Biology:NDUFB1
 Generic protein structure example |
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 is an enzyme that in humans is encoded by the NDUFB1 gene.[1][2] NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 1, 7kDa is an accessory subunit of the NADH dehydrogenase (ubiquinone) complex, located in the mitochondrial inner membrane. It is also known as Complex I and is the largest of the five complexes of the electron transport chain.[3]
Structure
The NDUFB1 gene, located on the q arm of chromosome 14 in position 32.12, is 5,687 base pairs long. The NDUFB1 protein weighs 7 kDa and is composed of 58 amino acids.[4][5] NDUFB1 is a subunit of the enzyme NADH dehydrogenase (ubiquinone), the largest of the respiratory complexes. The structure is L-shaped with a long, hydrophobic transmembrane domain and a hydrophilic domain for the peripheral arm that includes all the known redox centers and the NADH binding site.[3] NDUFB1 is one of about 31 hydrophobic subunits that form the transmembrane region of Complex I. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of Complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and that the hydrophobic domain acts as an anchor for the NADH dehydrogenase (ubiquinone) complex at the inner mitochondrial membrane.[2]
Function
The human NDUFB1 gene codes for a subunit of Complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone.[2] However, NDUFB1 is an accessory subunit of the complex that is believed not to be involved in catalysis.[6] Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.[3]
References
- ↑ "Intron based radiation hybrid mapping of 15 complex I genes of the human electron transport chain". Cytogenetics and Cell Genetics 82 (1–2): 115–119. November 1998. doi:10.1159/000015082. PMID 9763677.
- ↑ 2.0 2.1 2.2 "Entrez Gene: NDUFB1 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 1, 7kDa". https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=4707.
- ↑ 3.0 3.1 3.2 "Chapter 18". Fundamentals of biochemistry: life at the molecular level (4th ed.). Hoboken, NJ: Wiley. 2013. pp. 581–620. ISBN 978-0-470-54784-7.
- ↑ "Integration of cardiac proteome biology and medicine by a specialized knowledgebase". Circulation Research 113 (9): 1043–1053. October 2013. doi:10.1161/CIRCRESAHA.113.301151. PMID 23965338.
- ↑ "NADH dehydrogenase [ubiquinone 1 beta subcomplex subunit 1"]. Cardiac Organellar Protein Atlas Knowledgebase (COPaKB). https://amino.heartproteome.org/web/protein/O75438.
- ↑ "NDUFB1 - NADH dehydrogenase [ubiquinone 1 beta subcomplex subunit 1"]. The UniProt Consortium. https://www.uniprot.org/uniprot/O75438.
Further reading
- "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–1178. October 2005. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R.
- "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Research 10 (10): 1546–1560. October 2000. doi:10.1101/gr.140200. PMID 11042152.
- "cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed". Biochemical and Biophysical Research Communications 253 (2): 415–422. December 1998. doi:10.1006/bbrc.1998.9786. PMID 9878551.
- "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning". Proceedings of the National Academy of Sciences of the United States of America 95 (14): 8175–8180. July 1998. doi:10.1073/pnas.95.14.8175. PMID 9653160. Bibcode: 1998PNAS...95.8175M.
- "Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits.". Biochemical and Biophysical Research Communications 241 (2): 589–594. 1998. doi:10.1006/bbrc.1997.7707. PMID 9425316.
This article incorporates text from the United States National Library of Medicine, which is in the public domain.
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