Biology:List of restriction enzyme cutting sites: Bst–Bv
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Revision as of 04:55, 14 August 2022 by imported>Wincert (change)
Short description: None
Legend of nucleobases | |
---|---|
Code | Nucleotide represented |
A | Adenine (A) |
C | Cytosine (C) |
G | Guanine (G) |
T | Thymine (T) |
N | A, C, G or T |
M | A or C |
R | A or G |
W | A or T |
Y | C or T |
S | C or G |
K | G or T |
H | A, C or T |
B | C, G or T |
V | A, C or G |
D | A, G or T |
This article contains a list of the most studied restriction enzymes whose names start with Bst to Bv inclusive. It contains approximately 200 enzymes.
The following information is given:
- Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
- PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
- Source: Organism that naturally produces the enzyme.
- Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
- Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
- Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme: EcoR70I
Restriction enzymes
Bst
Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers | ||||||
BstI | Bacillus stearothermophilus 1503-4R | 5' GGATCC 3' CCTAGG
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5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
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AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, Nsp29132II | |||||||
Bst1I | Bacillus stearothermophilus 1 | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AglI, BseBI, Bse17I, BstNI, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXI | |||||||
Bst2I | Bacillus stearothermophilus 2 | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AglI, BseBI, Bse17I, BstNI, BstOI, Bst2UI, CthII, EcoRII, MvaI | |||||||
Bst6I | Bacillus stearothermophilus 6 | 5' CTCTTC 3' GAGAAG
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5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5'
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Bst11I | Bacillus stearothermophilus 11 | 5' ACTGG 3' TGACC
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5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5'
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Bst12I | Bacillus stearothermophilus 12 | 5' GCAGC 3' CGTCG
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5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5'
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AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst71I, BstV1I | |||||||
Bst19I | Bacillus stearothermophilus 19 | 5' GCATC 3' CGTAG
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5' ---GCATCNNNN NN--- 3' 3' ---CGTAGNNNNNN --- 5'
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Bst19II | Bacillus stearothermophilus 19 | 5' GATC 3' CTAG
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5' --- GATC--- 3' 3' ---CTAG --- 5'
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BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI | |||||||
Bst28I | Bacillus stearothermophilus 28 | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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BciBI, BdiI, Bli41I, Bsa29I, Bsp106I, BspDI, LplI, SpmI, ZhoI | |||||||
Bst38I | Bacillus stearothermophilus 38 | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AorI, BseBI, Bse17I, BsiLI, BspNI, BstOI, Bst2UI, EcoRII, MvaI | |||||||
Bst40I | Bacillus stearothermophilus 40 | 5' CCGG 3' GGCC
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5' ---C CGG--- 3' 3' ---GGC C--- 5'
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Bst71I | Bacillus stearothermophilus 71 | 5' GCAGC 3' CGTCG
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5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5'
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AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, BstV1I | |||||||
Bst98I | Bacillus stearothermophilus C98 | 5' CTTAAG 3' GAATTC
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5' ---C TTAAG--- 3' 3' ---GAATT C--- 5'
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Bst100I | Bacillus stearothermophilus 100 | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI | |||||||
Bst1107I | Bacillus stearothermophilus RFL1107 | 5' GTATAC 3' CATATG
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5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5'
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BstACI | Bacillus stearothermophilus AC | 5' GRCGYC 3' CYGCRG
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5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5'
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AcyI, AhaII, AosII, AstWI, AsuIII, BsaHI, HgiI, HgiDI, Hsp92I | |||||||
BstAPI | Bacillus stearothermophilus AP | 5' GCAN4NTGC 3' CGTN4NACG
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5' ---GCANNNN NTGC--- 3' 3' ---CGTN NNNNACG--- 5'
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ApaBI | |||||||
BstAUI | Bacillus stearothermophilus AU | 5' TGTACA 3' ACATGT
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5' ---T GTACA--- 3' 3' ---ACATG T--- 5'
|
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BstBI | Bacillus stearothermophilus B225 | 5' TTCGAA 3' AAGCTT
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5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
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AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, LspI, NspV, SspRFI | |||||||
Bst2BI | Bacillus stearothermophilus 2B | 5' CACGAG 3' GTGCTC
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5' ---C ACGAG--- 3' 3' ---GTGCT C--- 5'
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BstBAI | Bacillus stearothermophilus BA | 5' YACGTR 3' RTGCAY
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5' ---YAC GTR--- 3' 3' ---RTG CAY--- 5'
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BstBSI | Bacillus stearothermophilus BS | 5' GTATAC 3' CATATG
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5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5'
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BstB7SI | Bacillus stearothermophilus B7S | 5' RCCGGY 3' YGGCCR
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5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5'
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BstBS32I | Bacillus stearothermophilus BS32 | 5' GAAGAC 3' CTTCTG
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5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
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BstBZ153I | Bacillus stearothermophilus BZ153 | 5' GCGCGC 3' CGCGCG
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5' ---G CGCGC--- 3' 3' ---CGCGC G--- 5'
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Bst4CI | Bacillus stearothermophilus 4C | 5' ACNGT 3' TGNCA
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5' ---ACN GT--- 3' 3' ---TG NCA--- 5'
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BstC8I | Bacillus stearothermophilus C8 | 5' GCNNGC 3' CGNNCG
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5' ---GCN NGC--- 3' 3' ---CGN NCG--- 5'
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BstD102I | Bacillus stearothermophilus D102 | 5' CCGCTC 3' GGCGAG
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5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5'
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AccBSI, BsrBI, Bst31NI, MbiI | |||||||
BstDEI | Bacillus stearothermophilus DE | 5' CTNAG 3' GANTC
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5' ---C TNAG--- 3' 3' ---GANT C--- 5'
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BstDSI | Bacillus stearothermophilus DS | 5' CCRYGG 3' GGYRCC
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5' ---C CRYGG--- 3' 3' ---GGYRC C--- 5'
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BstEII | Bacillus stearothermophilus ET | 5' GGTNACC 3' CCANTGG
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5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
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AcrII, BstPI, BstT9I, BstT10I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI | |||||||
BstENI | Bacillus stearothermophilus EN | 5' CCTN4NAGG 3' GGAN4NTCC
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5' ---CCTNN NNNAGG--- 3' 3' ---GGANNN NNTCC--- 5'
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BstENII | Bacillus stearothermophilus EN | 5' GATC 3' CTAG
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5' --- GATC--- 3' 3' ---CTAG --- 5'
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BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI | |||||||
BstEZ359I | Bacillus stearothermophilus EZ359 | 5' GTTAAC 3' CAATTG
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5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5'
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BstFI | Bacillus stearothermophilus FH58 | 5' AAGCTT 3' TTCGAA
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5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5'
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BstF5I | Bacillus stearothermophilus F5 | 5' GGATG 3' CCTAC
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5' ---GGATGNN --- 3' 3' ---CCTAC NN--- 5'
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BstFNI | Bacillus stearothermophilus | 5' CGCG 3' GCGC
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5' ---CG CG--- 3' 3' ---GC GC--- 5'
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AccII, BceBI, BepI, Bpu95I, BstUI, Csp68KVI, FauBII, MvnI, SelI | |||||||
BstFZ438I | Bacillus stearothermophilus FZ438 | 5' CCCGC 3' GGGCG
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5' ---CCCGCNNNN NN--- 3' 3' ---GGGCGNNNNNN --- 5'
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BstGZ53I | Bacillus stearothermophilus GZ53 | 5' CGTCTC 3' GCAGAG
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5' ---CGTCTCN NNNN--- 3' 3' ---GCAGAGNNNNN --- 5'
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BstH2I | Bacillus stearothermophilus H2 | 5' RGCGCY 3' YCGCGR
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5' ---RGCGC Y--- 3' 3' ---Y CGCGR--- 5'
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BstH9I | Bacillus stearothermophilus H9 | 5' GGATC 3' CCTAG
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5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5'
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BstHHI | Bacillus stearothermophilus HH | 5' GCGC 3' CGCG
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5' ---GCG C--- 3' 3' ---C GCG--- 5'
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AspLEI, BspLAI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI | |||||||
BstHPI | Bacillus stearothermophilus HP | 5' GTTAAC 3' CAATTG
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5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5'
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BstHZ55I | Bacillus stearothermophilus HZ55 | 5' CCAN6TGG 3' GGTN6ACC
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5' ---CCANNNNN NTGG--- 3' 3' ---GGTN NNNNNACC--- 5'
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BstIZ316I | Bacillus stearothermophilus IZ316 | 5' CACNNNGTG 3' GTGNNNCAC
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5' ---CACNNN GTG--- 3' 3' ---GTG NNNCAC--- 5'
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AdeI, DraIII | |||||||
BstJZ301I | Bacillus stearothermophilus JZ301 | 5' CTNAG 3' GANTC
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5' ---C TNAG--- 3' 3' ---GANT C--- 5'
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BstKTI | Bacillus stearothermophilus KT | 5' GATC 3' CTAG
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5' ---GAT C--- 3' 3' ---C TAG--- 5'
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AspMDI, Bsp2095I, Bst19II, BspKT6I, DpnII, MboI, NmeCI, SsiAI | |||||||
BstM6I | Bacillus stearothermophilus M6 | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, Fsp1604I, MvaI | |||||||
BstMAI | Bacillus stearothermophilus MA | 5' GTCTC 3' CAGAG
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5' ---GTCTCN NNNNN--- 3' 3' ---CAGAGNNNNN N--- 5'
|
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BstMBI | Bacillus stearothermophilus MB | 5' GATC 3' CTAG
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5' --- GATC--- 3' 3' ---CTAG --- 5'
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Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I | |||||||
BstMCI | Bacillus stearothermophilus MC | 5' CGRYCG 3' GCYRGC
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5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5'
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BstMWI | Bacillus stearothermophilus MW | 5' GCN7GC 3' CGN7CG
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5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5'
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BstMZ611I | Bacillus stearothermophilus MZ611 | 5' CCNGG 3' GGNCC
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5' --- CCNGG--- 3' 3' ---GGNCC --- 5'
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BstNI | Bacillus stearothermophilus | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AjnI, BciBII, BptI, Bst1I, BstOI, Bst2UI, Fsp1604I, SniI, Sth117I | |||||||
Bst31NI | Bacillus stearothermophilus 31N | 5' CCGCTC 3' GGCGAG
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5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5'
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AccBSI, BsrBI, BstD102I, MbiI | |||||||
BstNSI | Bacillus stearothermophilus 1161NS | 5' RCATGY 3' YGTACR
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5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5'
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BstNZ169I | Bacillus stearothermophilus NZ169 | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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BanIII, BciBI, BdiI, Bli41I, Bsa29I, BspDI, LplI, Rme21I, SpmI, ZhoI | |||||||
BstOI | Bacillus stearothermophilus O22 | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AjnI, ApyI, BciBII, BptI, Bst1I, Bst2UI, Fsp1604I, SniI, Sth117I | |||||||
BstOZ616I | Bacillus stearothermophilus OZ616 | 5' GGGAC 3' CCCTG
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5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5'
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BstPI | Bacillus stearothermophilus | 5' GGTNACC 3' CCANTGG
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5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
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AcrII, AspAI, BstEII, BstT9I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI | |||||||
BstPAI | Bacillus stearothermophilus PA | 5' GACN4GTC 3' CTGN4CAG
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5' ---GACNN NNGTC--- 3' 3' ---CTGNN NNCAG--- 5'
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BstPZ418I | 5' GGATG 3' CCTAC
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5' ---GGATGN8N NNNN--- 3' 3' ---CCTACN8NNNNN --- 5'
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BstPZ740I | Bacillus stearothermophilus PZ740 | 5' CTTAAG 3' GAATTC
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5' ---C TTAAG--- 3' 3' ---GAATT C--- 5'
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BstRZ246I | Bacillus stearothermophilus RZ246 | 5' ATTTAAAT 3' TAAATTTA
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5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5'
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BstSI | Bacillus stearothermophilus S183 | 5' CYCGRG 3' GRGCYC
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5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5'
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Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, NspIII, OfoI, PunAI | |||||||
BstSCI | Bacillus stearothermophilus SC | 5' CCNGG 3' GGNCC
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5' --- CCNGG--- 3' 3' ---GGNCC --- 5'
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BstSFI | Bacillus stearothermophilus SF | 5' CTRYAG 3' GAYRTC
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5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5'
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BstSNI | Bacillus stearothermophilus SN | 5' TACGTA 3' ATGCAT
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5' ---TAC GTA--- 3' 3' ---ATG CAT--- 5'
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BstSWI | Bacillus stearothermophilus SW | 5' ATTTAAAT 3' TAAATTTA
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5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5'
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BstT7I | Bacillus stearothermophilus T7 | 5' TGATCA 3' ACTAGT
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5' ---T GATCA--- 3' 3' ---ACTAG T--- 5'
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BstT9I | Bacillus stearothermophilus T9 | 5' GGTNACC 3' CCANTGG
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5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
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AspAI, Bse64I, BstT10I, BstPI, Eco91I, EcoO128I, NspSAII, PspEI | |||||||
BstT10I | Bacillus stearothermophilus T10 | 5' GGTNACC 3' CCANTGG
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5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
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AspAI, BseT10I, BstEII, BstT10I, Eco91I, EcoO128I, NspSAII, PspEI | |||||||
Bst31TI | Bacillus stearothermophilus 31T | 5' GGATC 3' CCTAG
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5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5'
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BstTS5I | Bacillus stearothermophilus TS5 | 5' GAAGAC 3' CTTCTG
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5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
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BstUI | Bacillus stearothermophilus U458 | 5' CGCG 3' GCGC
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5' ---CG CG--- 3' 3' ---GC GC--- 5'
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AccII, BceBI, BepI, Bpu95I, BtkI, Csp68KVI, FauBII, MvnI, SelI | |||||||
Bst2UI | Bacillus stearothermophilus 2U | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, SniI, SslI, TaqXI, ZanI | |||||||
BstVI | Bacillus stearothermophilus V | 5' CTCGAG 3' GAGCTC
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5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
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BluI, BssHI, MlaAI, PanI, SauLPII, SciI, SlaI, StrI, XhoI | |||||||
BstV1I | Bacillus stearothermophilus V1 | 5' GCAGC 3' CGTCG
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5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5'
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AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I | |||||||
BstV2I | Bacillus stearothermophilus V2 | 5' GAAGAC 3' CTTCTG
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5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
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BstXI | Bacillus stearothermophilus X1 | 5' CCAN6TGG 3' GGTN6ACC
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5' ---CCANNNNN NTGG--- 3' 3' ---GGTN NNNNNACC--- 5'
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BstX2I | Bacillus stearothermophilus X2 | 5' RGATCY 3' YCTAGR
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5' ---R GATCY--- 3' 3' ---YCTAG R--- 5'
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BstYI | 1SDO | Bacillus stearothermophilus Y406 | 5' RGATCY 3' YCTAGR
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5' ---R GATCY--- 3' 3' ---YCTAG R--- 5'
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BstZI | Bacillus stearothermophilus Z130 | 5' CGGCCG 3' GCCGGC
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5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5'
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AaaI, BseX3I, EagI, EclXI, Eco52I, SenPT16I, XmaIII | |||||||
BstZ17I | Bacillus stearothermophilus 38M | 5' GTATAC 3' CATATG
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5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5'
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Bsu - Bv
Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers |
Bsu6I | Bacillus subtilis 6v1 | 5' CTCTTC 3' GAGAAG
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5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5'
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Bsu15I | Bacillus subtilis 15 | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, BsuTUI, ClaI | |
Bsu23I | Bacillus subtilis 23 | 5' TCCGGA 3' AGGCCT
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5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5'
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AccIII, BbvAIII, BseAI, BsiMI, BspEI, BspMII, CauB3I, MroI | |
Bsu36I | Bacillus subtilis 36 | 5' CCTNAGG 3' GGANTCC
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5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5'
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AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI | |
Bsu54I | Bacillus subtilis 54 | 5' GGNCC 3' CCNGG
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5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
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AsuI, Bac36I, BavBII, BsiZI, BspBII, CcuI, MaeK81II, PspPI | |
Bsu1532I | Bacillus subtilis 1532 | 5' CGCG 3' GCGC
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5' ---CG CG--- 3' 3' ---GC GC--- 5'
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BceBI, Bpu95I, Bsp123I, BstUI, BtkI, Csp68KVI, FnuDII, MvnI, ThaI | |
Bsu1854I | Bacillus subtilis 1854 | 5' GRGCYC 3' CYCGRG
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5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
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BsuBI | Bacillus subtilis | 5' CTGCAG 3' GACGTC
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5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
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AjoI, BloHII, CflI, CstI, Ecl37kI, HalII, Psp23I, PstI, SalPI, SflI, YenI | |
BsuFI | Bacillus subtilis | 5' CCGG 3' GGCC
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5' ---C CGG--- 3' 3' ---GGC C--- 5'
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BsuMI | Bacillus subtilis 168 | 5' CTCGAG 3' GAGCTC
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5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
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XhoI, BssHI, TliI, PaeR7I, Sfr274I, SlaI, StrI | |
BsuRI | Bacillus subtilis R | 5' GGCC 3' CCGG
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5' ---GG CC--- 3' 3' ---CC GG--- 5'
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HaeIII | |
BsuTUI | Bacillus subtilis TU | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, ClaI | |
BteI | Brochothrix thermosphacter | 5' GGCC 3' CCGG
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5' ---GG CC--- 3' 3' ---CC GG--- 5'
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BtgI | Bacillus thermoglucosidasius | 5' CCRYGG 3' GGYRCC
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5' ---C CRYGG--- 3' 3' ---GGYRC C--- 5'
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BtgZI | Bacillus thermoglucosidasius | 5' GCGATG 3' CGCTAC
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5' ---GCGATGN8NN NNNN--- 3' 3' ---CGCTACN8NNNNNN --- 5'
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BthAI | Bacillus thuringiensis AII | 5' GGWCC 3' CCWGG
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5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
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BamNxI, BcuAI, BsrAI, Eco47I, FspMSI, HgiCII, HgiJI, SmuEI | |
BthCI | Bacillus thuringiensis 2294 | 5' GCNGC 3' CGNCG
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5' ---GCNG C--- 3' 3' ---C GNCG--- 5'
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BthDI | Bacillus thuringiensis D4 | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AorI, ApyI, Bse24I, BstNI, BstOI, Bst2UI, CbrI, EcoRII, MvaI, SleI | |
BthEI | Bacillus thuringiensis E | 5' CCWGG 3' GGWCC
|
5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
|
AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI | |
BtkI | Bacillus thuringiensis Kumantoensis | 5' CGCG 3' GCGC
|
5' ---CG CG--- 3' 3' ---GC GC--- 5'
|
BepI, Bpu95I, Bsp123I, BstFNI, BstUI, BtkI, FalII, SelI, ThaI | |
BtkII | Bacillus thuringiensis Kumantoensis | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
BfuCI, Bsp67I, Bst19II, CcyI, FnuCI, MboI, NmeCI, SauMI | |
BtrI | Bacillus stearothermophilus SE-U62 | 5' CACGTC 3' GTGCAG
|
5' ---CAC GTC--- 3' 3' ---GTG CAG--- 5'
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BtsI | Bacillus thermoglucosidasius | 5' GCAGTG 3' CGTCAC
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5' ---GCAGTGNN --- 3' 3' ---CGTCAC NN--- 5'
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BveI | Brevundimonas vesicularis RFL1 | 5' ACCTGC 3' TGGACG
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5' ---ACCTGCNNNN NNNN--- 3' 3' ---TGGACGNNNNNNNN --- 5'
|
Acc36I, BfuAI, BspMI | |
BvuI | Bacillus vulgatis | 5' GRGCYC 3' CYCGRG
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5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
|
||
BvuBI | Bacteroides vulgatus | 5' CGTACG 3' GCATGC
|
5' ---C GTACG--- 3' 3' ---GCATG C--- 5'
|
Notes
- ↑ "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. December 1973. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
- ↑ "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. April 2003. doi:10.1093/nar/gkg274. PMID 12654995.
- ↑ "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. 2002. ISBN 0-7167-4684-0. https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=books&doptcmdl=GenBookHL&term=stryer%5Bbook%5D+AND+215168%5Buid%5D&rid=stryer.chapter.280.
- ↑ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
- ↑ "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene 92 (1-2): 1–248. August 1990. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
- ↑ "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. September 2001. doi:10.1093/nar/29.18.3705. PMID 11557805.
![]() | Original source: https://en.wikipedia.org/wiki/List of restriction enzyme cutting sites: Bst–Bv.
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