Biology:Thiazole synthase

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Thiazole synthase
4y4l.jpg
Thiazole synthase oktamer, Saccharomyces cerevisiae
Identifiers
EC number2.8.1.10
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Thiazole synthase (EC 2.8.1.10, thiG (gene)) is an enzyme with systematic name 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] [math]\displaystyle{ \rightleftharpoons }[/math] 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O

H2S can provide the sulfur in vitro.

References

  1. "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)". Biochemistry 42 (42): 12430–8. October 2003. doi:10.1021/bi034902z. PMID 14567704. 
  2. "The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS". Chemistry & Biology 11 (10): 1373–81. October 2004. doi:10.1016/j.chembiol.2004.08.009. PMID 15489164. 
  3. "The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase". Journal of the American Chemical Society 126 (10): 3091–6. March 2004. doi:10.1021/ja039616p. PMID 15012138. 
  4. "Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex". Biochemistry 43 (37): 11647–57. September 2004. doi:10.1021/bi0488911. PMID 15362849. 
  5. "Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction". Journal of the American Chemical Society 131 (9): 3225–9. March 2009. doi:10.1021/ja806752h. PMID 19216519. 
  6. "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase". Journal of the American Chemical Society 133 (24): 9311–9. June 2011. doi:10.1021/ja1110514. PMID 21534620. 

External links