Biology:KIAA0232
KIAA0232 is a nuclear phosphoserine protein which in humans is encoded by the KIAA0232 gene.
Gene
KIAA0232 is located at 4p16.1 neighboring TBC1 domain family member 14 and an uncharacterized locus. It has 10 exons which comprise its 4 known transcript variants.[1]
Expression
KIAA0232 is expressed fairly ubiquitously, but particularly highly in the brain relative to other tissues according to GEO normal tissue expression profiling.[2] Other notable areas of high expression identified by EST profiling include nerves, umbilical cord, and parathyroid.[3]
Homology
Paralogs
There are no known paralogs of KIAA0232.
Orthologs
KIAA0232 is conserved in most animals, including mammals, reptiles, birds, amphibians, insects, and as far back as Trichnella spiralis, a species of nematode. It is not found in fungi, plants, or prokaryotes.[4]
Protein
The KIAA0232 protein is 1395 amino acids in length with a molecular weight of 154.8kDa. It has higher than average frequencies of serine and glutamic acid residues as well as several multi-serine runs that are evolutionarily conserved. It has an isoelectric point of 4.52.[5][6]
Domains
KIAA0232 is largely composed of DUF4603.[7]
Subcellular localization
KIAA0232 is predicted to have nuclear localization.[5]
Post-translational modification
KIAA0232 is predicted by the tools at ExPASy to be highly phosphorylated, with 15 tyrosine specific sites, 25 threonine specific sites, and 103 serine specific sites.[8]
Interacting proteins
KIAA0232 is experimentally known to interact with YWHAZ,[9] DYRK1A, and DYRK1B.[10] via two hybrid interaction experiments. YWHAZ is an apoptotic path regulator known to bind to phosphoserine proteins. DYRK1A/B are dual-specificity tyrosine kinases. This corroborates the post-translational modification predictions.
Clinical significance
References
- ↑ "KIAA0232 KIAA0232 [Homo sapiens (human) - Gene - NCBI"]. https://www.ncbi.nlm.nih.gov/gene/9778.
- ↑ "NCBI GEO Expression Profiles". https://www.ncbi.nlm.nih.gov/geo/tools/profileGraph.cgi?ID=GDS424:61852_at.
- ↑ "EST Profile - Hs.79276". https://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.79276.
- ↑ "BLAST: Basic Local Alignment Search Tool". http://blast.ncbi.nlm.nih.gov/Blast.cgi.
- ↑ 5.0 5.1 "SAPS, Statistical Analysis of Protein Sequence". SDSC Biology Workbench. http://workbench.sdsc.edu/.
- ↑ "PI, Isoelectric point determination". SDSC Biology Workbench. http://seqtool.sdsc.edu/.
- ↑ "RCSB Protein Data Bank". http://www.rcsb.org/pdb/home/home.do.
- ↑ "NetPhos 2.0 Server". http://www.cbs.dtu.dk/services/NetPhos/.
- ↑ Bandyopadhyay, Sourav; Chiang, Chih-yuan; Srivastava, Jyoti; Gersten, Merril; White, Suhaila; Bell, Russell; Kurschner, Cornelia; Martin, Christopher H et al. (2010). "A human MAP kinase interactome". Nature Methods 7 (10): 801–805. doi:10.1038/nmeth.1506. PMID 20936779.
- ↑ Varjosalo, Markku; Keskitalo, Salla; Van Drogen, Audrey; Nurkkala, Helka; Vichalkovski, Anton; Aebersold, Ruedi; Gstaiger, Matthias (2013-04-25). "The Protein Interaction Landscape of the Human CMGC Kinase Group" (in en). Cell Reports 3 (4): 1306–1320. doi:10.1016/j.celrep.2013.03.027. ISSN 2211-1247. PMID 23602568.
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