Biology:FANCF

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Short description: Protein-coding gene in the species Homo sapiens


A representation of the 3D structure of the protein myoglobin showing turquoise α-helices.
Generic protein structure example

Fanconi anemia group F protein is a protein that in humans is encoded by the FANCF gene.[1][2]

Interactions

FANCF has been shown to interact with Fanconi anemia, complementation group C,[3][4] FANCG,[3][4][5][6] FANCA[3][4][7] and FANCE.[3][8]

Function

FANCF is an adaptor protein that plays a key role in the proper assembly of the FA core complex.[3] The FA core complex is composed of eight proteins (FANCA, -B, -C, -E, -F, -G, -L and -M).[9][10] FANCF stabilizes the interaction between the FANCC/FANCE subcomplex and the FANCA/FANCG subcomplex and locks the whole FA core complex in a conformation that is essential to perform its function in DNA repair.[3]

The FA core complex is a nuclear core complex that is essential for the monoubiquitination of FANCD2 and this modified form of FANCD2 colocalizes with BRCA1, RAD51 and PCNA in foci that also contain other DNA repair proteins.[3] All these proteins function together to facilitate DNA interstrand cross-link repair. They also function in other DNA damage response repair processes including recovering and stabilizing stalled replication forks.[10] FoxF1 protein also interacts with the FA protein core and induces its binding to chromatin to promote DNA repair.[10]

Cancer

DNA damage appears to be the primary underlying cause of cancer,[11] and deficiencies in expression of DNA repair genes appear to underlie many forms of cancer.[12][13] If DNA repair is deficient, DNA damage tends to accumulate. Such excess DNA damage may increase mutations due to error-prone translesion synthesis. Excess DNA damage may also increase epigenetic alterations due to errors during DNA repair.[14][15] Such mutations and epigenetic alterations may give rise to cancer.

Reductions in expression of DNA repair genes (usually caused by epigenetic alterations) are very common in cancers, and are most often much more frequent than mutational defects in DNA repair genes in cancers.[citation needed] (Also see Frequencies of epimutations in DNA repair genes.)

Methylation of the promoter region of the FANCF gene causes reduced expression of FANCF protein.[16]

The frequencies of FANCF promoter methylation in several different cancers is indicated in the table.

Frequency of FANCF promoter methylation in sporadic cancers
Cancer Frequency Ref.
Epithelian ovarian cancer 32% [17]
Cervical carcinoma 30% [18]
Ovarian cancer 21%-28% [16][19]
Head and neck squamous carcinomas 15% [20]
Non-small cell lung cancer 14% [20][21]
Male germ cell tumor 6% [22]

In invasive breast cancers, microRNA-210 (miR-210) was increased, along with decreased expression of FANCF, where FANCF was one of the likely targets of miR-210.[23]

Although mutations in FANCF are ordinarily not observed in human tumors, an FANCF-deficient mouse model was prone to ovarian cancers.[24]

FANCF appears to be one of about 26 DNA repair genes that are epigenetically repressed in various cancers (see Cancer epigenetics).

Infertility

The gonads of FANCF mutant mice function abnormally, having compromised follicle development and spermatogenesis as has been observed in other Fanconi anemia mouse models and in Fanconi anemia patients.[24] Histological examination of the testes from FANCF-deficient mice showed that the seminiferous tubules were devoid of germ cells. At 14 weeks of age, FANCF-deficient female mice were almost or completely devoid of primordial follicles. It was concluded that FANCF-deficient mice display a rapid depletion of primordial follicles at a young age resulting in advanced ovarian aging.[24]

References

  1. ↑ "Evidence for at least eight Fanconi anemia genes". American Journal of Human Genetics 61 (4): 940–4. October 1997. doi:10.1086/514881. PMID 9382107. 
  2. ↑ "Entrez Gene: FANCF Fanconi anemia, complementation group F". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=2188. 
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 "The Fanconi anemia gene product FANCF is a flexible adaptor protein". The Journal of Biological Chemistry 279 (38): 39421–30. September 2004. doi:10.1074/jbc.M407034200. PMID 15262960. 
  4. ↑ 4.0 4.1 4.2 "The Fanconi anemia protein FANCF forms a nuclear complex with FANCA, FANCC and FANCG". Human Molecular Genetics 9 (18): 2665–74. November 2000. doi:10.1093/hmg/9.18.2665. PMID 11063725. 
  5. ↑ "Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid systems". Blood 102 (1): 136–41. July 2003. doi:10.1182/blood-2002-11-3517. PMID 12649160. 
  6. ↑ "Direct interactions of the five known Fanconi anaemia proteins suggest a common functional pathway". Human Molecular Genetics 10 (4): 423–9. February 2001. doi:10.1093/hmg/10.4.423. PMID 11157805. 
  7. ↑ "A novel ubiquitin ligase is deficient in Fanconi anemia". Nature Genetics 35 (2): 165–70. October 2003. doi:10.1038/ng1241. PMID 12973351. 
  8. ↑ "FANCE: the link between Fanconi anaemia complex assembly and activity". The EMBO Journal 21 (13): 3414–23. July 2002. doi:10.1093/emboj/cdf355. PMID 12093742. 
  9. ↑ "Fanconi anaemia and the repair of Watson and Crick DNA crosslinks". Nature 493 (7432): 356–63. January 2013. doi:10.1038/nature11863. PMID 23325218. Bibcode: 2013Natur.493..356K. 
  10. ↑ 10.0 10.1 10.2 "Forkhead transcription factor FoxF1 interacts with Fanconi anemia protein complexes to promote DNA damage response". Oncotarget 7 (2): 1912–26. January 2016. doi:10.18632/oncotarget.6422. PMID 26625197. 
  11. ↑ "DNA damage responses: mechanisms and roles in human disease: 2007 G.H.A. Clowes Memorial Award Lecture". Molecular Cancer Research 6 (4): 517–24. April 2008. doi:10.1158/1541-7786.MCR-08-0020. PMID 18403632. 
  12. ↑ "The DNA damage response: ten years after". Molecular Cell 28 (5): 739–45. December 2007. doi:10.1016/j.molcel.2007.11.015. PMID 18082599. 
  13. ↑ "Molecular pathways: exploiting tumor-specific molecular defects in DNA repair pathways for precision cancer therapy". Clinical Cancer Research 20 (23): 5882–7. December 2014. doi:10.1158/1078-0432.CCR-14-1165. PMID 25451105. 
  14. ↑ "Double strand breaks can initiate gene silencing and SIRT1-dependent onset of DNA methylation in an exogenous promoter CpG island". PLOS Genetics 4 (8): e1000155. 2008. doi:10.1371/journal.pgen.1000155. PMID 18704159. 
  15. ↑ "DNA damage, homology-directed repair, and DNA methylation". PLOS Genetics 3 (7): e110. July 2007. doi:10.1371/journal.pgen.0030110. PMID 17616978. 
  16. ↑ 16.0 16.1 "Disruption of the Fanconi anemia-BRCA pathway in cisplatin-sensitive ovarian tumors". Nature Medicine 9 (5): 568–74. May 2003. doi:10.1038/nm852. PMID 12692539. 
  17. ↑ "Promoter Hypermethylation of FANCF and Susceptibility and Prognosis of Epithelial Ovarian Cancer". Reproductive Sciences 23 (1): 24–30. January 2016. doi:10.1177/1933719115612136. PMID 26507869. 
  18. ↑ "Promoter hypermethylation of FANCF: disruption of Fanconi Anemia-BRCA pathway in cervical cancer". Cancer Research 64 (9): 2994–7. May 2004. doi:10.1158/0008-5472.can-04-0245. PMID 15126331. 
  19. ↑ "Promoter hypermethylation of FANCF plays an important role in the occurrence of ovarian cancer through disrupting Fanconi anemia-BRCA pathway". Cancer Biology & Therapy 5 (3): 256–60. March 2006. doi:10.4161/cbt.5.3.2380. PMID 16418574. 
  20. ↑ 20.0 20.1 "Inactivation of the Fanconi anemia/BRCA pathway in lung and oral cancers: implications for treatment and survival". Oncogene 23 (4): 1000–4. January 2004. doi:10.1038/sj.onc.1207256. PMID 14647419. 
  21. ↑ "CHFR methylation strongly correlates with methylation of DNA damage repair and apoptotic pathway genes in non-small cell lung cancer". Discovery Medicine 19 (104): 151–8. March 2015. PMID 25828518. 
  22. ↑ "Role of promoter hypermethylation in Cisplatin treatment response of male germ cell tumors". Molecular Cancer 3: 16. May 2004. doi:10.1186/1476-4598-3-16. PMID 15149548. 
  23. ↑ "Breast cancer signatures for invasiveness and prognosis defined by deep sequencing of microRNA". Proceedings of the National Academy of Sciences of the United States of America 109 (8): 3024–9. February 2012. doi:10.1073/pnas.1200010109. PMID 22315424. Bibcode: 2012PNAS..109.3024V. 
  24. ↑ 24.0 24.1 24.2 "Fancf-deficient mice are prone to develop ovarian tumours". The Journal of Pathology 226 (1): 28–39. January 2012. doi:10.1002/path.2992. PMID 21915857. 

Further reading

External links



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