Biology:Protein G
| GA-like domain | |||||||||
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| Symbol | ? | ||||||||
| Pfam | PF17573 | ||||||||
| InterPro | IPR035152 | ||||||||
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| Immunoglobulin G-binding protein G | |||||||
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| Identifiers | |||||||
| Organism | |||||||
| Symbol | spg | ||||||
| UniProt | P06654 | ||||||
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Protein G is an immunoglobulin-binding protein expressed in group C and G streptococcal bacteria much like protein A but with differing binding specificities. It is a ~60-kDA (65 kDA for strain G148 and 58 kDa for strain C40)[1] cell surface protein that has found application in purifying antibodies through its binding to the Fab and Fc region. The native molecule also binds albumin, but because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of protein G. This recombinant protein G, either labeled with a fluorophore or a single-stranded DNA strand, was used as a replacement for secondary antibodies in immunofluorescence and super-resolution imaging.[2]
Other antibody binding proteins
In addition to protein G, other immunoglobulin-binding bacterial proteins such as protein A, protein A/G and protein L are all commonly used to purify, immobilize or detect immunoglobulins. Each of these immunoglobulin-binding proteins has a different antibody binding profile in terms of the portion of the antibody that is recognized and the species and type of antibodies it will bind.
Folding of protein G, B1 domain
An ab initio simulation of the protein G B1 domain demonstrates that, as earlier results suggested, this protein initiates folding via a nucleation event in the hydrophobic core residues followed by small adjustments.[3] The folding events are as follows:
- a β-hairpin is formed, stabilized by residues W43, Y45, and F52.
- Residue contacts between residue F30, in an α-helix, and the β-hairpin strengthen.
- Nucleation of the β-sheet starting from residues L5 and F52, occurs.
- The last nucleation residue, Y3, assists in forming the central part of the β-sheet resulting in a globular protein.
The protein G B1 domain is (aka. GB1) often used as part of a fusion protein to keep other domains in solution during experiments in solution (e.g. NMR). Many previously insoluble domains have become soluble with the fusion of the GB1 domain.[4] The domain is 56 residues (approx 8kDa) long. On SDS-PAGE gels the GB1 domain runs at roughly 13.5kDa despite being only 8kDa.[5]
See also
References
- ↑ "Streptococcal protein G. Gene structure and protein binding properties". J Biol Chem 266 (1): 399–405. 1991. doi:10.1016/S0021-9258(18)52448-0. PMID 1985908.
- ↑ Schlichthaerle, Thomas; Ganji, Mahipal; Auer, Alexander; Wade, Orsolya Kimbu; Jungmann, Ralf (2018). "Bacterial-derived antibody binders as small adapters for DNA-PAINT microscopy" (in en). ChemBioChem 20 (8): 1032–1038. doi:10.1002/cbic.201800743. ISSN 1439-7633. PMID 30589198.
- ↑ "Folding pathway of the b1 domain of protein G explored by multiscale modeling". Biophys J 94 (3): 726–36. Feb 2008. doi:10.1529/biophysj.107.116095. PMID 17890394. Bibcode: 2008BpJ....94..726K.
- ↑ Cheng, Yuan; Patel, Dinshaw J. (2004). "An efficient system for small protein expression and refolding". Biochemical and Biophysical Research Communications 317 (2): 401–405. doi:10.1016/j.bbrc.2004.03.068. PMID 15063772.
- ↑ Hartl MJ, Mayr F, Rethwilm A, Wöhrl BM (2010). "Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases.". Retrovirology 7: 5. doi:10.1186/1742-4690-7-5. PMID 20113504.
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