Biology:Obscurin
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Obscurin is a protein that in humans is encoded by the OBSCN gene.[1][2][3] Obscurin belongs to the family of giant sarcomeric signaling proteins that includes titin and nebulin. Obscurin is expressed in cardiac and skeletal muscle, and plays a role in the organization of myofibrils during sarcomere assembly. A mutation in the OBSCN gene has been associated with hypertrophic cardiomyopathy and altered obscurin protein properties have been associated with other muscle diseases.
Structure
Human obscurin may exist as multiple splice variants of approximately 720 kDa,[4][5][6][7][8] however the full-length nature of only one has been described to date.[9] Obscurin is expressed in cardiac and skeletal muscle. The obscurin gene spans more than 150 kb, contains over 80 exons.[10] The encoded protein contains 68 Ig domains, 2 fibronectin domains, 1 calcium/calmodulin-binding domain, 1 RhoGEF domain with an associated PH domain, and 2 serine-threonine kinase domains.[9] The dominant location of obscurin in mature myofibrils is at the sarcomeric M-band.[9][11] Titin, obscurin, obscurin-like-1 and myomesin form a ternary complex at sarcomeric M-bands that is critical for sarcomere mechanics.[12]
Function
Obscurin belongs to the family of giant sarcomeric signaling proteins that includes titin and nebulin, and may have a role in the organization of myofibrils during assembly and may mediate interactions between the sarcoplasmic reticulum and myofibrils. Obscurin is the major cytoplasmic ligand for small ankyrin 1 (sANK1), a sarcoplasmic reticular protein, and the scaffolding function of obscurin appears to prevent degradation of sANK1.[13] These data indicate that obscurin serves as a signaling link between the sarcomeric and sarcoplasmic reticular domains,[14][15] Obscurin plays a role in the formation of new sarcomeres during myofibril assembly.[16] specifically, at the sarcomeric periphery where sites of initiation and progression of myofibrilogenesis lie.[17][18] Obscurin appears to be necessary for the proper incorporation of myosin filaments into sarcomeres and in the assembly of A-bands.[11][19] Moreover, the kinase domains of obscurin are enzymatically active and appear to be involved in the regulation of cell adhesion.[20]
Clinical significance
Obscurin has been shown to exhibit a disease-related isoform switch in patients with dilated cardiomyopathy.[21] An obscurin mutation Arg4344Gln was identified in patients with hypertrophic cardiomyopathy, which disrupted binding of obscurin to the Z9-Z10 domains of titin.[22] A later study, however, was not able to reproduce this effect.[23] Due to lack of mechanistic evidence and the high prevalence among African Americans, the Arg4344Gln variant is currently not considered to be pathogenic.[24][25] Mutations found the gene encoding titin in patients with limb-girdle muscular dystrophy 2J or Salih myopathy decrease the ability of titin to bind obscurin, suggesting that this may be causative in disease manifestation.[26]
Interactions
Obscurin has been shown to interact with Titin,[1][27] specifically, with the Novex-3 of Titin, a 6.5 kb exon located upstream of the cardiac-specific N2B exon.[28] The C-terminal region of Obscurin interacts with the cytoplasmic domain of small ankyrin 1[29][30] and with the exon 43' region of ankyrin B.[31] The Ig3 of obscurin binds myomesin at the linker between My4 and My5.[26]
References
- ↑ 1.0 1.1 "Obscurin, a giant sarcomeric Rho guanine nucleotide exchange factor protein involved in sarcomere assembly". The Journal of Cell Biology 154 (1): 123–36. Jul 2001. doi:10.1083/jcb.200102110. PMID 11448995.
- ↑ "Identification, tissue expression and chromosomal localization of human Obscurin-MLCK, a member of the titin and Dbl families of myosin light chain kinases". Gene 282 (1–2): 237–46. Jan 2002. doi:10.1016/S0378-1119(01)00795-8. PMID 11814696.
- ↑ "Entrez Gene: OBSCN obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=84033.
- ↑ "Q5VST9". http://www.heartproteome.org/copa/ProteinInfo.aspx?QType=Protein%20ID&QValue=Q5VST9.
- ↑ "Q5VST9-6". http://www.heartproteome.org/copa/ProteinInfo.aspx?QType=Protein%20ID&QValue=Q5VST9-6.
- ↑ "Q5VST9-2". http://www.heartproteome.org/copa/ProteinInfo.aspx?QType=Protein%20ID&QValue=Q5VST9-2.
- ↑ "Q5VST9-3". http://www.heartproteome.org/copa/ProteinInfo.aspx?QType=Protein%20ID&QValue=Q5VST9-3.
- ↑ "Q5VST9-5". http://www.heartproteome.org/copa/ProteinInfo.aspx?QType=Protein%20ID&QValue=Q5VST9-5.
- ↑ 9.0 9.1 9.2 "Obscurin, a giant sarcomeric Rho guanine nucleotide exchange factor protein involved in sarcomere assembly". The Journal of Cell Biology 154 (1): 123–36. Jul 2001. doi:10.1083/jcb.200102110. PMID 11448995.
- ↑ "Complete human gene structure of obscurin: implications for isoform generation by differential splicing". Journal of Muscle Research and Cell Motility 26 (6–8): 427–34. 2005. doi:10.1007/s10974-005-9025-6. PMID 16625316.
- ↑ 11.0 11.1 "New aspects of obscurin in human striated muscles". Histochemistry and Cell Biology 130 (1): 91–103. Jul 2008. doi:10.1007/s00418-008-0413-z. PMID 18350308.
- ↑ "Structural insight into M-band assembly and mechanics from the titin-obscurin-like-1 complex". Proceedings of the National Academy of Sciences of the United States of America 107 (7): 2908–13. Feb 2010. doi:10.1073/pnas.0913736107. PMID 20133654. Bibcode: 2010PNAS..107.2908P.
- ↑ "Obscurin and KCTD6 regulate cullin-dependent small ankyrin-1 (sAnk1.5) protein turnover". Molecular Biology of the Cell 23 (13): 2490–504. Jul 2012. doi:10.1091/mbc.E12-01-0052. PMID 22573887.
- ↑ "Binding of an ankyrin-1 isoform to obscurin suggests a molecular link between the sarcoplasmic reticulum and myofibrils in striated muscles". The Journal of Cell Biology 160 (2): 245–53. Jan 2003. doi:10.1083/jcb.200208109. PMID 12527750.
- ↑ "Obscurin is a ligand for small ankyrin 1 in skeletal muscle". Molecular Biology of the Cell 14 (3): 1138–48. Mar 2003. doi:10.1091/mbc.E02-07-0411. PMID 12631729.
- ↑ "Dynamics of obscurin localization during differentiation and remodeling of cardiac myocytes: obscurin as an integrator of myofibrillar structure". The Journal of Histochemistry and Cytochemistry 52 (9): 1117–27. Sep 2004. doi:10.1369/jhc.3A6183.2004. PMID 15314079.
- ↑ "Rapid response of cardiac obscurin gene cluster to aortic stenosis: differential activation of Rho-GEF and MLCK and involvement in hypertrophic growth". Biochemical and Biophysical Research Communications 310 (3): 910–8. Oct 2003. doi:10.1016/j.bbrc.2003.09.035. PMID 14550291.
- ↑ "Early incorporation of obscurin into nascent sarcomeres: implication for myofibril assembly during cardiac myogenesis". Histochemistry and Cell Biology 129 (4): 463–78. Apr 2008. doi:10.1007/s00418-008-0378-y. PMID 18219491.
- ↑ "Essential role of obscurin in cardiac myofibrillogenesis and hypertrophic response: evidence from small interfering RNA-mediated gene silencing". Histochemistry and Cell Biology 125 (3): 227–38. Mar 2006. doi:10.1007/s00418-005-0069-x. PMID 16205939. https://deepblue.lib.umich.edu/bitstream/2027.42/47398/1/418_2005_Article_69.pdf.
- ↑ "The kinase domains of obscurin interact with intercellular adhesion proteins". FASEB Journal 27 (5): 2001–12. May 2013. doi:10.1096/fj.12-221317. PMID 23392350.
- ↑ "Passive stiffness changes caused by upregulation of compliant titin isoforms in human dilated cardiomyopathy hearts". Circulation Research 95 (7): 708–16. October 2004. doi:10.1161/01.RES.0000143901.37063.2f. PMID 15345656.
- ↑ "Structural analysis of obscurin gene in hypertrophic cardiomyopathy". Biochemical and Biophysical Research Communications 362 (2): 281–7. October 2007. doi:10.1016/j.bbrc.2007.07.183. PMID 17716621.
- ↑ "When is an obscurin variant pathogenic? The impact of Arg4344Gln and Arg4444Trp variants on protein-protein interactions and protein stability". Human Molecular Genetics 30 (12): 1131–1141. June 2021. doi:10.1093/hmg/ddab010. PMID 33438037.
- ↑ "When is an obscurin variant pathogenic? The impact of Arg4344Gln and Arg4444Trp variants on protein-protein interactions and protein stability". Human Molecular Genetics 30 (12): 1131–1141. June 2021. doi:10.1093/hmg/ddab010. PMID 33438037.
- ↑ "Genetic Misdiagnoses and the Potential for Health Disparities". The New England Journal of Medicine 375 (7): 655–65. August 2016. doi:10.1056/NEJMsa1507092. PMID 27532831.
- ↑ 26.0 26.1 "Interactions with titin and myomesin target obscurin and obscurin-like 1 to the M-band: implications for hereditary myopathies". Journal of Cell Science 121 (11): 1841–51. June 2008. doi:10.1242/jcs.028019. PMID 18477606.
- ↑ "The crystal structure of the human titin:obscurin complex reveals a conserved yet specific muscle M-band zipper module". Journal of Molecular Biology 427 (4): 718–36. Feb 2015. doi:10.1016/j.jmb.2014.11.019. PMID 25490259.
- ↑ "The complete gene sequence of titin, expression of an unusual approximately 700-kDa titin isoform, and its interaction with obscurin identify a novel Z-line to I-band linking system". Circulation Research 89 (11): 1065–72. Nov 2001. doi:10.1161/hh2301.100981. PMID 11717165.
- ↑ "Obscurin is a ligand for small ankyrin 1 in skeletal muscle". Molecular Biology of the Cell 14 (3): 1138–48. Mar 2003. doi:10.1091/mbc.E02-07-0411. PMID 12631729.
- ↑ "Electrostatic interactions mediate binding of obscurin to small ankyrin 1: biochemical and molecular modeling studies". Journal of Molecular Biology 408 (2): 321–34. Apr 2011. doi:10.1016/j.jmb.2011.01.053. PMID 21333652.
- ↑ "Obscurin targets ankyrin-B and protein phosphatase 2A to the cardiac M-line". The Journal of Biological Chemistry 283 (46): 31968–80. Nov 2008. doi:10.1074/jbc.M806050200. PMID 18782775.
Further reading
- "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research 7 (4): 273–81. Aug 2000. doi:10.1093/dnares/7.4.271. PMID 10997877.
- "The complete gene sequence of titin, expression of an unusual approximately 700-kDa titin isoform, and its interaction with obscurin identify a novel Z-line to I-band linking system". Circulation Research 89 (11): 1065–72. Nov 2001. doi:10.1161/hh2301.100981. PMID 11717165.
- "Binding of an ankyrin-1 isoform to obscurin suggests a molecular link between the sarcoplasmic reticulum and myofibrils in striated muscles". The Journal of Cell Biology 160 (2): 245–53. Jan 2003. doi:10.1083/jcb.200208109. PMID 12527750.
- "Obscurin is a ligand for small ankyrin 1 in skeletal muscle". Molecular Biology of the Cell 14 (3): 1138–48. Mar 2003. doi:10.1091/mbc.E02-07-0411. PMID 12631729.
- "Obscurin regulates the organization of myosin into A bands". American Journal of Physiology. Cell Physiology 287 (1): C209-17. Jul 2004. doi:10.1152/ajpcell.00497.2003. PMID 15013951.
- "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nature Biotechnology 22 (6): 707–16. Jun 2004. doi:10.1038/nbt971. PMID 15146197.
- "Orthologous relationship of obscurin and Unc-89: phylogeny of a novel family of tandem myosin light chain kinases". Development Genes and Evolution 214 (7): 352–9. Jul 2004. doi:10.1007/s00427-004-0413-5. PMID 15185077. https://deepblue.lib.umich.edu/bitstream/2027.42/47514/1/427_2004_Article_413.pdf.
- "Essential role of obscurin in cardiac myofibrillogenesis and hypertrophic response: evidence from small interfering RNA-mediated gene silencing". Histochemistry and Cell Biology 125 (3): 227–38. Mar 2006. doi:10.1007/s00418-005-0069-x. PMID 16205939. https://deepblue.lib.umich.edu/bitstream/2027.42/47398/1/418_2005_Article_69.pdf.
- "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research 16 (1): 55–65. Jan 2006. doi:10.1101/gr.4039406. PMID 16344560.
- "Complete human gene structure of obscurin: implications for isoform generation by differential splicing". Journal of Muscle Research and Cell Motility 26 (6–8): 427–34. 2007. doi:10.1007/s10974-005-9025-6. PMID 16625316.
- "Highly accurate two-gene classifier for differentiating gastrointestinal stromal tumors and leiomyosarcomas". Proceedings of the National Academy of Sciences of the United States of America 104 (9): 3414–9. Feb 2007. doi:10.1073/pnas.0611373104. PMID 17360660. Bibcode: 2007PNAS..104.3414P.
- "Different obscurin isoforms localize to distinct sites at sarcomeres". FEBS Letters 581 (8): 1549–54. Apr 2007. doi:10.1016/j.febslet.2007.03.011. PMID 17382936.
- "Structural analysis of obscurin gene in hypertrophic cardiomyopathy". Biochemical and Biophysical Research Communications 362 (2): 281–7. Oct 2007. doi:10.1016/j.bbrc.2007.07.183. PMID 17716621.
- "Mapping the binding site on small ankyrin 1 for obscurin". The Journal of Biological Chemistry 282 (44): 32384–96. Nov 2007. doi:10.1074/jbc.M704089200. PMID 17720975.
![]() | Original source: https://en.wikipedia.org/wiki/Obscurin.
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