Biology:TRNAIle-lysidine synthase
From HandWiki
| TRNAIle-lysidine synthase | |||||||||
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| Identifiers | |||||||||
| EC number | 6.3.4.19 | ||||||||
| CAS number | 635304-92-6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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TRNAIle-lysidine synthase (EC 6.3.4.19, TilS, mesJ (gene), yacA (gene), isoleucine-specific transfer ribonucleate lysidine synthetase, tRNAIle-lysidine synthetase) is an enzyme with systematic name L-lysine:(tRNAIle2)-cytidine34 ligase (AMP-forming).[1][2][3][4][5] This enzyme catalyses the following chemical reaction
- [tRNAIle2]-cytidine34 + L-lysine + ATP [math]\displaystyle{ \rightleftharpoons }[/math] [tRNAIle2]-lysidine34 + AMP + diphosphate + H2O
The bacterial enzyme modifies the wobble base of the CAU anticodon of tRNAIle at the oxo group in position 2 of cytidine34.
References
- ↑ "molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition". Molecular Cell 19 (2): 235–46. July 2005. doi:10.1016/j.molcel.2005.06.007. PMID 16039592.
- ↑ "The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase". The Journal of Biological Chemistry 284 (15): 9656–62. April 2009. doi:10.1074/jbc.M809013200. PMID 19233850.
- ↑ "Structural basis for lysidine formation by ATP pyrophosphatase accompanied by a lysine-specific loop and a tRNA-recognition domain". Proceedings of the National Academy of Sciences of the United States of America 102 (21): 7487–92. May 2005. doi:10.1073/pnas.0501003102. PMID 15894617.
- ↑ "An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA". Molecular Cell 12 (3): 689–98. September 2003. doi:10.1016/s1097-2765(03)00346-0. PMID 14527414.
- ↑ "Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase". Nature 461 (7267): 1144–8. October 2009. doi:10.1038/nature08474. PMID 19847269.
External links
- TRNAIle-lysidine+synthase at the US National Library of Medicine Medical Subject Headings (MeSH)
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