Biology:Sterol 24-C-methyltransferase
| Sterol 24-C-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.1.1.41 | ||||||||
| CAS number | 37257-07-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Sterol 24-C-methyltransferase (EC 2.1.1.41) is an enzyme that catalyzes the chemical reaction
- REDIRECT Template:Chemical reaction
This is a methylation reaction in which zymosterol is converted to fecosterol. The methyl group comes from the cofactor, S-adenosyl methionine (SAM), which loses its methyl group and becomes S-adenosyl-L-homocysteine (SAH).[1][2]
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:zymosterol 24-C-methyltransferase. Other names in common use include Delta24-methyltransferase, Delta24-sterol methyltransferase, zymosterol-24-methyltransferase, S-adenosyl-4-methionine:sterol Delta24-methyltransferase, SMT1, 24-sterol C-methyltransferase, S-adenosyl-L-methionine:Delta24(23)-sterol methyltransferase, and phytosterol methyltransferase.[3] Zymosterol is the preferred substrate for the enzyme but it accepts other sterols with a double bond in the same position in the sidechain, such as desmosterol.[4] It uses glutathione as a cofactor.[5][6]
References
- ↑ "Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast". J. Biol. Chem. 244 (23): 6334–40. 1969. doi:10.1016/S0021-9258(18)63470-2. PMID 5354959.
- ↑ "Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae". Biochim. Biophys. Acta 1299 (3): 313–24. 1996. doi:10.1016/0005-2760(95)00218-9. PMID 8597586.
- ↑ Enzyme 2.1.1.41 at KEGG Pathway Database.
- ↑ "Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: delta 24(25)- to delta 24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae". Arch. Biochem. Biophys. 353 (2): 297–311. 1998. doi:10.1006/abbi.1998.0665. PMID 9606964.
- ↑ "Stereochemical features of C-methylation on the path to Delta24(28)-methylene and Delta24(28)-ethylidene sterols: studies on the recombinant phytosterol methyl transferase from Arabidopsis thaliana". Tetrahedron Lett. 38 (35): 6115–6118. 1997. doi:10.1016/S0040-4039(97)01386-5.
- ↑ "Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis". Eur. J. Biochem. 256 (1): 88–96. 1998. doi:10.1046/j.1432-1327.1998.2560088.x. PMID 9746350.
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