Biology:BRCC3
 Generic protein structure example |
Lys-63-specific deubiquitinase BRCC36 is an enzyme that in humans is encoded by the BRCC3 gene.[1][2][3]
Function
This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint. Alternative splicing results in multiple transcript variants.[3]
Repair of DNA damage
BRCC36, the protein product of the BRCC3 gene, is a deubiquitinating enzyme and a core component of the deubiquitin complex BRCA1-A.[4] BRCA1, as distinct from BRCA1-A, is employed in the repair of chromosomal damage with an important role in the error-free homologous recombinational (HR) repair of DNA double-strand breaks. Sequestration of BRCA1 away from the DNA damage site suppresses homologous recombination and redirects the cell in the direction of repair by the process of non-homologous end joining (NHEJ).[4] The role of BRCA1-A appears to be to bind BRCA1 with high affinity and withdraw it away from the site of DNA damage to the periphery where it remains sequestered, thus promoting DNA repair by NHEJ in preference to HR.
Interactions
BRCC3 has been shown to interact with BRE,[2][5] BRCA2,[2] RAD51,[2] BRCA1,[2] P53[2] and BARD1.[2]
References
- ↑ "Isolation and sequence of two genes associated with a CpG island 5' of the factor VIII gene". Human Molecular Genetics 1 (3): 179–86. Jun 1992. doi:10.1093/hmg/1.3.179. PMID 1303175.
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 "Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair". Molecular Cell 12 (5): 1087–99. Nov 2003. doi:10.1016/S1097-2765(03)00424-6. PMID 14636569.
- ↑ 3.0 3.1 "Entrez Gene: BRCC3 BRCA1/BRCA2-containing complex, subunit 3". https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=79184.
- ↑ 4.0 4.1 Rabl, J. (2020). "BRCA1-A and BRISC: Multifunctional Molecular Machines for Ubiquitin Signaling". Biomolecules 10 (11): 1503. doi:10.3390/biom10111503. PMID 33142801.
- ↑ "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1). 2007. doi:10.1038/msb4100134. PMID 17353931.
External links
- Human BRCC3 genome location and BRCC3 gene details page in the UCSC Genome Browser.
Further reading
- "The chromosomal translocation t(X;14)(q28;q11) in T-cell pro-lymphocytic leukaemia breaks within one gene and activates another". Oncogene 8 (12): 3271–6. Dec 1993. PMID 8247530.
- "DNA cloning using in vitro site-specific recombination". Genome Research 10 (11): 1788–95. Nov 2000. doi:10.1101/gr.143000. PMID 11076863.
- "JAMM: a metalloprotease-like zinc site in the proteasome and signalosome". PLOS Biology 2 (1): E2. Jan 2004. doi:10.1371/journal.pbio.0020002. PMID 14737182.
- "From ORFeome to biology: a functional genomics pipeline". Genome Research 14 (10B): 2136–44. Oct 2004. doi:10.1101/gr.2576704. PMID 15489336.
- "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. Oct 2005. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R.
- "The LIFEdb database in 2006". Nucleic Acids Research 34 (Database issue): D415–8. Jan 2006. doi:10.1093/nar/gkj139. PMID 16381901.
- "Expression of BRCC3, a novel cell cycle regulated molecule, is associated with increased phospho-ERK and cell proliferation". International Journal of Molecular Medicine 19 (1): 29–39. Jan 2007. doi:10.3892/ijmm.19.1.29. PMID 17143545.
- "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1). 2007. doi:10.1038/msb4100134. PMID 17353931.
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