Biology:Tetrachloroethene reductive dehalogenase
| tetrachloroethene reductive dehalogenase | |||||||||
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| Identifiers | |||||||||
| EC number | 1.97.1.8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a tetrachloroethene reductive dehalogenase (EC 1.97.1.8) is an enzyme that catalyzes the chemical reaction.[1][2] This is a member of reductive dehalogenase enzyme family.
- trichloroethene + chloride + acceptor [math]\displaystyle{ \rightleftharpoons }[/math] tetrachloroethene + reduced acceptor
The 3 substrates of this enzyme are trichloroethene, chloride, and acceptor, whereas its two products are tetrachloroethene and reduced acceptor.
This enzyme belongs to the family of oxidoreductases. The systematic name of this enzyme class is acceptor:trichloroethene oxidoreductase (chlorinating). This enzyme is also called tetrachloroethene reductase. This enzyme participates in tetrachloroethene degradation.
Note that the physiologically relevant reaction actually occurs in the reverse direction from that shown above. In other words, in the bacterial species where this enzyme is found, tetrachloroethene is reductively dechlorinated to trichloroethene and chloride.
This enzyme is one member of a family of enzymes including trichloroethene dehalogenase and vinyl chloride dehalogenase. The other members of this family do not have their own EC numbers at present.
Reductive dehalogenases are key enzymes for anaerobic respiratory process, termed organohalide respiration.
References
- ↑ "Reductive Dehalogenases Come of Age in Biological Destruction of Organohalides". Trends in Biotechnology 33 (10): 595–610. October 2015. doi:10.1016/j.tibtech.2015.07.004. PMID 26409778.
- ↑ "Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation". Frontiers in Microbiology 7: 249. 2016. doi:10.3389/fmicb.2016.00249. PMID 26973626.
Further reading
- "Reductive dechlorination in the energy metabolism of anaerobic bacteria". FEMS Microbiol. Rev. 22 (5): 383–398. 1998. doi:10.1111/j.1574-6976.1998.tb00377.x. http://doc.rero.ch/record/295119/files/22-5-383.pdf.
- "Corrinoid-mediated reduction of tetrachloroethene, trichloroethene, and trichlorofluoroethene in homogeneous aqueous solution: Reaction kinetics and reaction mechanisms". Environ. Sci. Technol. 31 (1): 253–260. 1997. doi:10.1021/es9603867. Bibcode: 1997EnST...31..253G.
- "Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans". The Journal of Biological Chemistry 271 (28): 16515–9. July 1996. doi:10.1074/jbc.271.28.16515. PMID 8663199.
- "Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus". FEBS Letters 409 (3): 421–5. June 1997. doi:10.1016/S0014-5793(97)00520-6. PMID 9224702.
- "The proton/electron ration of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus"". Journal of Bacteriology 178 (8): 2328–33. April 1996. doi:10.1128/jb.178.8.2328-2333.1996. PMID 8636034.
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