Biology:Cell cortex

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Short description: Layer on the inner face of a cell membrane
F-actin distribution in the cell cortex as shown by rhodamine phalloidin staining of HeLa cells that constitutively express Histone H2B-GFP to mark chromosomes. F-actin is thus red, while Histone H2B is displayed in green. The left hand cell is in mitosis, as demonstrated by chromosome condensation, while the right hand cell is in interphase(as determined by intact cell nucleus) in a suspended state. In both cases F-actin is enriched around the cell periphery. Scale bar: 10 micrometers.

The cell cortex, also known as the actin cortex, cortical cytoskeleton or actomyosin cortex, is a specialized layer of cytoplasmic proteins on the inner face of the cell membrane. It functions as a modulator of membrane behavior and cell surface properties.[1][2][3] In most eukaryotic cells lacking a cell wall, the cortex is an actin-rich network consisting of F-actin filaments, myosin motors, and actin-binding proteins.[4][5] The actomyosin cortex is attached to the cell membrane via membrane-anchoring proteins called ERM proteins that plays a central role in cell shape control.[1][6] The protein constituents of the cortex undergo rapid turnover, making the cortex both mechanically rigid and highly plastic, two properties essential to its function. In most cases, the cortex is in the range of 100 to 1000 nanometers thick.

In some animal cells, the protein spectrin may be present in the cortex. Spectrin helps to create a network by cross-linked actin filaments.[3] The proportions of spectrin and actin vary with cell type.[7] Spectrin proteins and actin microfilaments are attached to transmembrane proteins by attachment proteins between them and the transmembrane proteins. The cell cortex is attached to the inner cytosolic face of the plasma membrane in cells where the spectrin proteins and actin microfilaments form a mesh-like structure that is continuously remodeled by polymerization, depolymerization and branching.

Many proteins are involved in the cortex regulation and dynamics including formins with roles in actin polymerization, Arp2/3 complexes that give rise to actin branching and capping proteins. Due to the branching process and the density of the actin cortex, the cortical cytoskeleton can form a highly complex meshwork such as a fractal structure.[8] Specialized cells are usually characterized by a very specific cortical actin cytoskeleton. For example, in red blood cells, the cell cortex consists of a two-dimensional cross-linked elastic network with pentagonal or hexagonal symmetry, tethered to the plasma membrane and formed primarily by spectrin, actin and ankyrin.[9] In neuronal axons the actin/spectric cytoskeleton forms an array of periodic rings [10] and in the sperm flagellum it forms a helical structure.[11]

In plant cells, the cell cortex is reinforced by cortical microtubules underlying the plasma membrane. The direction of these cortical microtubules determines which way the cell elongates when it grows.

Functions

The cortex mainly functions to produce tension under the cell membrane, allowing the cell to change shape.[12] This is primarily accomplished through myosin II motors, which pull on the filaments to generate stress.[12] These changes in tension are required for the cell to change its shape as it undergoes cell migration and cell division.[12]

In mitosis, F-actin and myosin II form a highly contractile and uniform cortex to drive mitotic cell rounding. The surface tension produced by the actomyosin cortex activity generates intracellular hydrostatic pressure capable of displacing surrounding objects to facilitate rounding.[13][14] Thus, the cell cortex serves to protect the microtubule spindle from external mechanical disruption during mitosis.[15] When external forces are applied at sufficiently large rate and magnitude to a mitotic cell, loss of cortical F-actin homogeneity occurs leading to herniation of blebs and a temporary loss of the ability to protect the mitotic spindle.[16] Genetic studies have shown that the cell cortex in mitosis is regulated by diverse genes such as Rhoa,[17] WDR1,[18] ERM proteins,[19] Ect2,[20] Pbl, Cdc42, aPKC, Par6,[21] DJ-1 and FAM134A.[22]

In cytokinesis the cell cortex plays a central role by producing a myosin-rich contractile ring to constrict the dividing cell into two daughter cells.[23]

Cell cortex contractility is key for amoeboidal type cell migration characteristic of many cancer cell metastasis events.[1][24]

Research

Basic research into the cell cortex is done with immortalised cell lines, typically HeLa cells, S2 cells, Normal rat kidney cells, and M2 cells.[12] In M2 cells in particular, cellular blebs – which form without a cortex, then form one as they retract – are often used to model cortex formation and composition.[12]

References

  1. ↑ 1.0 1.1 1.2 "Actin cortex mechanics and cellular morphogenesis". Trends in Cell Biology 22 (10): 536–45. October 2012. doi:10.1016/j.tcb.2012.07.001. PMID 22871642. 
  2. ↑ "Micromechanical architecture of the endothelial cell cortex". Biophysical Journal 88 (1): 670–9. January 2005. doi:10.1529/biophysj.104.049965. PMID 15489304. Bibcode: 2005BpJ....88..670P. 
  3. ↑ 3.0 3.1 Alberts, Bruce; Johnson, Alexander; Lewis, Julian; Raff, Martin; Roberts, Keith; Walter, Peter (2002). "Cross-linking Proteins with Distinct Properties Organize Different Assemblies of Actin Filaments". Molecular Biology of the Cell (4th ed.). New York: Garland Science. ISBN 0-8153-3218-1. https://www.ncbi.nlm.nih.gov/books/NBK26809/#A3021. 
  4. ↑ "The evolution of compositionally and functionally distinct actin filaments". Journal of Cell Science 128 (11): 2009–19. June 2015. doi:10.1242/jcs.165563. PMID 25788699. 
  5. ↑ "Stresses at the cell surface during animal cell morphogenesis". Current Biology 24 (10): R484-94. May 2014. doi:10.1016/j.cub.2014.03.059. PMID 24845681. 
  6. ↑ "Organizing the cell cortex: the role of ERM proteins". Nature Reviews. Molecular Cell Biology 11 (4): 276–87. April 2010. doi:10.1038/nrm2866. PMID 20308985. 
  7. ↑ "Spectrin-based skeleton as an actor in cell signaling". Cellular and Molecular Life Sciences 69 (2): 191–201. January 2012. doi:10.1007/s00018-011-0804-5. PMID 21877118. 
  8. ↑ "Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork". Physical Review X 7 (1). 2017. doi:10.1103/PhysRevX.7.011031. PMID 28690919. Bibcode: 2017PhRvX...7a1031S. 
  9. ↑ "Active elastic network: cytoskeleton of the red blood cell". Physical Review E 75 (1 Pt 1): 011921. January 2007. doi:10.1103/PhysRevE.75.011921. PMID 17358198. Bibcode: 2007PhRvE..75a1921G. 
  10. ↑ "Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons". Science 339 (6118): 452–6. January 2013. doi:10.1126/science.1232251. PMID 23239625. Bibcode: 2013Sci...339..452X. 
  11. ↑ "The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure". Journal of Cell Science 131 (11): jcs215897. June 2018. doi:10.1242/jcs.215897. PMID 29739876. 
  12. ↑ 12.0 12.1 12.2 12.3 12.4 "The actin cortex at a glance". J Cell Sci 131 (14). July 2018. doi:10.1242/jcs.186254. PMID 30026344. 
  13. ↑ "Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding". Nature 469 (7329): 226–30. January 2011. doi:10.1038/nature09642. PMID 21196934. Bibcode: 2011Natur.469..226S. 
  14. ↑ "Cdk1-dependent mitotic enrichment of cortical myosin II promotes cell rounding against confinement". Nature Cell Biology 17 (2): 148–59. February 2015. doi:10.1038/ncb3098. PMID 25621953. 
  15. ↑ Lancaster, O (2013). "Mitotic Rounding Alters Cell Geometry to Ensure Efficient Bipolar Spindle Formation". Developmental Cell 25 (3): 270–283. doi:10.1016/j.devcel.2013.03.014. PMID 23623611. 
  16. ↑ Cattin, Cedric (2015). "Mechanical control of mitotic progression in single animal cells". PNAS 112 (36): 11258–11263. doi:10.1073/pnas.1502029112. PMID 26305930. Bibcode: 2015PNAS..11211258C. 
  17. ↑ Maddox, A (2003). "RhoA is required for cortical retraction and rigidity during mitotic cell rounding". J. Cell Biol. 160 (2): 255–265. doi:10.1083/jcb.200207130. PMID 12538643. PMC 2172639. https://rupress.org/jcb/article/160/2/255/32948/RhoA-is-required-for-cortical-retraction-and. 
  18. ↑ Fujibuchi, T (2005). "AIP1/WDR1 supports mitotic cell rounding". Biochem. Biophys. Res. Commun. 327 (1): 268–275. doi:10.1016/j.bbrc.2004.11.156. PMID 15629458. https://www.sciencedirect.com/science/article/abs/pii/S0006291X04027317. 
  19. ↑ Kunda, P (2008). "Moesin Controls Cortical Rigidity, Cell Rounding, and Spindle Morphogenesis during Mitosis". Current Biology 18 (2): 91–101. doi:10.1016/j.cub.2007.12.051. PMID 18207738. 
  20. ↑ Matthews, H (2013). "Changes in Ect2 Localization Couple Actomyosin-Dependent Cell Shape Changes to Mitotic Progression". Developmental Cell 23 (2): 371–383. doi:10.1016/j.devcel.2012.06.003. PMID 22898780. 
  21. ↑ Rosa, A (2015). "Ect2/Pbl acts via Rho and polarity proteins to direct the assembly of an isotropic actomyosin cortex upon mitotic entry". Developmental Cell 32 (5): 604–616. doi:10.1016/j.devcel.2015.01.012. PMID 25703349. 
  22. ↑ Toyoda, Y (2017). "Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding". Nature Communications 8 (1): 1266. doi:10.1038/s41467-017-01147-6. PMID 29097687. Bibcode: 2017NatCo...8.1266T. 
  23. ↑ "Cytokinesis in animal cells". Annual Review of Cell and Developmental Biology 28: 29–58. November 2012. doi:10.1146/annurev-cellbio-101011-155718. PMID 22804577. 
  24. ↑ "The actin cytoskeleton in cancer cell motility". Clinical & Experimental Metastasis 26 (4): 273–87. April 2009. doi:10.1007/s10585-008-9174-2. PMID 18498004. 

Further reading



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