Biology:mRNA (guanine-N7-)-methyltransferase
From HandWiki
Short description: Enzyme
| mRNA (guanine-N7-)-methyltransferase | |||||||||
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| Identifiers | |||||||||
| EC number | 2.1.1.56 | ||||||||
| CAS number | 56941-25-4 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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 Generic protein structure example |
In enzymology, a mRNA (guanine-N7-)-methyltransferase also known as mRNA cap guanine-N7 methyltransferase is an enzyme that catalyzes the chemical reaction
- S-adenosyl-L-methionine + G(5')pppR-RNA [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + m7G(5')pppR-RNA (mRNA containing an N7-methylguanine cap)
Thus, the two substrates of this enzyme are S-adenosyl methionine and G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppR-RNA. This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases.
In humans, mRNA cap guanine-N7 methyltransferase is encoded by the RNMT gene.[1][2][3]
Nomenclature
The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (guanine-N7-)-methyltransferase. Other names in common use include:
- messenger ribonucleate guanine 7-methyltransferase,
- guanine-7-methyltransferase,
- messenger RNA guanine 7-methyltransferase, and
- S-adenosyl-L-methionine:mRNA (guanine-7-N-)-methyltransferase.
- cap MTase
See also
References
- ↑ "Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively". Genomics 54 (2): 351–3. Jan 1999. doi:10.1006/geno.1998.5604. PMID 9828141.
- ↑ "Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes". J Biol Chem 273 (34): 21443–6. Sep 1998. doi:10.1074/jbc.273.34.21443. PMID 9705270.
- ↑ "Entrez Gene: RNMT RNA (guanine-7-) methyltransferase". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8731.
Further reading
- "Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus". Proc. Natl. Acad. Sci. U.S.A. 72 (7): 2525–9. 1975. doi:10.1073/pnas.72.7.2525. PMID 1058472. Bibcode: 1975PNAS...72.2525E.
- "Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates". Biochemistry 17 (6): 977–82. 1978. doi:10.1021/bi00599a005. PMID 629955.
- "Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions". J. Biol. Chem. 250 (24): 9330–5. 1975. doi:10.1016/S0021-9258(19)40647-9. PMID 1194287.
- "Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions". J. Biol. Chem. 250 (24): 9322–9. 1975. doi:10.1016/S0021-9258(19)40646-7. PMID 1194286.
- "Post-transcriptional modifications of mRNA. Purification and characterization of cap I and cap II RNA (nucleoside-2'-)-methyltransferases from HeLa cells.". J. Biol. Chem. 256 (19): 10054–60. 1981. doi:10.1016/S0021-9258(19)68740-5. PMID 7275966.
- Ishikawa K; Nagase T; Nakajima D et al. (1998). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 4 (5): 307–13. doi:10.1093/dnares/4.5.307. PMID 9455477.
- "Cloning and characterization of three human cDNAs encoding mRNA (guanine-7-)-methyltransferase, an mRNA cap methylase". Biochem. Biophys. Res. Commun. 251 (1): 27–34. 1998. doi:10.1006/bbrc.1998.9402. PMID 9790902.
- "Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes". J. Biol. Chem. 274 (23): 16553–62. 1999. doi:10.1074/jbc.274.23.16553. PMID 10347220.
- Yamada-Okabe T; Mio T; Kashima Y et al. (2000). "The Candida albicans gene for mRNA 5-cap methyltransferase: identification of additional residues essential for catalysis". Microbiology 145 (11): 3023–33. doi:10.1099/00221287-145-11-3023. PMID 10589710.
- "Cap methyltransferase selective binding and methylation of GpppG-RNA are stimulated by importin-α". Genes Dev. 14 (23): 2944–9. 2001. doi:10.1101/gad.848200. PMID 11114884.
- Strausberg RL; Feingold EA; Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M.
- Gerhard DS; Wagner L; Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
- "Human mRNA Cap Methyltransferase: Alternative Nuclear Localization Signal Motifs Ensure Nuclear Localization Required for Viability". Mol. Cell. Biol. 25 (7): 2644–9. 2005. doi:10.1128/MCB.25.7.2644-2649.2005. PMID 15767670.
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