Biology:PRPF8
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Pre-mRNA-processing-splicing factor 8 is a protein that in humans is encoded by the PRPF8 gene.[1][2]
Function
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.[3]
Interactions
PRPF8 has been shown to interact with WDR57[4][5] and EFTUD2.[5]
References
- ↑ "Mutations in the pre-mRNA splicing factor gene PRPC8 in autosomal dominant retinitis pigmentosa (RP13)". Human Molecular Genetics 10 (15): 1555–62. Jul 2001. doi:10.1093/hmg/10.15.1555. PMID 11468273.
- ↑ "The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes". RNA 5 (7): 893–908. Jul 1999. doi:10.1017/S1355838299990520. PMID 10411133.
- ↑ "Entrez Gene: PRPF8 PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10594.
- ↑ "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. 2007. doi:10.1038/msb4100134. PMID 17353931.
- ↑ 5.0 5.1 "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein". Molecular and Cellular Biology 18 (11): 6756–66. Nov 1998. doi:10.1128/mcb.18.11.6756. PMID 9774689.
Further reading
- "Prp8 protein: at the heart of the spliceosome". RNA 11 (5): 533–57. May 2005. doi:10.1261/rna.2220705. PMID 15840809.
- "A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein". Proceedings of the National Academy of Sciences of the United States of America 87 (8): 3082–6. Apr 1990. doi:10.1073/pnas.87.8.3082. PMID 2139226. Bibcode: 1990PNAS...87.3082G.
- "The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome". Proceedings of the National Academy of Sciences of the United States of America 86 (22): 8742–6. Nov 1989. doi:10.1073/pnas.86.22.8742. PMID 2479028. Bibcode: 1989PNAS...86.8742P.
- "20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition". Proceedings of the National Academy of Sciences of the United States of America 86 (16): 6038–42. Aug 1989. doi:10.1073/pnas.86.16.6038. PMID 2527369. Bibcode: 1989PNAS...86.6038B.
- "Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein". Nature 342 (6251): 819–21. Dec 1989. doi:10.1038/342819a0. PMID 2532307. Bibcode: 1989Natur.342..819A.
- "Prp16p, Slu7p, and Prp8p interact with the 3' splice site in two distinct stages during the second catalytic step of pre-mRNA splicing". RNA 1 (6): 584–97. Aug 1995. PMID 7489518.
- "Fine localization of the locus for autosomal dominant retinitis pigmentosa on chromosome 17p". American Journal of Human Genetics 57 (4): 962–5. Oct 1995. PMID 7573060.
- "A new locus for autosomal dominant retinitis pigmentosa on the short arm of chromosome 17". Human Molecular Genetics 3 (6): 915–8. Jun 1994. doi:10.1093/hmg/3.6.915. PMID 7951236.
- "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. Jan 1994. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- "Map refinement of locus RP13 to human chromosome 17p13.3 in a second family with autosomal dominant retinitis pigmentosa". American Journal of Human Genetics 58 (2): 347–55. Feb 1996. PMID 8571961.
- "The canonical GU dinucleotide at the 5' splice site is recognized by p220 of the U5 snRNP within the spliceosome". RNA 2 (3): 213–25. Mar 1996. PMID 8608445.
- "Domain analysis of human U5 RNA. Cap trimethylation, protein binding, and spliceosome assembly". The Journal of Biological Chemistry 271 (31): 19001–7. Aug 1996. doi:10.1074/jbc.271.31.19001. PMID 8702566.
- "Evidence that U5 snRNP recognizes the 3' splice site for catalytic step II in mammals". The EMBO Journal 16 (15): 4746–59. Aug 1997. doi:10.1093/emboj/16.15.4746. PMID 9303319.
- "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. Oct 1997. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein". Molecular and Cellular Biology 18 (11): 6756–66. Nov 1998. doi:10.1128/mcb.18.11.6756. PMID 9774689.
- "The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5' splice site". RNA 5 (2): 167–79. Feb 1999. doi:10.1017/S1355838299981785. PMID 10024169.
- "The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions". Journal of Molecular Biology 298 (4): 567–75. May 2000. doi:10.1006/jmbi.2000.3685. PMID 10788320.
- "Pre-mRNA splicing alters mRNP composition: evidence for stable association of proteins at exon-exon junctions". Genes & Development 14 (9): 1098–108. May 2000. doi:10.1101/gad.14.9.1098. PMID 10809668.
- "Expression map of human chromosome region 17p13.3, spanning the RP13 dominant retinitis pigmentosa locus, the Miller-Dieker lissencephaly syndrome (MDLS) region, and a putative tumour suppressor locus". Cytogenetics and Cell Genetics 88 (3–4): 225–9. 2000. doi:10.1159/000015556. PMID 10828595.
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