.; Khadka, N.; Farquhar, E. R.; Shi, W.; von Lintig, J.; Kiser, P. D. (2020). "Structural basis for carotenoid cleavage by an archaeal carotenoid dioxygenase". Proceedings of the National Academy of Sciences of the United States of America 117 (33): 19914–19925. doi:10.1073/pnas.2004116117. PMID 32747548. Bibcode: 2020PNAS..11719914D. 
• "Enzymatic oxidation of beta-apo-8'-carotenol to beta-apo-14'-carotenal by an enzyme different from beta-carotene-15,15'-dioxygenase". Biochemistry. Biokhimiia 62 (7): 787–92. July 1997. PMID 9331970.