Biology:dnaC
| DNA replication gene dnaC | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | dnaC | ||||||
| Entrez | 948864 | ||||||
| RefSeq (Prot) | NP_418781.1 | ||||||
| UniProt | P0AEF0 | ||||||
| Other data | |||||||
| EC number | 2.7.7.7 | ||||||
| Chromosome | genome: 4.6 - 4.6 Mb | ||||||
| |||||||
dnaC is a loading factor that complexes with the C-terminus of helicase dnaB and inhibits it from unwinding the dsDNA at a replication fork.[1] A dnaB and dnaC associate near the dnaA bound origin for each of the ssDNA.[1] One dnaB-dnaC complex is oriented in the opposite direction to the other dnaB-dnaC complex due to the antiparallel nature of DNA. Because they are oriented in opposite directions, one dnaB-dnaC complex will complex with dnaA from the N-terminus of dnaB whereas the other dnaB-dnaC complex will complex with dnaA from the dnaC. After the assembly of dnaG onto the N-terminus of dnaB, dnaC is released and dnaB will be allowed to begin unwinding dsDNA to make room for DNA polymerase III to begin synthesizing the daughter strands.[1]
This interaction of dnaC with dnaB requires the hydrolysis of ATP.[2]
References
- ↑ 1.0 1.1 1.2 Slonczewski, Joan L.; Foster, John W.; Gillen, Kathy M. (2009). Microbiology: an evolving science (1st ed.). New York: W.W. Norton & Co.. ISBN 978-0-393-97857-5.
- ↑ "The Escherichia coli dnaC gene product. III. Properties of the dnaB-dnaC protein complex". The Journal of Biological Chemistry 257 (22): 13770–5. November 1982. PMID 6292205. http://www.jbc.org/cgi/content/abstract/257/22/13770.
External links
- DnaC+protein,+E+coli at the US National Library of Medicine Medical Subject Headings (MeSH)
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