Biology:Pt-barrel
| Orf2-like Prenyltransferase | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
 Crystal structure of a PT-barrel protein. | |||||||||||
| Identifiers | |||||||||||
| Symbol | PTase_Orf2 | ||||||||||
| Pfam | PF11468 | ||||||||||
| InterPro | IPR020965 | ||||||||||
| SCOP2 | 1zb6 / SCOPe / SUPFAM | ||||||||||
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The PT-barrel, is a novel protein fold that was discovered in the crystal structure of the prenyltransferase, Orf2 from Streptomyces sp. strain CL190.[1]
Structure
The PT-barrel consists of a closed β-sheet comprising ten anti-parallel β-strands arranged around a central β-barrel core, itself surrounded by a ring of α-helices forming the outer, solvent exposed surface of the barrel.
The secondary connectivity nearly conforms to an (ααββ)5 classification, but is more specifically described using the (ααββ)4-(αββ)−α nomenclature, where helices 6 and 8, both involved in inter-protein contacts in the crystal lattice, display a helical “kink”. The most hydrophobic section of the PT-barrel is the region residing between the outer surface of the cylindrical β-barrel and the belt of surrounding α-helices. Additionally, a number of hydrophobic residues located inside the barrel accommodate the prenyl tail of the Geranyl-diPhosphate (GPP) and GSPP molecules, while the diphosphate or the thio-diphosphate head groups of substrate and substrate analogs, respectively, point toward the “upper”, more polar end of the barrel where a Mg2+ ion is coordinated. Finally, the bottom of the barrel is capped by a short C-terminal helix (α11).
References
- ↑ "Structural basis for the promiscuous biosynthetic prenylation of aromatic natural products". Nature 435 (7044): 983–7. June 2005. doi:10.1038/nature03668. PMID 15959519. Bibcode: 2005Natur.435..983K.
- Koehl P (July 2005). "Relaxed specificity in aromatic prenyltransferases". Nat. Chem. Biol. 1 (2): 71–2. doi:10.1038/nchembio0705-71. PMID 16408001.
- "Novel prenyltransferase enzymes as a tool for flavonoid prenylation". Trends Pharmacol. Sci. 26 (12): 606–8. December 2005. doi:10.1016/j.tips.2005.09.012. PMID 16229901.
Press Releases
Promiscuous Catalytic Activity Possessed by Novel Enzyme Structure, June 15, 2005
SSRL Science Highlight July 2005
LightSource.org Press Release Number: PR-SSRL-05-3
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