Biology:Phosphomethylpyrimidine synthase
From HandWiki
| Phosphomethylpyrimidine synthase | |||||||||
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 Phosphomethylpyrimidine synthase dimer, Arabidopsis thaliana | |||||||||
| Identifiers | |||||||||
| EC number | 4.1.99.17 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Phosphomethylpyrimidine synthase (EC 4.1.99.17, thiC (gene)) is an enzyme with systematic name 5-amino-1-(5-phospho-D-ribosyl)imidazole formate-lyase (decarboxylating, 4-amino-2-methyl-5-phosphomethylpyrimidine-forming).[1][2][3] This enzyme catalyses the following chemical reaction
- 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine [math]\displaystyle{ \rightleftharpoons }[/math] 4-amino-2-methyl-5-phosphomethylpyrimidine + 5′-deoxyadenosine + L-methionine + formate + CO
This enzyme binds a 4Fe-4S cluster.
The starting material is 5-aminoimidazole ribotide, which undergoes a rearrangement reaction via radical intermediates which incorporate the blue, green and red fragments shown into the product.[3][4]
References
- ↑ "Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily". Nature Chemical Biology 4 (12): 758–65. December 2008. doi:10.1038/nchembio.121. PMID 18953358.
- ↑ "Reaction of AdoMet with ThiC generates a backbone free radical". Biochemistry 48 (2): 217–9. January 2009. doi:10.1021/bi802154j. PMID 19113839.
- ↑ 3.0 3.1 "A "radical dance" in thiamin biosynthesis: mechanistic analysis of the bacterial hydroxymethylpyrimidine phosphate synthase". Angewandte Chemie 49 (46): 8653–6. November 2010. doi:10.1002/anie.201003419. PMID 20886485.
- ↑ Begley, Tadhg P. (2006). "Cofactor biosynthesis: An organic chemist's treasure trove". Natural Product Reports 23 (1): 15–18. doi:10.1039/b207131m. PMID 16453030.
External links
- Phosphomethylpyrimidine+synthase at the US National Library of Medicine Medical Subject Headings (MeSH)
External links
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