Physics:DU spectrophotometer

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Short description: First commercially viable UV-visible light absorbance instrument
DU Spectrophotometer, National Technical Laboratories, 1947

The DU spectrophotometer or Beckman DU, introduced in 1941, was the first commercially viable scientific instrument for measuring the amount of ultraviolet light absorbed by a substance. This model of spectrophotometer enabled scientists to easily examine and identify a given substance based on its absorption spectrum, the pattern of light absorbed at different wavelengths. Arnold O. Beckman's National Technical Laboratories (later Beckman Instruments) developed three in-house prototype models (A, B, C) and one limited distribution model (D) before moving to full commercial production with the DU. Approximately 30,000 DU spectrophotometers were manufactured and sold between 1941 and 1976.

Sometimes referred to as a UV–Vis spectrophotometer because it measured both the ultraviolet (UV) and visible spectra, the DU spectrophotometer is credited as being a truly revolutionary technology. It yielded more accurate results than previous methods for determining the chemical composition of a complex substance, and substantially reduced the time needed for an accurate analysis from weeks or hours to minutes. The Beckman DU was essential to several critical secret research projects during World War II, including the development of penicillin and synthetic rubber.

Background

Before the development of the DU spectrophotometer, analysis of a test sample to determine its components was a long, costly, and often inaccurate process. A classical wet laboratory contained a wide variety of complicated apparatus.[1] Test samples were run through a series of awkward and time-consuming qualitative processes to separate out and identify their components. Determining quantitative concentrations of those components in the sample involved further steps. Processes could involve techniques for chemical reactions, precipitations, filtrations and dissolutions.[2]:150[3] Determination of the concentrations of known impurities in a known inorganic substance such as molten iron could be done in under thirty minutes.[2]:26 The determination of complex organic structures such as chlorophyll using wet and dry methods could take decades.[4] :59–60

Spectroscopic methods for observing the absorption of electromagnetic radiation in the visible spectrum were known as early as the 1860s.[4]:65[5]:5 Scientists had observed that light traveling through a medium would be absorbed at different wavelengths, depending on the matter-composition of the medium involved. A white light source would emit light at multiple wavelengths over a range of frequencies. A prism could be used to separate a light source into specific wavelengths. Shining the light through a sample of a material would cause some wavelengths of light to be absorbed, while others would be unaffected and continue to be transmitted. Wavelengths in the resulting absorption spectrum would differ depending upon the atomic and molecular composition if the material involved.[6][7][8]

Spectroscopic methods were predominantly used by physicists and astrophysicists. Spectroscopic techniques were rarely taught in chemistry classes and were unfamiliar to most practicing chemists. Beginning around 1904, Frank Twyman of the London instrument making firm Adam Hilger, Ltd. tried to develop spectroscopic instruments for chemists, but his customer base was consistently made up of physicists rather than chemists. [9]:113–118 By the 1930s he had developed a niche market in metallurgy, where his instruments were well adapted to the types of problems that chemists were solving.[9]:124

By the 1940s, both academic and industrial chemists were becoming increasingly interested in problems involving the composition and detection of biological molecules. Biological molecules, including proteins and nucleic acids, absorb light energy in both the ultraviolet and visible range.[10] The spectrum of visible light was not broad enough to enable scientists to examine substances such as vitamin A.[11] Accurate characterization of complex samples, particularly of biological materials, would require the accurate reading of absorption frequencies in the ultraviolet and infrared (IR) sections of the spectrum in addition to visible light. Existing instruments such as the Cenco "Spectrophotelometer" and the Coleman Model DM Spectrophotometer could not be effectively used to examine wavelengths in the ultraviolet range.[11][12]

The array of equipment needed to measure light energy reaching beyond the visible spectrum towards the ultraviolet could cost a laboratory as much as $3,000, a huge amount in 1940.[2]:149 Repeated readings of a sample were taken to produce photographic plates showing the absorption spectrum of a material at different wavelengths. An experienced human could compare these to the known images to identify a match. Then information from the plates had to be combined to create a graph showing the spectrum as a whole. Ultimately, the accuracy of such approaches was dependent on accurate, consistent development of the photographic plates, and on human visual acuity and practice in reading the wavelengths.[2]:150–151

Development

The DU was developed at National Technical Laboratories (later Beckman Instruments) under the direction of Arnold Orville Beckman, an American chemist and inventor.[13][14] Beginning in 1940, National Technical Laboratories developed three in-house prototype models (A, B, C) and one limited distribution model (D) before moving to full commercial production with the DU in 1941.[5]:6 Beckman's research team was led by Howard Cary, who went on to co-found Applied Physics Corporation (later Cary Instruments) which became one of Beckman Instruments' strongest competitors.[15] Other scientists included Roland Hawes and Kenyon George.[16]

Coleman Instruments had recently coupled a pH meter with an optical phototube unit to examine the visual spectrum (the Coleman Model DM).[10] Beckman had already developed a successful pH meter for measuring acidity of solutions, his company's breakthrough product. Seeing the potential to build upon their existing expertise, Beckman made it a goal to create an easy-to-use integrated instrument which would both register and report specific wavelengths extending into the ultraviolet range. Rather than depending on development of photographic plates, or a human observer's visual ability to detect wavelengths in the absorption spectrum, phototubes would be used to register and report the specific wavelengths that were detected. This had the potential to increase the instrument's accuracy and reliability as well as its speed and ease of use.[2]:149–151

Model A (prototype)

The first prototype Beckman spectrophotometer, the Model A, was created at National Technologies Laboratories in 1940. It used a tungsten light source with a glass Fery prism as a monochromator.[17]:16[18] Tungsten was used for incandescent light filaments because it was strong, withstood heat, and emitted a steady light.[19] Types of light sources differed in the range of wavelengths of light that they emitted. Tungsten lamps were useful in the visible light range but gave poor coverage in the ultraviolet range. However, they had the advantage of being readily available because they were used as automobile headlamps.[17]:17 An external amplifier from the Beckman pH meter and a vacuum tube photocell were used to detect wavelengths.[17]:16

Model B (prototype)

Optical quality quartz crystals

It was quickly realized that a glass dispersive prism was not suitable for use in the ultraviolet spectrum.[2]:153[17]:16 Glass absorbed electromagnetic radiation below 400 millimicrons rather than dispersing it.[20] In the Model B, a quartz prism was substituted for the earlier glass.[2]:153[17]:16

A tangent bar mechanism was used to adjust the monochromator. The mechanism was highly sensitive and required a skilled operator.[17]:16 Only two Model B prototypes were made. One was sold: in February 1941, to the University of California Chemistry department in Los Angeles .[2]:153

The Model B prototype should be distinguished from a later production model of spectrophotometer that was also referred to as the Model "B". The production Model "B" was introduced in 1949 as a less-expensive, simple-to-use alternative to the Beckman DU.[21] It used a glass Fery prism as a chromator and operated in a narrower range, roughly from 320 millimicrons to 950 millimicrons, and 5 to 20 Å.[22]:183–184[23][24][25]

Model C (prototype)

Three Model C instruments were then built, improving the instrument's wavelength resolution. The Model B's rotary cell compartment was replaced with a linear sample chamber. The tangent bar mechanism was replaced by a scroll drive mechanism,[17]:16 which could be more precisely controlled to reset the quartz prism and select the desired wavelength.[10] With this new mechanism, results could be more easily and reliably obtained, without requiring a highly skilled operator. This set the pattern for all of Beckman's later quartz prism instruments.[17]:16 Although only three Model B prototypes were built, all were sold, one to Caltech and the other two to companies in the food industry.[2]:153

Model D (limited production)

Quartz photoelectric spectrophotometer, Cary & Beckman, 1941[26]:687

The A, B, and C prototype models all coupled an external Beckman pH meter to the optical component to obtain readouts. In developing the Model D, Beckman took the direct-coupled amplifier circuit from the pH meter and combined the optical and electronic components in a single housing, making it more economical.[10]

Moving from a prototype to production of the Model D involved challenges. Beckman originally approached Bausch and Lomb about making quartz prisms for the spectrophotometer. When they turned down the opportunity, National Technical Laboratories designed its own optical system, including both a control mechanism and a quartz prism. Large, high optical quality quartz suitable for creating prisms was difficult to obtain. It came from Brazil, and was in demand for wartime radio oscillators. Beckman had to obtain a wartime priority listing for the spectrophotometer to get access to suitable quartz supplies.[17]:17

Beckman had previously attempted to find a source of reliable hydrogen lamps, seeking better sensitivity to wavelengths in the ultraviolet range than was possible with tungsten. As described in July 1941, the Beckman spectrophotometer could use a "hot cathode hydrogen discharge tube" or a tungsten light source interchangeably.[26]:684–685 However, Beckman was still unsatisfied with the available hydrogen lamps. National Technical Laboratories designed its own hydrogen lamp, an anode enclosed in a thin blown-glass window.[17]:17 By December 1941, the in-house design was being used in production of the Model D.[2]:154–155

The instrument's design also required a more sensitive phototube than was commercially available at that time. Beckman was able to obtain small batches of an experimental phototube from RCA for the first Model D instruments.[17]:17

The Model D spectrophotometer, using the experimental RCA phototube, was shown at MIT's Summer Conference on Spectroscopy in July 1941. The paper that Cary and Beckman presented there was published in the Journal of the Optical Society of America. In it, Cary and Beckman compared designs for a modified self-collimating quartz Fery prism, a mirror-collimated quartz Littrow prism, and various gratings.[26]:683 The Littrow prism was a half-prism, which had a mirrored back face, so that the light went through the front face twice.[18][27]:31–34 Use of a tungsten light source with the quartz Littrow prism as a monochromator was reported to minimize light scattering within the instrument.[26]:686

The Model D was the first model to enter actual production. A small number of Model D instruments were sold, beginning in July 1941, before it was superseded by the DU.[2]:153–155[17]:17–18

Model DU

Disassembled absorption cell and photo-tube compartment, Cary & Beckman, 1941[26]:687

When RCA could not meet Beckman's demand for experimental phototubes, National Technical Laboratories again had to design its own components in-house.[17]:18 They developed a pair of phototubes, sensitive to the red and blue areas of the spectrum, capable of amplifying the signals they received.[28]:230 With the incorporation of Beckman's UV-sensitive phototubes, the Model D became the Model DU UV–Vis spectrophotometer.[17]:18 Its designation as a "UV–Vis" spectrophotometer indicates its ability to measure light in both the visible and ultraviolet spectra.[29]

The DU was the first commercially viable scientific instrument for measuring the amount of ultraviolet light absorbed by a substance.[2]:148 [5]:10 As he had done with the pH meter, Beckman had replaced an array of complicated equipment with a single, easy-to-use instrument. One of the first fully integrated instruments[17]:11 or "black boxes" used in modern chemical laboratories,[30] it sold for $723 in 1941.[12]

It is generally assumed that the "DU" in the name was a combination of "D" for the Model D on which it was based, and "U" for the ultraviolet spectrum. However, it has been suggested that "DU" may also reference Beckman's fraternity at the University of Illinois, Delta Upsilon, whose members were called "DU"s.[31]

A publication in the scholarly literature compared the optical quality of the DU to the Cary 14 Spectrophotometer, another leading UV–Vis spectrophotometer of the time.[32]

Design

Model DU spectrophotometer – exploded view, Beckman manual, 1954


From 1941 until 1976, when it was discontinued, the Model DU spectrophotometer was built upon what was essentially the same design.[12] It was a single beam instrument.[16]:11[33] The DU spectrophotometers used a quartz prism to separate light from a lamp into its absorption spectrum and a phototube to electrically measure the light energy across the spectrum. This allowed the user to plot the light absorption spectrum of a substance to obtain a standardized "fingerprint" characteristic of a compound.[2]:151 [34][35] All modern UV–Vis spectrophotometer are built on the same basic principles as the DU spectrophotometer.[29]


Diagram of Model DU optical system, Beckman manual, 1954
"Light from the tungsten lamp is focused by the condensing mirror and directed in a beam to the diagonal slit entrance mirror. The entrance mirror deflects the light through the entrance slit and into the monochromator to the collimating mirror. Light falling on the collimating mirror is rendered parallel and reflected to the quartz prism where it undergoes refraction. The back surface of the prism is aluminized so that light refracted at the first surface is reflected back through the prism, undergoing further refraction as it emerges from the prism. The desired wavelength of light is selected by rotating the Wavelength Selector which adjusts the position of the prism. The spectrum is directed back to the collimating mirror which centers the chosen wavelength on the exit slit and sample. Light passing through the sample strikes the phototube, causing a current gain. The current gain is amplified and registered on the null meter." Model DU Optical System[36]:3

Although the default light source for the instrument was tungsten, a hydrogen or mercury lamp could be substituted depending on the optimal range of measurement for which the instrument was to be used.[36] :3 The tungsten lamp was suitable for transmittance of wavelengths between 320 and 1000 millimicrons; the hydrogen lamp for 220 to 320 millimicrons, and the mercury lamp for checking the calibration of the spectrophotometer.[36]:6


Minimum spectral band widths, Cary & Beckman, 1941

As advertised in the 1941 News Edition of the American Chemical Society, the Beckman Spectrophotometer used an autocollimating quartz crystal prism for a monochromator, capable of covering a range from the ultraviolet (200 millimicrons) to the infrared (2000 millimicrons), with a nominal bandwidth of 2 millimicrons or less for most of its spectral range. The slit mechanism was continuously adjustable from .01 to 2.0 mm and claimed to have less than 1/10% of stray light over most of the spectral range. It featured an easy-to-read wavelength scale, simultaneously reporting % Transmission and Density information.[37]

The sample holder held up to 4 cells.[36]:3[37] Cells could be moved into the light path via an external control, allowing the user to take multiple readings without opening the cell compartment.[36]:3 As described in the DU's manual, absorbance measurements of a sample were made in comparison to a blank, or standard, "a solution identical in composition with the sample except that the absorbing material being measured is absent."[36]:24 The standard could be a cell filled with a solvent such as distilled water[36]:24 or a prepared solvent of a known concentration.[27]:30–31 At each wavelength two measurements are made: with the sample and with the standard in the light beam. This enables the ratio, transmittance, to be obtained. For quantitative measurements transmittance is converted to absorbance which is proportional to the solute concentration according to Beer's law. This makes possible the quantitative determination of the amount of a substance in solution.[38]

The user could also switch between phototubes without removing the sample holder. A 1941 advertisement indicates that three types of phototubes were available, with maximum sensitivity to red, blue and ultraviolet light ranges.[37]

The 1954 DU spectrophotometer differs in that it claims to be useful from 200 to 1000 millimicrons,[36]:2 and does not mention the ultraviolet phototube.[36]:3 The wavelength selector, however, still ranged from 200 to 2000 millimicrons.[36]:4 and an "Ultraviolet accessory set" was available.[36]:25 This shift away from using the DU for infrared measurement is understandable, since by 1954 Beckman Instruments was marketing a separate infrared spectrophotometer. Beckman developed the IR-1 infrared spectrophotometer during World War II, and redesigned it as the IR-4 between 1953 and 1956.[2]:165[39]:6–7

Use

File:James Feeman using Beckman DU spectrophotometer 2004.012.021 crop.tif

The Beckman spectrophotometer was the first easy-to-use single instrument containing both the optical and electronic components needed for ultraviolet-absorption spectrophotometry within a single housing.[2]:153 The user could insert a cell tray with standard and sample cells, dial up the desired wavelength of light, confirm that the instrument was properly set by measuring the standard, and then measure the amount of absorption of the sample, reading the frequency from a simple meter.[40] A series of readings at different wavelengths could be taken without disturbing the sample.[41] The DU spectrophotometer's manual scanning method was extremely fast, reducing analysis times from weeks or hours to minutes.[39]:6 [42] [43]

It was accurate in both the visible and ultraviolet ranges.[29] Working in both the ultraviolet and the visible regions of the spectrum, the model DU produced accurate absorption spectra which could be obtained with relative ease and accurately replicated.[41] The National Bureau of Standards ran tests to certify that the DU's results were accurate and repeatable and recommended its use.[2]:156

Other advantages included its high resolution and the minimization of stray light in the ultraviolet region.[12] Although it was not cheap, its initial price of $723[12] made it available to the average laboratory.[44]:501 In comparison, in 1943, the GE Hardy Spectrophotometer cost $6,400.[39]:6 Practical and reliable, the DU rapidly established itself as a standard for laboratory equipment.[35]:141

Impact

File:Penicillin poster 5.40.tif

Credited with having "brought about a breakthrough in optical spectroscopy",[5]:10 the Beckman DU has been identified as "an indispensable tool for chemistry"[2]:207 and "the Model T of laboratory instruments".[12] Approximately 30,000 DU spectrophotometers were manufactured and sold between 1941 and 1976.[5]:11[45]

The DU enabled researchers to perform easier analysis of substances by quickly taking measurements at more than one wavelength to produce an absorption spectrum describing the complete substance. For example, the standard method of analysis of the vitamin A content of shark liver oil, before the introduction of the DU spectrophotometer, involved feeding the oil to rats for 21 days, then cutting off the rats' tails and examining their bone structure. With the DU's UV technology, vitamin A content of shark liver oil could be determined directly in a matter of minutes.[39]:6

The Scripps Research Institute and the Massachusetts Institute of Technology credit the DU with improving both accuracy and speed of chemical analysis. MIT states: "This device forever simplified and streamlined chemical analysis, by allowing researchers to perform a 99.9% accurate quantitative measurement of a substance within minutes, as opposed to the weeks required previously for results of only 25% accuracy."[42][43]

Inorganic chemist and philosopher of science Theodore L. Brown states that it "revolutionized the measurement of light signals from samples".[46]:2 Nobel laureate Bruce Merrifield is quoted as calling the DU spectrophotometer "probably the most important instrument ever developed towards the advancement of bioscience."[12] Historian of science Peter J. T. Morris identifies the introduction of the DU and other scientific instruments in the 1940s as the beginning of a Kuhnian revolution.[4] :80

For the Beckman company, the DU was one of three foundational inventions – the pH meter, the DU spectrophotometer, and the helipot potentiometer – that established the company on a secure financial basis and enabled it to expand.[47]

Vitamins

Development of the spectrophotometer had direct relevance to World War II and the American war effort. The role of vitamins in health was of significant concern, as scientists wanted to identify Vitamin A-rich foods to keep soldiers healthy. Previous methods of assessing Vitamin A levels involved feeding rats a food for several weeks and then performing a biopsy to estimate ingested Vitamin A levels. In contrast, examining a food sample with a DU spectrophotometer yielded better results in a matter of minutes.[48] The DU spectrophotometer could be used to study both vitamin A and its precursor carotenoids,[49] and rapidly became the preferred method of spectrophotometric analysis.[11][50][51]

Penicillin

The DU spectrophotometer was also an important tool for scientists studying and producing the new wonder drug penicillin.[10] The development of penicillin was a secret national mission, involving 17 drug companies, with the goal of providing penicillin to all U.S. Forces engaged in World War II.[52]:312[53] It was known that penicillin was more effective than sulfa drugs,[52]:312 and that its use reduced mortality, severity of long-term wound trauma, and recovery time.[2]:158 However, its structure was not understood, isolation procedures used to create pure cultures were primitive, and production using known surface culture techniques was slow.[52]:312

At Northern Regional Research Laboratory in Peoria, Illinois, researchers collected and examined more than 2,000 specimens of molds (as well as other microorganisms).[54] An extensive research team included Robert Coghill, Norman Heatley, Andrew Moyer, Mary Hunt,[55][56][57] Frank H. Stodola and Morris E. Friedkin. Friedkin recalls that an early model of the Beckman DU spectrophotometer was used by the penicillin researchers in Peoria.[52]:316 The Peoria lab was successful in isolating and commercially producing superior strains of the mold, which were 200 times more effective than the original forms discovered by Alexander Fleming.[55] By the end of the war, American pharmaceutical companies were producing 650 billion units of penicillin each month.[55] Much of the work done in this area during World War II was kept secret until after the war.[2]:158[53]

Hydrocarbons

The DU spectrophotometer was also used for critical analysis of hydrocarbons. A number of hydrocarbons were of interest to the war effort. Toluene, a hydrocarbon in crude oil, was used in production of TNT for military use.[2]:158–159[17]:19 Benzene and butadienes were used in the production of synthetic rubber.[58] Rubber, used in tires for jeeps, airplanes and tanks, was in critically short supply because the United States was cut off from foreign supplies of natural rubber.[2]:158–159 The Office of Rubber Reserve organized researchers at universities and in industry to secretly work on the problem.[59] The demand for synthetic rubber caused Beckman Instruments to develop infrared spectrophotometers. Infrared spectrophotometers were better suited than UV–Vis spectrophotometers to the analysis of C4 hydrocarbons, particularly for applications in petroleum refining and gasoline production.[2]:159[4]:17

Enzyme assays and DNA research

Gerty Cori and her husband Carl Ferdinand Cori won the Nobel Prize in Physiology or Medicine in 1947 in recognition of their work on enzymes. They made several discoveries critical to understanding carbohydrate metabolism, including the isolation and discovery of the Cori ester, glucose 1-phosphate, and the understanding of the Cori cycle. They determined that the enzyme phosphorylase catalyzes formation of glucose 1-phosphate, which is the beginning and ending step in the conversions of glycogen into glucose and blood glucose to glycogen. Gerty Cori was also the first to show that a defect in an enzyme can be the cause of a human genetic disease.[60] The Beckman DU spectrophotometer was used in the Cori laboratory to calculate enzyme concentrations, including phosphorylase.[61]

Another researcher who spent six months in 1947 at the Cori laboratory, "the most vibrant place in biochemistry" at that time, was Arthur Kornberg.[62] Kornberg was already familiar with the DU spectrophotometer, which he had used at Severo Ochoa's laboratory at New York University. The "new and scarce" Beckman DU, loaned to Ochoa by the American Philosophical Society, was highly prized and in constant use. Kornberg used it to purify aconitase, an enzyme in the citric acid cycle.[62][63]

"The enzyme could be assayed in a few minutes by coupling it to isocitrate dehydrogenase and in measuring the NADH formed using the Beckman DU spectrophotometer, an instrument that transformed biochemistry."[63]:113

Kornberg and Bernard L. Horecker used the Beckman DU spectrophotometer for enzyme assays measuring NADH and NADPH. They determined their extinction coefficients, establishing a basis for quantitative measurements in reactions involving nucleotides. This work became one of the most cited papers in biochemistry.[63]:115 Kornberg went on to study nucleotides in DNA synthesis, isolating the first DNA polymerizing enzyme (DNA polymerase I) in 1956 and receiving the Nobel Prize in Physiology or Medicine with Severo Ochoa in 1959.[64]

The bases of DNA absorbed ultraviolet light near 260 nm.[10] Inspired by the work of Oswald Avery[65] on DNA, Erwin Chargaff used a DU spectrophotometer in the 1940s in measuring the relative concentrations of bases in DNA.[66]:260, 290–302 Based on this research, he formulated Chargaff's rules.[67] In the first complete quantitative analysis of DNA, he reported the near-equal correspondence of pairs of bases in DNA, with the number of guanine units equaling the number of cytosine units, and the number of adenine units equaling the number of thymine units. He further demonstrated that the relative amounts of guanine, cytosine, adenine and thymine varied between species. In 1952, Chargaff met Francis Crick and James D. Watson, discussing his findings with them. Watson and Crick built upon his ideas in their determination of the structure of DNA.[67]

Biotechnology

Ultraviolet spectroscopy has wide applicability in molecular biology, particularly the study of photosynthesis.[68] It has been used to study a wide variety of flowering plants and ferns[69] by researchers in departments of biology, plant physiology and agriculture science as well as molecular genetics.[70]

Particularly useful in detecting conjugated double bonds, the new technology made it possible for researchers like Ralph Holman and George O. Burr to study dietary fats, work that had significant implications for human diet.[71] The DU spectrophotometer was also used in the study of steroids[72][73] by researchers like Alejandro Zaffaroni,[74] who helped to develop the birth control pill, the nicotine patch, and corticosteroids.[75]

Later models

File:Beckman DK1 Spectrophotometer 2002.003.004b.tif

The Beckman team eventually developed additional models, as well as a number of accessories or attachments which could be used to modify the DU for different types of work. One of the first accessories was a flame attachment with a more powerful photo multiplier to enable the user to examine flames such as potassium, sodium and cesium (1947).[16]:11[28]:230

In the 1950s, Beckman Instruments developed the DR and the DK, both of which were double-beam ultraviolet spectrophotometers. The DK was named for Wilbur I. Kaye, who developed it by modifying the DU to expand its range into the near-infrared.[16] He did the initial work while at Tennessee Eastman Kodak, and later was hired by Beckman Instruments.[76] The DKs introduced an automatic recording feature. The DK-1 used a non-linear scroll, and the DK-2 used a linear scroll to automatically record the spectra.[76]:21

The DR incorporated a "robot operator" which would reset the knobs on the DU to complete a sequence of measurements at different wavelengths, just like a human operator would to generate results for a full spectrum. It used a linear shuttle with four positions, and a superstructure to change the knobs. It had a moving chart recorder to plot results, with red, green and black dots.[16] The price of recording spectrophotometers was substantially higher than non-recording machines.[72]

The DK was ten times faster than the DR, but not quite as accurate.[16] It used a photomultiplier, which had introduced a source of error.[76]:21 The DK's speed made it preferred to the DR.[16] Kaye eventually developed the DKU, combining infrared and ultraviolet features in one instrument, but it was more expensive than other models.[76]

The last DU spectrophotometer was produced on July 6, 1976.[77] By the 1980s, computers were being incorporated into scientific instruments such as Bausch & Lomb's Spectronic 2000 UV–Vis spectrophotometer, to improve data acquisition and provide instrument control.[29] Specialized spectrophotometers designed for specific tasks now tend to be used rather than general "all-purpose machines" like the DU.[5]:1[78]

References

  1. ↑ Dulski, Thomas R. (1996). "The Wet Lab". A manual for the chemical analysis of metals (Online-Ausg. ed.). West Conshohocken, PA: ASTM. pp. 11–14. ISBN 978-0-8031-2066-2. https://books.google.com/books?id=ViOMjoLKB1gC&pg=PA11. Retrieved 30 August 2016. 
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 Arnold O. Beckman : one hundred years of excellence. Philadelphia, Pa.: Chemical Heritage Foundation. 2000. ISBN 978-0-941901-23-9. https://books.google.com/books?id=edtL_hIi8M0C&pg=PA150. 
  3. ↑ Braun, Robert Denton (2016). "Chemical analysis". Encyclopædia Britannica. https://www.britannica.com/science/chemical-analysis. Retrieved 30 August 2016. 
  4. ↑ 4.0 4.1 4.2 4.3 Morris, Peter J. T.; Travis, Anthony S. (2002). "The role of physical instrumentation in structural organic chemistry in the twentieth century". From classical to modern chemistry : the instrumental revolution. Cambridge: Royal Society of Chemistry. pp. 57–84. ISBN 978-0-85404-479-5. https://books.google.com/books?id=AAf8sk_v2SEC&pg=PA57. 
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Schmidt, Werner (2005). "Introduction to Optical Spectroscopy". Optical spectroscopy in chemistry and life sciences. Weinheim: Wiley-VCH. pp. 1–11. ISBN 978-3-527-29911-9. http://www.wiley-vch.de/books/sample/3527299114_c01.pdf. Retrieved 14 March 2016. 
  6. ↑ "Experiment 10". http://www.pa.msu.edu/courses/2013spring/PHY252/Lab10.pdf. 
  7. ↑ Harris, Daniel C.; Bertolucci, Michael D. (1989). Symmetry and spectroscopy : an introduction to vibrational and electronic spectroscopy (Reprint ed.). New York: Dover Publications. ISBN 978-0-486-66144-5. https://books.google.com/books?id=I3W6oSaRlMsC&pg=PA66. 
  8. ↑ Hollas, J. Michael (2004). Modern spectroscopy. Chichester: J. Wiley. ISBN 978-0-470-84416-8. https://archive.org/details/modernspectrosco0000holl_t7j3. 
  9. ↑ 9.0 9.1 Bigg, Charlotte (2002). "Adam Hilger, Ltd and the Development of Spectrochemical Analysis". From classical to modern chemistry : the instrumental revolution. Cambridge: Royal Society of Chemistry. pp. 111–128. ISBN 978-0-85404-479-5. https://books.google.com/books?id=AAf8sk_v2SEC&pg=PA113. 
  10. ↑ 10.0 10.1 10.2 10.3 10.4 10.5 Smutzer, Gregory (October 15, 2001). "Spectrophotometers: An Absorbing Tale". The Scientist. http://www.the-scientist.com/?articles.view/articleNo/13639/title/Spectrophotometers--An-Absorbing-Tale/. ""The DU spectrophotometer was extensively used in the mass production of penicillin."" 
  11. ↑ 11.0 11.1 11.2 Rosenfeld, Louis (1997). "Vitamine—vitamin. The early years of discovery". Clinical Chemistry 43 (4): 680–685. doi:10.1093/clinchem/43.4.680. PMID 9105273. http://www.clinchem.org/content/43/4/680.full. Retrieved 17 March 2016. ""The DU met a need and was an immediate success. It remained unsurpassed in its field for 35 years."". 
  12. ↑ 12.0 12.1 12.2 12.3 12.4 12.5 12.6 Simoni, Robert D.; Hill, Robert L.; Vaughan, Martha; Tabor, Herbert (December 5, 2003). "A Classic Instrument: The Beckman DU Spectrophotometer and Its Inventor, Arnold O. Beckman". Journal of Biological Chemistry 278 (e1): 79. doi:10.1016/S0021-9258(20)75750-9. http://www.jbc.org/content/278/49/e1.full.pdf. Retrieved 15 December 2015. 
  13. ↑ Gallwas, Jerry (2004). "People: Arnold Orville Beckman (1900–2004)". Analytical Chemistry 76 (15): 264 A–265 A. doi:10.1021/ac041608j. 
  14. ↑ Jaehnig, Kenton G.. Finding Aid to the Beckman historical collection, 1911–2011 (bulk 1934–2004). OCLC 899243886. https://othmerlib.sciencehistory.org/record=b1072260~S5. Retrieved 6 February 2018. ""Links on landing page go to full documents."" 
  15. ↑ Martelle, Scott (April 2, 2000). "During a Remarkable Century of Research, Innovation and Creative Tinkering, Visionary Arnold Beckman Seldom Found a Problem He Couldn't Solve. Until Now.". LA Times: p. 4. http://articles.latimes.com/2000/apr/02/magazine/tm-15008/4. 
  16. ↑ 16.0 16.1 16.2 16.3 16.4 16.5 16.6 Brock, David C.; Gallwas, Gerald E. (19 February 2002). Robert J. Manning, Transcript of an Interview Conducted by David C. Brock and Gerald E. Gallwas in Fullerton, California on 19 February 2002. Philadelphia, PA: Chemical Heritage Foundation. https://oh.sciencehistory.org/sites/default/files/manning_rj_0239_suppl.pdf. 
  17. ↑ 17.00 17.01 17.02 17.03 17.04 17.05 17.06 17.07 17.08 17.09 17.10 17.11 17.12 17.13 17.14 17.15 Belser, Karl Arnold. "Arnold Orville Beckman". http://www.karl-belser.com/History/Beckman_Arnold.pdf. 
  18. ↑ 18.0 18.1 Stewart, James E. (1996). Optical principles and technology for engineers. New York: M. Dekker. p. 202. ISBN 978-0-8247-9705-8. https://books.google.com/books?id=3INCfghbH48C&pg=PA202. Retrieved 9 September 2016. 
  19. ↑ Hunter, Richard S.; Harold, Richard W. (1987). The measurement of appearance (2nd ed.). New York: Wiley. pp. 246–248. ISBN 978-0-471-83006-1. https://books.google.com/books?id=vK5DK9vqyCgC&pg=PA246. 
  20. ↑ Bisen, Prakash S.; Sharma, Anjana (2013). Introduction to instrumentation in life sciences. Boca Raton, FL: CRC Press. p. 124. ISBN 978-1-4665-1240-5. https://books.google.com/books?id=T1HOBQAAQBAJ&pg=PA124. Retrieved 8 September 2016. 
  21. ↑ "TheSpectrophotometers That Would Not Die". http://calteches.library.caltech.edu/599/2/Spectrophotometers.pdf. 
  22. ↑ Glover, J. (1956). "Colorimetric, Absportimetric and Fluorimetric Methods". Modern Methods of Plant Analysis/Moderne Methoden der Pflanzenanalyse. Berlin: Springer-Verlag. pp. 149–245. ISBN 9783642805301. https://books.google.com/books?id=8KrrCAAAQBAJ&pg=PA184. Retrieved 30 August 2016. 
  23. ↑ Phillips, John P. (1964). Spectra-Structure Correlation. New York & London: Academic Press. p. 6. ISBN 9781483263557. https://books.google.com/books?id=BSMSBQAAQBAJ&pg=PA6. Retrieved 1 September 2016. 
  24. ↑ "Arthur H. Thomas Company". Analytical Chemistry 21 (2): 20A. February 1949. doi:10.1021/ac60026a720. 
  25. ↑ "Beckman Instruments". Analytical Chemistry 22 (12): 9A. December 1950. doi:10.1021/ac60048a708. 
  26. ↑ 26.0 26.1 26.2 26.3 26.4 Cary, H. H.; Beckman, Arnold O. (1941). "A Quartz Photoelectric Spectrophotometer". Journal of the Optical Society of America 31 (11): 682–689. doi:10.1364/JOSA.31.000682. https://www.osapublishing.org/josa/abstract.cfm?uri=josa-31-11-682. 
  27. ↑ 27.0 27.1 Robinson, James W. (1996). Atomic spectroscopy (2nd ed.). New York: Dekker. pp. 30–33. ISBN 978-0-8247-9742-3. https://books.google.com/books?id=BNqp0RO7DXcC&pg=PA30. Retrieved 9 September 2016. 
  28. ↑ 28.0 28.1 Gardiner, Kenneth (1956). "Flame photometry". in Berl, Walter G.. Physical Methods in Chemical Analysis (3 ed.). New York: Academic Press. pp. 219–290. ISBN 978-1-4832-5563-7. https://books.google.com/books?id=FSsXBQAAQBAJ&pg=PA230. Retrieved 17 March 2016. 
  29. ↑ 29.0 29.1 29.2 29.3 Buie, John (July 13, 2011). "Evolution of UV–Vis Spectrophotometers". pp. 24–25. http://www.sciglobalservices.com/pdfs/Evolution-JulAug-2011-25-26.pdf. 
  30. ↑ "Visible Proofs: Forensic Views of the Body". https://www.nlm.nih.gov/visibleproofs/galleries/technologies/spectral_image_5.html. 
  31. ↑ "Beckman Model DU Quartz Spectrophotometer". http://www.isl.uiuc.edu/Arnold_inventions/Spectrophotometer/Spectrophotometer.html. 
  32. ↑ Brode, Wallace R.; Gould, John H.; Whitney, James E.; Wyman, George M. (October 1953). "A Comparative Survey of Spectrophotometers in the 210–760 mμ Region". Journal of the Optical Society of America 43 (10): 862–865. doi:10.1364/JOSA.43.000862. Bibcode: 1953JOSA...43..862B. 
  33. ↑ Dulski, Thomas R. (1999). Trace elemental analysis of metals : methods and techniques. New York: Marcel Dekker. p. 195. ISBN 978-0-8247-1985-2. https://books.google.com/books?id=Vk7lTgTMEIEC&pg=PA195. Retrieved 31 August 2016. 
  34. ↑ Jarnutowski, R.; Ferraro, J. R.; Lankin, D. C. (1992). "Fifty years of commercial instrumentation in absorption spectroscopy, part II. Landmark instruments in UV/vis". Spectroscopy 7: 22–24, 26. 
  35. ↑ 35.0 35.1 Zweig, Gunter (1976). Analytical Methods for Pesticides, Plant Growth Regulators, and Food Additives: Principles, Methods, and General Applications. New York: Academic Press. pp. 141–143. ISBN 978-0-12-784301-8. https://books.google.com/books?id=oyPLBAAAQBAJ&pg=PA142. Retrieved 9 September 2016. 
  36. ↑ 36.00 36.01 36.02 36.03 36.04 36.05 36.06 36.07 36.08 36.09 36.10 Beckman Instruments Instruction Manual : The Beckman Model DU Spectrophotometer and Accessories. Fullerton, CA: Beckman Instruments, Inc.. 1954. 
  37. ↑ 37.0 37.1 37.2 "Announcing an Important New Beckman Development THE BECKMAN Spectrophotometer". News Edition of the American Chemical Society. American Chemical Society: p. NA. September 25, 1941. 
  38. ↑ Drees, Julia C.; Wu, Alan H. B. (2013). "Chapter 5: Analytic techniques". Clinical chemistry : principles, techniques, and correlations. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. pp. 131–134. ISBN 978-1-4511-1869-8. http://downloads.lww.com/wolterskluwer_vitalstream_com/sample-content/9780781790451_Bishop/samples/14460_Ch05.pdf. Retrieved 10 September 2016. 
  39. ↑ 39.0 39.1 39.2 39.3 Pillsbury, Dale (November 2011). "Too Bad Indy – You Were Born Too Early (Or Perhaps Arnold Beckman was Born Too Late)". The Alembic 38 (3): 6. http://www4.uwsp.edu/chemistry/acscws/38%20-%203%20November%202011.pdf. Retrieved 29 August 2016. ""conventional analysis for vitamin A content required analyzing the bone structure of the tails of rats fed oil for 21 days, while the Beckman DU with its UV capability was able to give a much more precise measurement of vitamin A content in just a few minutes."". 
  40. ↑ Harrison, George R.; Lord, Richard C.; Loofbourow, John R. (1948). Practical spectroscopy. New York: Prentice-Hall. pp. 400–402. https://babel.hathitrust.org/cgi/pt?id=uc1.b3740266;view=1up;seq=421. Retrieved 9 September 2016. 
  41. ↑ 41.0 41.1 "Beckman DU Spectrophotometer". National Museum of American History. http://americanhistory.si.edu/collections/search/object/nmah_399. 
  42. ↑ 42.0 42.1 "In Memoriam: Arnold O. Beckman (1900–2004)". News and Views 4 (18). May 24, 2004. https://www.scripps.edu/newsandviews/e_20040524/beckman.html. Retrieved 10 March 2016. ""The spectrophotometer improved biological assays from a process that took weeks and achieved 25 percent accuracy to one that took minutes and achieved 99.9 percent precision."". 
  43. ↑ 43.0 43.1 "Arnold O. Beckman". Massachusetts Institute of Technology. http://lemelson.mit.edu/resources/arnold-o-beckman. ""This device forever simplified and streamlined chemical analysis, by allowing researchers to perform a 99.9% accurate quantitative measurement of a substance within minutes, as opposed to the weeks required previously for results of only 25% accuracy."" 
  44. ↑ Royer, G. L.; Lawrence, H. C.; Kodama, S. P.; Warren, C. W. (April 1955). "Manual and Continuous Recording Attachments for Beckman Model DU Spectrophotometer". Analytical Chemistry 27 (4): 501–506. doi:10.1021/ac60100a005. 
  45. ↑ Browne, Malcolm W. (November 10, 1987). "After Decades, Inventor's Work Is Still Felt in the Laboratory". The New York Times. https://www.nytimes.com/1987/11/10/science/after-decades-inventor-s-work-is-still-felt-in-the-laboratory.html?pagewanted=all. 
  46. ↑ Brown, Theodore L. (2009). Bridging divides : the origins of the Beckman Institute at Illinois. Urbana: University of Illinois. ISBN 978-0-252-03484-8. 
  47. ↑ "Our History". https://www.beckmancoulter.com/wsrportal/wsr/company/about-us/our-history/index.htm. [yes|permanent dead link|dead link}}]
  48. ↑ Mark, Andrew (September 21, 2007). "Leading the way in chemical instrumentation". Laboratory News. http://www.labnews.co.uk/features/leading-the-way-in-chemical-instrumentation/. Retrieved 6 March 2014. 
  49. ↑ Avampato, J.E.; Eaton, H.D. (August 1953). "Comparison of Methods for the Estimation of Vitamin A in Calf Plasma". Journal of Dairy Science 36 (8): 783–793. doi:10.3168/jds.S0022-0302(53)91563-X. http://www.journalofdairyscience.org/article/S0022-0302(53)91563-X/abstract. Retrieved 10 September 2016. 
  50. ↑ Sebrell, W. H.; Harris, Robert S. (1954). The Vitamins: Chemistry, Physiology, Pathology. New York: Academic Press. p. 89. ISBN 9781483222028. https://books.google.com/books?id=KQjgBAAAQBAJ&pg=PA89. Retrieved 14 March 2016. ""... nowadays the preference is generally for the much easier and more precise measurement by a spectrophotometer, e.g. of the Beckman DU type ..."" 
  51. ↑ Cartwright, Anthony C. (June 28, 2015). The British Pharmacopoeia, 1864 to 2014: Medicines, International Standards and the State. Farnham, Surrey & Burlington, Vermont: Ashgate Publishing, Ltd.. ISBN 978-1-4724-2032-9. https://books.google.com/books?id=JvYjCQAAQBAJ&pg=PT239. Retrieved 17 March 2016. ""In 1941, they started selling the Beckman DU spectrophotometer which was a workhorse in countless laboratories for decades."" 
  52. ↑ 52.0 52.1 52.2 52.3 Friedkin, Morris E. (1995). "Growing up in the golden era of intermediary metabolism". Protein Science 4 (2): 311–325. doi:10.1002/pro.5560040218. PMID 7757020. ""The research was part of a national mission: to make penicillin available to all of our forces and by secrecy to prevent its use by Germany and Japan. Within a year, 17 drug companies were working on penicillin."". 
  53. ↑ 53.0 53.1 Sneader, Walter (2005). Drug Discovery: A History. Chichester: John Wiley. p. 294. ISBN 978-0-470-35929-7. https://books.google.com/books?id=Cb6BOkj9fK4C&pg=PA294. ""The drug exceeded all expectations, but the public were not told since penicillin was classified as a US military secret."" 
  54. ↑ Klaus, Abby. "Penicillin: "The Miracle Drug"". https://www.illinois.gov/ihpa/Research/IllinoisHistory/Documents/klaus106.pdf. 
  55. ↑ 55.0 55.1 55.2 Markel, Howard (2013-09-27). "The Real Story Behind Penicillin". September 27, 2013. PBS Newshour. https://www.pbs.org/newshour/rundown/the-real-story-behind-the-worlds-first-antibiotic/. 
  56. ↑ Kelly, Norman V. (April 20, 2013). "We called her 'Moldy Mary'". http://peoriahistorian.blogspot.com/2013/04/we-called-her-moldy-mary.html. 
  57. ↑ "Our View: Penicillin: Another bit of area history deserving notice". Journal Star. October 7, 2010. http://www.pjstar.com/article/20101007/NEWS/310079866. 
  58. ↑ Henglein, F. A.; Lang, R.F. (1968). Chemical technology (1st English ed.). Oxford: Pergamon Press. ISBN 978-0-08-011848-2. https://books.google.com/books?id=ie79BAAAQBAJ&pg=PA765. Retrieved 15 March 2016. 
  59. ↑ "Beckman Infrared Spectrometer". Chemical Heritage Foundation. http://www.chemheritage.org/research/policy-center/oral-history-program/projects/rubber-matters/feature-beckman-ir.aspx. 
  60. ↑ Smeltzer, Ronald K. (2013). Extraordinary Women in Science & Medicine: Four Centuries of Achievement. The Grolier Club. 
  61. ↑ Cori, Carl F.; Illingworth, Barbara (July 15, 1957). "The prosthetic group of phosphorylase". Proceedings of the National Academy of Sciences 43 (7): 547–552. doi:10.1073/pnas.43.7.547. PMID 16590054. Bibcode: 1957PNAS...43..547C. 
  62. ↑ 62.0 62.1 Kornberg, Arthur (January 5, 2001). "Remembering Our Teachers". The Journal of Biological Chemistry 276 (1): 3–11. doi:10.1016/S0021-9258(18)44198-1. PMID 11134064. http://www.jbc.org/content/276/1/3. Retrieved 17 March 2016. 
  63. ↑ 63.0 63.1 63.2 Exton, John H. (2013). "Arthur Kornberg". Crucible of science : the story of the Cori Laboratory. New York: Oxford University Press. pp. 112–122. ISBN 978-0-19-986107-1. https://books.google.com/books?id=4cdpAgAAQBAJ&pg=PA113. 
  64. ↑ Kresge, Nicole; Simoni, Robert D.; Hill, Robert L. (2005). "Arthur Kornberg's Discovery of DNA Polymerase I". J. Biol. Chem. 280 (46). http://www.jbc.org/cgi/content/full/280/49/e46. Retrieved 15 March 2016. 
  65. ↑ Avery, Oswald T.; MacLeod, Colin M.; McCarty, Maclyn (February 1, 1944). "Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types – Induction of Transformation by a Desoxyribonucleic Acid Fraction Isolated from Pneumococcus Type III". Journal of Experimental Medicine 79 (2): 137–158. doi:10.1084/jem.79.2.137. PMID 19871359. 
  66. ↑ Hunter, Graeme K. (2000). Vital forces the discovery of the molecular basis of life. San Diego: Academic Press. ISBN 978-0-12-361810-8. https://books.google.com/books?id=9bJex-NZd2MC&pg=PA290. Retrieved 17 March 2016. 
  67. ↑ 67.0 67.1 Board on Physics and Astronomy, Division on Engineering and Physical Sciences (2006). Instrumentation for a better tomorrow : proceedings of a symposium in honor of Arnold Beckman. Washington, D.C.: National Academies Press. p. 14. ISBN 978-0-309-10116-5. https://books.google.com/books?id=x8BVAgAAQBAJ&pg=PA14. 
  68. ↑ Sandage, Alan (2004). Centennial history of the Carnegie Institution of Washington.. 4. Cambridge: Cambridge University Press. p. 127. ISBN 978-0-521-83078-2. https://books.google.com/books?id=FAO-4gEjZbUC&pg=PA127. 
  69. ↑ Ludlow, C. Joseph; Wolf, Frederick T. (April 1975). "Photosynthesis and Respiration Rates of Ferns". American Fern Journal 65 (2): 43. doi:10.2307/1546309. 
  70. ↑ Sarkar, Sahotra (2001). The philosophy and history of molecular biology : new perspectives. Dordrecht: Kluwer Academic. p. 54. ISBN 978-1-4020-0249-6. https://books.google.com/books?id=605eE1hMW3kC&pg=PA54. Retrieved 17 March 2016. 
  71. ↑ Holman, Ralph T. (1997). "ω3 and ω6 Essential Fatty Acid Status in Human Health and Disease". Handbook of essential fatty acid biology biochemistry, physiology, and behavioral neurobiology. Totowa, N.J.: Humana Press. pp. 139–182. ISBN 978-1-4757-2582-7. https://books.google.com/books?id=omXmBwAAQBAJ&pg=PA139. Retrieved 17 March 2016. 
  72. ↑ 72.0 72.1 Morris, Peter J. T.; Travis, Anthony S. (2003). "Role of physical instrumentation in structural organic chemistry". Science in the twentieth century. London: Routledge. p. 725. ISBN 978-1-134-40686-9. https://books.google.com/books?id=ZYUfAgAAQBAJ&pg=PA725. Retrieved 14 March 2016. 
  73. ↑ Weisbart, Melvin (1973). Isolation and purification of hormones. New York: MSS Information Corp.. p. 47. ISBN 978-0-8422-7107-3. https://books.google.com/books?id=y0um1ya6UkYC&pg=PA48. Retrieved 17 March 2016. 
  74. ↑ Kornberg, Arthur (2002). The golden helix : inside biotech ventures. Sausalito, Calif.: University Science Books. p. 62. ISBN 978-1-891389-19-1. https://books.google.com/books?id=OO9gKGZmKCUC&pg=PA62. Retrieved 17 March 2016. 
  75. ↑ Pollack, Andrew (March 6, 2014). "Alejandro Zaffaroni, Entrepreneur on Biotech Frontier, Dies at 91". The New York Times. https://www.nytimes.com/2014/03/07/business/alejandro-zaffaroni-biotechnology-entrepreneur-dies-at-91.html?_r=0. 
  76. ↑ 76.0 76.1 76.2 76.3 Thackray, Arnold; Gallwas, Gerald E. (27 February 2002). Wilbur I. Kaye, Transcript of an Interview Conducted by Arnold Thackray and Gerald E. Gallwas at La Jolla, California on 11 and 27 February 2002. Philadelphia, PA: Chemical Heritage Foundation. https://oh.sciencehistory.org/sites/default/files/kaye_w_0232_suppl.pdf. 
  77. ↑ Beckman, A. O.; Gallaway, W. S.; Kaye, W.; Ulrich, W. F. (March 1977). "History of spectrophotometry at Beckman Instruments, Inc". Analytical Chemistry 49 (3): 280A–300A. doi:10.1021/ac50011a001. 
  78. ↑ Beck, Shane (February 2, 1998). "Across the Spectrum: Instrumentation for UV/Vis Spectrophotometry". The Scientist. http://www.the-scientist.com/?articles.view/articleNo/18797/title/Across-the-Spectrum--Instrumentation-for-UV-Vis-Spectrophotometry/. Retrieved 9 September 2016. 

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