Biology:Conformational change

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Short description: Change in the shape of a macromolecule, often induced by environmental factors
Conformational changes can elicit the motion of a protein complex. Kinesin walking on a microtubule is a molecular biological machine using protein domain dynamics on nanoscales
Main page: Protein dynamics

In biochemistry, a conformational change is a change in the shape of a macromolecule, often induced by environmental factors.

A macromolecule is usually flexible and dynamic. Its shape can change in response to changes in its environment or other factors; each possible shape is called a conformation, and a transition between them is called a conformational change. Factors that may induce such changes include temperature, pH, voltage, light in chromophores, concentration of ions, phosphorylation, or the binding of a ligand. Transitions between these states occur on a variety of length scales (tenths of Å to nm) and time scales (ns to s), and have been linked to functionally relevant phenomena such as allosteric signaling[1] and enzyme catalysis.[2]

Laboratory analysis

Many biophysical techniques such as crystallography, NMR, electron paramagnetic resonance (EPR) using spin label techniques, circular dichroism (CD), hydrogen exchange, and FRET can be used to study macromolecular conformational change. Dual-polarization interferometry is a benchtop technique capable of providing information about conformational changes in biomolecules.[3]

A specific nonlinear optical technique called second-harmonic generation (SHG) has been recently applied to the study of conformational change in proteins.[4] In this method, a second-harmonic-active probe is placed at a site that undergoes motion in the protein by mutagenesis or non-site-specific attachment, and the protein is adsorbed or specifically immobilized to a surface. A change in protein conformation produces a change in the net orientation of the dye relative to the surface plane and therefore the intensity of the second harmonic beam. In a protein sample with a well-defined orientation, the tilt angle of the probe can be quantitatively determined, in real space and real time. Second-harmonic-active unnatural amino acids can also be used as probes.[citation needed]

Another method applies electro-switchable biosurfaces where proteins are placed on top of short DNA molecules which are then dragged through a buffer solution by application of alternating electrical potentials. By measuring their speed which ultimately depends on their hydrodynamic friction, conformational changes can be visualized.[citation needed]

"Nanoantennas" made out of DNA – a novel type of nano-scale optical antenna – can be attached to proteins and produce a signal via fluorescence for their distinct conformational changes.[5][6]

Computational analysis

X-ray crystallography can provide information about changes in conformation at the atomic level, but the expense and difficulty of such experiments make computational methods an attractive alternative.[7] Normal mode analysis with elastic network models, such as the Gaussian network model, can be used to probe molecular dynamics trajectories as well as known structures.[8][9] ProDy is a popular tool for such analysis.[10]

Examples

Conformational changes are important for:

See also

References

  1. ↑ "Proteins move! Protein dynamics and long-range allostery in cell signaling". Protein Structure and Diseases. 83. 2011. 163–221. doi:10.1016/B978-0-12-381262-9.00005-7. ISBN 9780123812629. 
  2. ↑ "Hidden alternative structures of proline isomerase essential for catalysis". Nature 462 (7273): 669–73. December 2009. doi:10.1038/nature08615. PMID 19956261. Bibcode: 2009Natur.462..669F. 
  3. ↑ "Real time, high resolution studies of protein adsorption and structure at the solid–liquid interface using dual polarization interferometry". Journal of Physics: Condensed Matter 16 (26): S2493–S2496. 2004-06-19. doi:10.1088/0953-8984/16/26/023. ISSN 0953-8984. Bibcode: 2004JPCM...16S2493F. https://iopscience.iop.org/article/10.1088/0953-8984/16/26/023. 
  4. ↑ "A second-harmonic-active unnatural amino acid as a structural probe of biomolecules on surfaces". The Journal of Physical Chemistry B 112 (47): 15103–7. November 2008. doi:10.1021/jp803703m. PMID 18928314. 
  5. ↑ "Chemists use DNA to build the world's tiniest antenna" (in en). University of Montreal. https://phys.org/news/2022-01-chemists-dna-world-tiniest-antenna.html. 
  6. ↑ Harroun, Scott G.; Lauzon, Dominic; Ebert, Maximilian C. C. J. C.; Desrosiers, Arnaud; Wang, Xiaomeng; Vallée-Bélisle, Alexis (January 2022). "Monitoring protein conformational changes using fluorescent nanoantennas" (in en). Nature Methods 19 (1): 71–80. doi:10.1038/s41592-021-01355-5. ISSN 1548-7105. PMID 34969985. 
  7. ↑ "Chapter 3. High-throughput protein purification for x-ray crystallography and NMR". Advances in Protein Chemistry and Structural Biology 75: 85–105. 2008-01-01. doi:10.1016/S0065-3233(07)75003-9. PMID 20731990. 
  8. ↑ "Long-range correlation in protein dynamics: Confirmation by structural data and normal mode analysis". PLOS Computational Biology 16 (2): e1007670. February 2020. doi:10.1371/journal.pcbi.1007670. PMID 32053592. Bibcode: 2020PLSCB..16E7670T. 
  9. ↑ "A comparative study of motor-protein motions by using a simple elastic-network model". Proceedings of the National Academy of Sciences of the United States of America 100 (23): 13253–8. November 2003. doi:10.1073/pnas.2235686100. PMID 14585932. Bibcode: 2003PNAS..10013253Z. 
  10. ↑ "ProDy: protein dynamics inferred from theory and experiments". Bioinformatics 27 (11): 1575–7. June 2011. doi:10.1093/bioinformatics/btr168. PMID 21471012. 
  11. ↑ ABC Transporters in Microorganisms. Caister Academic. 2009. ISBN 978-1-904455-49-3. 
  12. ↑ "At the dawn of the 21st century: Is dynamics the missing link for understanding enzyme catalysis?". Proteins 78 (6): 1339–75. May 2010. doi:10.1002/prot.22654. PMID 20099310. 
  13. ↑ Mechanics of motor proteins and the cytoskeleton (1st ed.). Sunderland,MA: Sinauer Associates. 2001. ISBN 9780878933334. 
  14. ↑ "Controllable Activation of Nanoscale Dynamics in a Disordered Protein Alters Binding Kinetics". Journal of Molecular Biology 429 (7): 987–998. April 2017. doi:10.1016/j.jmb.2017.03.003. PMID 28285124. 
  15. ↑ Ion Channels of Excitable Membranes (3rd ed.). Sunderland, Mass: Sinauer Associates, Inc.. 2001. pp. 5. ISBN 978-0-87893-321-1. 
  16. ↑ "α-Catenin Structure and Nanoscale Dynamics in Solution and in Complex with F-Actin". Biophysical Journal 115 (4): 642–654. August 2018. doi:10.1016/j.bpj.2018.07.005. PMID 30037495. Bibcode: 2018BpJ...115..642N. 
  17. ↑ Biochemistry. Voet, Judith G. (4th ed.). Hoboken, NJ: John Wiley & Sons. 2011. ISBN 9780470570951. OCLC 690489261. 
  18. ↑ Kimball's Biology pages , Cell Membranes
  19. ↑ Bacteria in Biology, Biotechnology and Medicine (5th ed.). New York: Wiley. 1999. ISBN 978-0-471-98880-9. 

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