Biology:NCK2

Short description: Protein-coding gene in the species Homo sapiens

Generic protein structure example

Cytoplasmic protein NCK2 (also known as NCK-beta and Grb4) is a protein that in humans is encoded by the NCK2 gene.

Function

NCK belongs to family of adaptor proteins. There are two mammalian NCK genes, NCK1 and NCK2. NCK1 is located in chromosome 3 and NCK2 is located in chromosome 2. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Interactions

NCK2 has been shown to interact with:

• Epidermal growth factor receptor,
• LIMS1,
• PDGFRB,
• PTK2,
• T-cell surface glycoprotein CD3 epsilon chain and
• TrkB.

Further reading

• "The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors". Molecular and Cellular Biology 12 (12): 5824–33. Dec 1992. doi:10.1128/MCB.12.12.5824. PMID 1333047. 
• "Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways". Molecular Biology of the Cell 9 (12): 3367–82. Dec 1998. doi:10.1091/mbc.9.12.3367. PMID 9843575. 
• "The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells". Molecular and Cellular Biology 19 (3): 2425–34. Mar 1999. doi:10.1128/mcb.19.3.2425. PMID 10022929. 
• "Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck". The Journal of Biological Chemistry 274 (9): 5542–9. Feb 1999. doi:10.1074/jbc.274.9.5542. PMID 10026169. 
• "Characterization of Ggrb4, an adapter protein interacting with Bcr-Abl". Blood 96 (2): 618–24. Jul 2000. doi:10.1182/blood.V96.2.618. PMID 10887126. 
• "Nckbeta adapter regulates actin polymerization in NIH 3T3 fibroblasts in response to platelet-derived growth factor bb". Molecular and Cellular Biology 20 (21): 7867–80. Nov 2000. doi:10.1128/MCB.20.21.7867-7880.2000. PMID 11027258. 
• "Identification and kinetic analysis of the interaction between Nck-2 and DOCK180". FEBS Letters 491 (3): 193–9. Mar 2001. doi:10.1016/S0014-5793(01)02195-0. PMID 11240126. 
• "Grb4/Nckbeta acts as a nuclear repressor of v-Abl-induced transcription from c-jun/c-fos promoter elements". The Journal of Biological Chemistry 276 (46): 43419–27. Nov 2001. doi:10.1074/jbc.M107030200. PMID 11514578. 
• "The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals". Nature 413 (6852): 174–9. Sep 2001. doi:10.1038/35093123. PMID 11557983. Bibcode: 2001Natur.413..174C. 
• "Nck-2 interacts with focal adhesion kinase and modulates cell motility". The International Journal of Biochemistry & Cell Biology 34 (7): 791–805. Jul 2002. doi:10.1016/S1357-2725(02)00002-X. PMID 11950595. 
• "Brain-derived neurotrophic factor promotes interaction of the Nck2 adaptor protein with the TrkB tyrosine kinase receptor". Biochemical and Biophysical Research Communications 294 (5): 1087–92. Jun 2002. doi:10.1016/S0006-291X(02)00606-X. PMID 12074588. 
• "Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation". Cell 109 (7): 901–12. Jun 2002. doi:10.1016/S0092-8674(02)00799-7. PMID 12110186. 
• "Solution structure and backbone dynamics of the functional cytoplasmic subdomain of human ephrin B2, a cell-surface ligand with bidirectional signaling properties". Biochemistry 41 (36): 10942–9. Sep 2002. doi:10.1021/bi025815u. PMID 12206665. 
• "The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptor". Experimental Cell Research 279 (1): 21–33. Sep 2002. doi:10.1006/excr.2002.5576. PMID 12213210. 
• "Interaction of Axl receptor tyrosine kinase with C1-TEN, a novel C1 domain-containing protein with homology to tensin". Biochemical and Biophysical Research Communications 299 (5): 793–800. Dec 2002. doi:10.1016/S0006-291X(02)02718-3. PMID 12470648. 
• "Structural and functional insights into PINCH LIM4 domain-mediated integrin signaling". Nature Structural Biology 10 (7): 558–64. Jul 2003. doi:10.1038/nsb938. PMID 12794636. 
• "90-kDa ribosomal S6 kinase is a direct target for the nuclear fibroblast growth factor receptor 1 (FGFR1): role in FGFR1 signaling". The Journal of Biological Chemistry 279 (28): 29325–35. Jul 2004. doi:10.1074/jbc.M311144200. PMID 15117958. 
• "Structural insight into the binding diversity between the Tyr-phosphorylated human ephrinBs and Nck2 SH2 domain". The Journal of Biological Chemistry 280 (19): 19205–12. May 2005. doi:10.1074/jbc.M500330200. PMID 15764601. 
• "Tyrosine phosphorylation of the well packed ephrinB cytoplasmic beta-hairpin for reverse signaling. Structural consequences and binding properties". The Journal of Biological Chemistry 278 (27): 24714–20. Jul 2003. doi:10.1074/jbc.M210625200. PMID 12606549. 
• "NCK2 is significantly associated with opiates addiction in African-origin men". TheScientificWorldJournal 2013: 748979. 2013. doi:10.1155/2013/748979. PMID 23533358. 

External links

• Nck2 Info with links in the Cell Migration Gateway