Biology:HA-tag
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA-tag is derived from the HA-molecule corresponding to amino acids 98-106. HA-tag has been extensively used as a general epitope tag in expression vectors. [1] Many recombinant proteins have been engineered to express the HA-tag, which does not generally appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation and purification of the protein of interest.[2]
The HA-tag is not suitable for detection or purification of proteins from apoptotic cells since it is cleaved by Caspase-3 and / or Caspase-7 after its sequence DVPD, causing it to lose its immunoreactivity.[3] Labeling of endogenous proteins with HA-tag using CRISPR was recently accomplished in-vivo in differentiated neurons.[4]
Sequence
The DNA sequences for the HA-tag include: 5'-TAC-CCA-TAC-GAT-GTT-CCA-GAT-TAC-GCT-3' or 5'-TAT-CCA-TAT-GAT-GTT-CCA-GAT-TAT-GCT-3'. The resulting amino acid sequence is YPYDVPDYA (Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala).[citation needed]
See also
References
- ↑ "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.". Mol Cell Biol 8 (5): 2159–65. 1988. doi:10.1128/mcb.8.5.2159-2165.1988. PMID 2455217.
- ↑ "Anti-HA Tag Antibody Updated 28/10/2021". Merck Millipore. https://www.merckmillipore.com/GB/en/product/Anti-HA-Tag-Antibody,MM_NF-05-904.
- ↑ "The HA tag is cleaved and loses immunoreactivity during apoptosis". Nature Methods 4 (2): 107–8. February 2007. doi:10.1038/nmeth0207-107. PMID 17264856.
- ↑ "High Throughput, High Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing". Cell 165 (7): 1803–1817. 2016. doi:10.1016/j.cell.2016.04.044. PMID 27180908.
Further reading
- "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method". Molecular and Cellular Biology 8 (5): 2159–65. May 1988. doi:10.1128/MCB.8.5.2159. PMID 2455217.
Original source: https://en.wikipedia.org/wiki/HA-tag.
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