Biology:Pyrrolysine—tRNAPyl ligase

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Pyrrolysine—tRNAPyl ligase
Identifiers
EC number6.1.1.26
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Pyrrolysine—tRNAPyl ligase (EC 6.1.1.26, PylS, pyrrolysyl-tRNA synthetase) is an enzyme with systematic name L-pyrrolysine:tRNAPyl ligase (AMP-forming).[1][2][3] This enzyme catalyses the following chemical reaction

ATP + L-pyrrolysine + tRNAPyl [math]\displaystyle{ \rightleftharpoons }[/math] AMP + diphosphate + L-pyrrolysyl-tRNAPyl

This enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids.

References

  1. "Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo". Nature 431 (7006): 333–5. September 2004. doi:10.1038/nature02895. PMID 15329732. 
  2. "An aminoacyl-tRNA synthetase that specifically activates pyrrolysine". Proceedings of the National Academy of Sciences of the United States of America 101 (34): 12450–4. August 2004. doi:10.1073/pnas.0405362101. PMID 15314242. 
  3. "Molecular biology: genetic code seizes pyrrolysine". Nature 431 (7006): 257–8. September 2004. doi:10.1038/431257a. PMID 15372017. 

External links