Medicine:Pexastimogene devacirepvec

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JX-594 is an oncolytic virus is designed to target and destroy cancer cells.[1] It is also known as Pexa-Vec,[2] INN pexastimogene devacirepvec[3]) and was constructed in Dr. Edmund Lattime's lab at Thomas Jefferson University, tested in clinical trials on melanoma patients, and licensed and further developed by SillaJen. JX-594 is a modified Copenhagen[4] strain (or Wyeth strain[3]) vaccinia poxvirus engineered by addition of the GM-CSF gene and deletion of the thymidine kinase gene which limits viral replication to cells with high levels of thymidine kinase, typically seen in cancer cells with a mutated RAS or p53 gene.[5] The virus also has the LacZ gene insertion under control of the p7.5 promoter.[3] The virus kills the infected/cancer cells by lysis and also expresses GM-CSF which may help initiate an anti-tumour immune response.[6][7] [8]

It has orphan drug designation from US Food and Drug Administration and EUMA[clarification needed] for the treatment of hepatocellular carcinoma (liver cancer).[2]

In clinical trials doses have been administered by intratumoral or intravenous injection.[3]

Technology

Pexa-Vec (JX-594) is the most advanced product candidate from SillaJen's proprietary SOLVE™ (Selective Oncolytic Vaccinia Engineering) platform.[9][non-primary source needed] SOLVE[9] is used to optimize virus targeting to specific cancer types, to select transgenes to include into the viral genome, and to optimize viral infection and/or replication selectively through targeted mutations.

Oncolytic viruses could have 3-prolonged attack on cancer: direct cell lysis with replication and spread, immune activation, and antivascular[10][11][12][13]

  • Tumor selective intratumoral replication of the virus leads to lysis of the infected cancer cell and spread to adjacent cancer cells[14][non-primary source needed]
  • Induction of tumor-specific cytotoxic T-lymphocytes and “arming” for expression of therapeutic transgene products (e.g. GM-CSF) enhance immune response to the tumor[14]
  • Blood flow to tumors can be blocked following intratumoral replication and spread[14]JX-900 (VVDD):[15][non-primary source needed] VVDD Platform: Next-gen enhanced oncolytic immunotherapy.[16]

JX-900 (VVDD):[15] VVDD Platform: Next-gen enhanced oncolytic immunotherapy.[16]

  • JX-900 is a series of modified vaccinia vaccine (Western Reserve strain) with enhanced oncolytic potency
  • Attenuation via “Double-Deletion” : thymidine kinase and vaccinia growth factor gene inactivation
  • JX-929 (vvDD expressing CD for 5-FU pro-drug) experience in solid tumors (CRC and melanoma)
  • JX-970 (vvDD expressing [[granulocyte-macrophage colony-stimulating factor]) next generation of the VVDD series

Clinical trials

A phase 3 randomized, open-label, clinical trial of Pexa-Vec plus sorafenib versus sorafenib is being conducted on patients with advanced hepatocellular carcinoma who have not previously received any systemic therapy. The study is being done to determine and compare overall survival for patients in the two treatment arms.[17][non-primary source needed] The study is Sponsored by SillaJen, Inc.[18]

Mechanism of Action of Pexa-Vec (Jx-594)

The experimental therapy, Pexa-Vec, is an attenuated vaccinia virus engineered to stimulate anti-tumor immunity and directly lyse tumor cells. Pexa-Vec has cancer selectivity through the deactivation of its thymidine kinase gene, and it has been engineered to express the granulocyte-macrophage colony-stimulating factor gene to stimulate a systemic anti-tumor immune response. Researchers believe that Pexa-Vec may be a systemic treatment of hepatocellular carcinoma by inducing tumor necrosis and shrinkage of both injected and non-injected tumors after direct intratumoral delivery. Final data from a randomized dose-ranging phase 2 study of Pexa-Vec[19] in mainly sorafenib naïve patients with advanced hepatocellular carcinoma demonstrated that the risk of death for patients who received Pexa-Vec at the high dose was markedly reduced (by nearly 60 percent; hazard ratio = 0.41) when compared to patients randomized to a low dose control (one-tenth of the high dose). The median overall survival for high and low dose groups was 14.1 months versus 6.7 months, respectively (p = 0.020 for superiority of the high dose). Pexa-Vec was well tolerated, with patients experiencing transient flu-like symptoms that generally resolved within 24 hours.[17][1]


Clinical trials investigating Pexa-Vec ((As of June 2018))
Indication Phase Status Notes Sponsor Ref
Hepatocellular carcinoma III Recruiting Combined with sorafenib SillaJen NCT02562755
Solid Tumors II Recruiting Combined with metronomic cyclophosphamide Investigator NCT02630368
Renal Cell Carcinoma 2L I Recruiting Combined with REGN2810 SillaJen NCT03294083
Colorectal Cancer 2L/3L I Recruiting Combined with PD-L1 and CTLA4 Investigator
Liver Cancer I Recruiting Combined with Nivolumab Transgene NCT03071094
Solid Tumors I Recruiting Combined with Ipilimumab Investigator NCT02977156

Study Design

Participants will be randomly assigned to one of two treatment arms, having an equal chance of receiving either Pexa-Vec followed by sorafenib, or sorafenib alone.[citation needed]

Arm A: Pexa-Vec followed by sorafenib

  • Participants will visit the study center approximately 14 times over 18 weeks.[17]
  • All Pexa-Vec treatments (3) will be given by intratumoral injections into liver tumors.
  • Following Pexa-Vec injection series completion, patients will receive sorafenib starting at week 6 of the study

Arm B: sorafenib

  • Participants will visit the study center approximately 12 times over 18 weeks and receive sorafenib as per standard of care.[17]

Pipeline candidates

JX-Next Generation

Novel oncolytic viruses in SillaJen pipeline are engineered through the Selective Oncolytic Vaccinia Engineering (SOLVE) platform.[20]

JX-929

JX-929 is derived from Western Reserve strain vaccinia virus. JX-929's tumor selectivity has been optimized through deletion of thymidine kinase and vaccinia growth factor. JX-929 has been administered as a monotherapy to patients with breast, colorectal, and pancreatic cancer via intratumoral & intravenous injections in a Phase 1, dose escalation clinical trial. This Phase 1 study showed delivery to and replication within tumors [21] both IT[22] and IV.[23][clarification needed]

JX-970

JX-970 is also derived from a Western Reserve strain vaccinia virus and utilizes the same tumor selectivity mechanisms as JX-929. In addition, it expresses granulocyte-macrophage colony-stimulating factor to stimulate immune responses. In nonclinical studies, the JX-970 backbone exerted a tumor debulking effect and at the same time demonstrated a selective preference for tumor tissues.[22] The precursor of JX-970 is JX-963 which demonstrated efficacy in pre-clinical studies.[citation needed]

References

  1. ↑ Un virus contre le cancer 25 March 2012, Radio Canada (in French)
  2. ↑ 2.0 2.1 Jennerex Granted FDA Orphan Drug Designation for Pexa-Vec in Hepatocellular Carcinoma (HCC)
  3. ↑ 3.0 3.1 3.2 3.3 Phase 1 Study of Intratumoral Pexa-Vec (JX-594), an Oncolytic and Immunotherapeutic Vaccinia Virus, in Pediatric Cancer Patients
  4. ↑ Transgene Presents Data on Improved Cytotoxic Activity of Oncolytic Viruses Expressing Intrabodies in Resistant Tumor Cell Lines. October 2016
  5. ↑ Bos, JL (September 1, 1989). "ras oncogenes in human cancer: a review.". Cancer Research 49 (17): 4682–9. PMID 2547513. http://cancerres.aacrjournals.org/content/49/17/4682.long. 
  6. ↑ "NCI Drug Dictionary". National Cancer Institute. 2011-02-02. http://www.cancer.gov/drugdictionary?CdrID=579292. 
  7. ↑ "Novel Cancer-Targeting Virus Therapy Shows Efficacy in Early-Stage Trial". 31 August 2011. http://www.cancernetwork.com/cancer-genetics/content/article/10165/1939688. 
  8. ↑ Breitbach at al. (2011). "Intravenous delivery of a multi-mechanistic cancer-targeted oncolytic poxvirus in humans". Nature 477 (7362): 99–102. doi:10.1038/nature10358. PMID 21886163. Bibcode: 2011Natur.477...99B. 
  9. ↑ 9.0 9.1 "SOLVE > Technology > Sillajen" (in ko). http://www.sillajen.com/eng/sub/eng_sub.aspx?s_code=0301000000. 
  10. ↑ Breitbach, Caroline J.; Burke, James; Jonker, Derek; Stephenson, Joe; Haas, Andrew R.; Chow, Laura Q. M.; Nieva, Jorge; Hwang, Tae-Ho et al. (2011-08-31). "Intravenous delivery of a multi-mechanistic cancer-targeted oncolytic poxvirus in humans". Nature 477 (7362): 99–102. doi:10.1038/nature10358. ISSN 1476-4687. PMID 21886163. Bibcode: 2011Natur.477...99B. 
  11. ↑ Kirn, David H.; Thorne, Steve H. (January 2009). "Targeted and armed oncolytic poxviruses: a novel multi-mechanistic therapeutic class for cancer". Nature Reviews. Cancer 9 (1): 64–71. doi:10.1038/nrc2545. ISSN 1474-1768. PMID 19104515. 
  12. ↑ Breitbach, Caroline J.; Arulanandam, Rozanne; De Silva, Naomi; Thorne, Steve H.; Patt, Richard; Daneshmand, Manijeh; Moon, Anne; Ilkow, Carolina et al. (2013-02-15). "Oncolytic vaccinia virus disrupts tumor-associated vasculature in humans". Cancer Research 73 (4): 1265–1275. doi:10.1158/0008-5472.CAN-12-2687. ISSN 1538-7445. PMID 23393196. 
  13. ↑ Parker, Charles Thomas; Garrity, George M (2003-01-01). Parker, Charles Thomas; Garrity, George M. eds. Exemplar Abstract for Mycobacterium yongonense Kim et al. 2013 and Mycobacterium intracellulare yongonense (Kim et al. 2013) Castejon et al. 2018.. doi:10.1601/ex.23701. 
  14. ↑ 14.0 14.1 14.2 "SOLVE > Technology > Sillajen" (in ko). http://www.sillajen.com/eng/sub/eng_sub.aspx?s_code=0301000000. 
  15. ↑ 15.0 15.1 "JX-900 Series > Pipeline > Sillajen" (in ko). http://www.sillajen.com/eng/sub/eng_sub.aspx?s_code=0202000000. 
  16. ↑ 16.0 16.1 Zeh, Herbert J; Downs-Canner, Stephanie; McCart, J Andrea; Guo, Zong Sheng; Rao, Uma N M; Ramalingam, Lekshmi; Thorne, Stephen H; Jones, Heather L et al. (January 2015). "First-in-man Study of Western Reserve Strain Oncolytic Vaccinia Virus: Safety, Systemic Spread, and Antitumor Activity". Molecular Therapy 23 (1): 202–214. doi:10.1038/mt.2014.194. ISSN 1525-0016. PMID 25292189. 
  17. ↑ 17.0 17.1 17.2 17.3 "General Information | PHOCUS" (in en). http://www.pexavectrials.com/en/about-phocus/. 
  18. ↑ "Hepatocellular Carcinoma Study Comparing Vaccinia Virus Based Immunotherapy Plus Sorafenib vs Sorafenib Alone" (in en). https://clinicaltrials.gov/ct2/show/NCT02562755?term=JX-594&phase=2&rank=1. 
  19. ↑ Heo, Jeong; Breitbach, Caroline; Cho, Mong; Hwang, Tae-Ho; Kim, Chang Won; Jeon, Ung Bae; Woo, Hyun Young; Yoon, Ki Tae et al. (2013-05-20). "Phase II trial of Pexa-Vec (pexastimogene devacirepvec; JX-594), an oncolytic and immunotherapeutic vaccinia virus, followed by sorafenib in patients with advanced hepatocellular carcinoma" (in en). Journal of Clinical Oncology 31 (15_suppl): 4122. doi:10.1200/jco.2013.31.15_suppl.4122. 
  20. ↑ "JX-900 Series > Pipeline > Sillajen" (in ko). http://www.sillajen.com/eng/sub/eng_sub.aspx?s_code=0202000000. 
  21. ↑ Thorne, R. M.; Horne, R. B. (December 2007). "Comment onKhazanov et al.[2002] andKhazanov et al.[2006]". Journal of Geophysical Research: Space Physics 112 (A12): n/a. doi:10.1029/2007ja012268. ISSN 0148-0227. Bibcode: 2007JGRA..11212214T. 
  22. ↑ 22.0 22.1 Zeh, Herbert J.; Downs-Canner, Stephanie; McCart, J. Andrea; Guo, Zong Sheng; Rao, Uma N. M.; Ramalingam, Lekshmi; Thorne, Stephen H.; Jones, Heather L. et al. (January 2015). "First-in-man study of western reserve strain oncolytic vaccinia virus: safety, systemic spread, and antitumor activity". Molecular Therapy 23 (1): 202–214. doi:10.1038/mt.2014.194. ISSN 1525-0024. PMID 25292189. 
  23. ↑ Downs-Canner, Stephanie; Guo, Zong Sheng; Ravindranathan, Roshni; Breitbach, Caroline J; O'Malley, Mark E; Jones, Heather L; Moon, Anne; McCart, Judith Andrea et al. (August 2016). "Phase 1 Study of Intravenous Oncolytic Poxvirus (vvDD) in Patients With Advanced Solid Cancers". Molecular Therapy 24 (8): 1492–1501. doi:10.1038/mt.2016.101. ISSN 1525-0016. PMID 27203445. 



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