Biology:Dispase
Dispase is a protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I. It is found in some bacteria and can be isolated from culture filtrates of Bacillus polymyxa. It can be extracted, purified, and used in research. It can be particularly useful to separate embryonic epithelia and mesenchyme. Dispase II is specific for the cleavage of leucine-phenylalanine bonds. Dispase is often used to digest adhering primary cells in culture, since this treatment turned out to be milder than trypsin digestion (Sinclair et al., 2013).
A recent article also finds that dispase can digest serine-phenylalanine.[1]
Dispase intravitreal injection can be used in the modeling of proliferative vitreoretinopathy in different animals.[2][3][4]
References
- ↑ Weimer (2006). "A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases". Analytical Biochemistry 352 (1): 110–119. doi:10.1016/j.ab.2006.02.029. PMID 16564490.
- ↑ "THE CHARACTERISTICS OF RETINA AT THE DEVELOPMENT OF PROLIFERATIVE VITREORETINOPATHY IN RATS AFTER INTRAOCULAR INJECTION OF CONCANAVALIN A AND DISPASE". Ross Fiziol Zh Im I M Sechenova 101 (5): 572–85. May 2015. PMID 26263683.
- ↑ "A mouse model of proliferative vitreoretinopathy induced by dispase". Exp Eye Res 75 (5): 491–504. November 2002. PMID 12457862.
- ↑ Tikhonovich, Marina V.; Erdiakov, Aleksei K.; Gavrilova, Svetlana A. (2017-06-21). "Nonsteroid anti-inflammatory therapy suppresses the development of proliferative vitreoretinopathy more effectively than a steroid one" (in en). International Ophthalmology 38 (4): 1365–1378. doi:10.1007/s10792-017-0594-3. ISSN 0165-5701. PMID 28639085.
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