Biology:Homing endonuclease

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Short description: Type of enzyme
Crystal structure of I-CreI bound to its DNA recognition sequence. The enzyme binds as a homodimer; one subunit is depicted in yellow, the other in pink. The enzyme is shown in surface representation; DNA molecule is shown as a collection of spheres, each colored according to its chemical element.

The homing endonucleases are a collection of endonucleases encoded either as freestanding genes within introns, as fusions with host proteins, or as self-splicing inteins. They catalyze the hydrolysis of genomic DNA within the cells that synthesize them, but do so at very few, or even singular, locations. Repair of the hydrolyzed DNA by the host cell frequently results in the gene encoding the homing endonuclease having been copied into the cleavage site, hence the term 'homing' to describe the movement of these genes. Homing endonucleases can thereby transmit their genes horizontally within a host population, increasing their allele frequency at greater than Mendelian rates.

Origin and mechanism

Although the origin and function of homing endonucleases is still being researched, the most established hypothesis considers them as selfish genetic elements,[1] similar to transposons, because they facilitate the perpetuation of the genetic elements that encode them independent of providing a functional attribute to the host organism.

Homing endonuclease recognition sequences are long enough to occur randomly only with a very low probability (approximately once every 7×109 bp),[2] and are normally found in one or very few instances per genome. Generally, owing to the homing mechanism, the gene encoding the endonuclease (the HEG, "homing endonuclease gene") is located within the recognition sequence which the enzyme cuts, thus interrupting the homing endonuclease recognition sequence and limiting DNA cutting only to sites that do not (yet) carry the HEG.

Prior to transmission, one allele carries the gene (HEG+) while the other does not (HEG−), and is therefore susceptible to being cut by the enzyme. Once the enzyme is synthesized, it breaks the chromosome in the HEG− allele, initiating a response from the cellular DNA repair system. The damage is repaired using recombination, taking the pattern of the opposite, undamaged DNA allele, HEG+, that contains the gene for the endonuclease. Thus, the gene is copied to the allele that initially did not have it and it is propagated through successive generations.[3] This process is called "homing".[3]

Nomenclature

Homing endonucleases are always indicated with a prefix that identifies their genomic origin, followed by a hyphen: "I-" for homing endonucleases encoded within an intron, "PI-" (for "protein insert") for those encoded within an intein. Some authors have proposed using the prefix "F-" ("freestanding") for viral enzymes and other natural enzymes not encoded by introns nor inteins,[4] and "H-" ("hybrid") for enzymes synthesized in a laboratory.[5] Next, a three-letter name is derived from the binominal name of the organism, taking one uppercase letter from the genus name and two lowercase letters from the specific name. (Some mixing is usually done for hybrid enzymes.) Finally, a Roman numeral distinguishes different enzymes found in the same organism:

Comparison to restriction enzymes

Homing endonucleases differ from Type II restriction enzymes in the several respects:[4]

  • Whereas Type II restriction enzymes bind short, usually symmetric, recognition sequences of 4 to 8 bp, homing endonucleases bind very long and in many cases asymmetric recognition sequences spanning 12 to 40 bp.
  • Homing endonucleases are generally more tolerant of substitutions in the recognition sequence. Minor variations in the recognition sequence usually decrease the activity of homing endonucleases, but often do not completely abolish it as often occurs with restriction enzymes.[10][11]
  • Homing endonucleases share structural motifs that suggest there are four families, whereas it has not been possible to determine simply recognisable and distinguishable families of Type II restriction enzymes.
  • Homing endonucleases act as monomers or homodimers, and often require associated proteins to regulate their activity[12] or form ribonucleoprotein complexes, wherein RNA is an integral component of the catalytic apparatus.[13] Type II restriction enzymes can also function alone, as monomers or homodimers,[14] or with additional protein subunits,[15] but the accessory subunits differ from those of the homing endonucleases. Thus, they can require restriction, modification, and specificity subunits for their action.[15]
  • Finally, homing endonucleases have a broader phylogenetic distribution, occurring in all three biological domains—the archaea, bacteria and eukarya. Type II restriction enzymes occur only in archaea, bacteria and certain viruses.[16][17][18] Homing endonucleases are also expressed in all three compartments of the eukaryotic cell: nuclei, mitochondria and chloroplasts. Open reading frames encoding homing endonucleases have been found in introns, inteins, and in freestanding form between genes, whereas genes encoding Type II restriction enzyme genes have been found only in freestanding form, almost always in close association with genes encoding cognate DNA modifying enzymes.[19] Thus, while the Type II restriction enzymes and homing endonucleases share the function of cleaving double-stranded DNA, they appear to have evolved independently.

Structural families

A
I-CreI dimer 1.png
 B
I-CreI dimer DNA.png


C
I-CreI dimer DNA 2.png
Dimer of the I-CreI homing endonuclease.[9] Alpha helices are shown in green and beta sheets in blue. A: The two small pink spheres in the center of the structure are two metal cations necessary for catalysis. The structure shows the saddle that beta strands create to accommodate the DNA. These strands contain the LAGLIDADG motifs that interact with the DNA minor groove. B & C: DNA atoms are shown as spheres, colored according to chemical element.
LAGLIDADG endonuclease
Identifiers
SymbolLAGLIDADG_1
PfamPF00961
Pfam clanCL0324
InterProIPR001982
CATH1af5
SCOP21af5 / SCOPe / SUPFAM
See clan entry for related Pfam families.
GIY-YIG endonuclease, catalytic
Identifiers
SymbolGIY-YIG
PfamPF01541
InterProIPR000305
PROSITEPS50164
CATH1mk0
SCOP21mk0 / SCOPe / SUPFAM

Currently there are six known structural families. Their conserved structural motifs are:[4]

  • LAGLIDADG: Every polypeptide has 1 or 2 LAGLIDADG motifs. The sequence LAGLIDADG is a conserved sequence of amino acids where each letter is a code that identifies a specific residue. This sequence is directly involved in the DNA cutting process. Those enzymes that have only one motif work as homodimers, creating a saddle that interacts with the major groove of each DNA half-site. The LAGLIDADG motifs contribute amino acid residues to both the protein-protein interface between protein domains or subunits, and to the enzyme's active sites. Enzymes that possess two motifs in a single protein chain act as monomers, creating the saddle in a similar way. The first structures to be determined of homing endonucleases (of PI-SceI and I-CreI, both reported in 1997) were both from the LAGLIDADG structural family.,[20][21] The following year, the first structure of a homing endonuclease (I-CreI) bound to its DNA target site was also reported.[9]
  • GIY-YIG: These have only one GIY-YIG motif, in the N-terminal region, that interacts with the DNA in the cutting site. The prototypic enzyme of this family is I-TevI which acts as a monomer. Separate structural studies have been reported of the DNA-binding and catalytic domains of I-TevI, the former bound to its DNA target and the latter in the absence of DNA.[22][23]
  • His-Cys box (Pfam PF05551): These enzymes possess a region of 30 amino acids that includes 5 conserved residues: two histidines and three cysteins. They co-ordinate the metal cation needed for catalysis. I-PpoI is the best characterized enzyme of this family and acts as a homodimer. Its structure was reported in 1998.[24] It is possibly related to the H-N-H family, as they share common features.[25]
  • H-N-H: (Pfam CL0263): These have a consensus sequence of approximately 30 amino acids. It includes two pairs of conserved histidines and one asparagine that create a zinc finger domain. I-HmuI (P34081) is the best characterized enzyme of this family, and acts as a monomer. Its structure was reported in 2004 (PDB: 1U3E​).[26]
  • PD-(D/E)xK (Pfam CL0236): These enzymes contain a canonical nuclease catalytic domain typically found in type II restriction endonucleases. The best characterized enzyme in this family, I-Ssp6803I (Q57253), acts as a tetramer. Its structure was reported in 2007 (PDB: 2OST​).[27] The overall fold is conserved in many endonuclease families, all of which belong to the PD-(D/E)xK superfamily.[28]
  • Vsr-like/EDxHD (DUF559, InterPro: IPR007569): These enzymes were discovered in the Global Ocean Sampling Metagenomic Database and first described in 2009. The term 'Vsr-like' refers to the presence of a C-terminal nuclease domain that displays recognizable homology to bacterial Very short patch repair (Vsr) endonucleases.[29] The structure has been solved in 2011, confirming the Vsr homology.[30] Is considered part of the PD-(D/E)xk superfamily.[28]

Domain architecture

Hom_end-associated Hint
PDB 1ef0 EBI.jpg
crystal structure of pi-scei miniprecursor
Identifiers
SymbolHom_end_hint
PfamPF05203
Pfam clanCL0363
InterProIPR007868
SCOP21gpp / SCOPe / SUPFAM
Intein motif of the larger LAGLIDADG Hom_end domain.

The yeast homing endonuclease PI-Sce is a LAGLIDADG-type endonuclease encoded as an intein that splices itself out of another protein (P17255). The high-resolution structure reveals two domains: an endonucleolytic centre resembling the C-terminal domain of Hedgehog proteins, and a Hint domain (Hedgehog/Intein) containing the protein-splicing active site.[31]

See also

References

  1. ↑ Edgell DR (February 2009). "Selfish DNA: homing endonucleases find a home". Curr Biol 19 (3): R115–R117. doi:10.1016/j.cub.2008.12.019. PMID 19211047. 
  2. ↑ Jasin M (Jun 1996). "Genetic manipulation of genomonth with rare-cutting endonucleases". Trends Genet 12 (6): 224–8. doi:10.1016/0168-9525(96)10019-6. PMID 8928227. 
  3. ↑ 3.0 3.1 "Homing endonuclease genes: the rise and fall and rise again of a selfish element". Curr Opin Genet Dev 14 (6): 609–15. December 2004. doi:10.1016/j.gde.2004.09.010. PMID 15531154. 
  4. ↑ 4.0 4.1 4.2 "Homing endonucleases: keeping the house in order". Nucleic Acids Res 25 (17): 3379–88. September 1995. doi:10.1093/nar/25.17.3379. PMID 9254693. 
  5. ↑ 5.0 5.1 "Design, activity, and structure of a highly specific artificial endonuclease". Mol. Cell 10 (4): 895–905. October 2002. doi:10.1016/S1097-2765(02)00690-1. PMID 12419232. 
  6. ↑ "Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae". J Biol Chem 265 (12): 6726–33. April 1990. doi:10.1016/S0021-9258(19)39210-5. PMID 2139027. 
  7. ↑ "Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase". Science 250 (4981): 651–7. November 1990. doi:10.1126/science.2146742. PMID 2146742. Bibcode: 1990Sci...250..651K. 
  8. ↑ "Intervening sequences in an Archaea DNA polymerase gene". PNAS 89 (12): 5577–81. June 1992. doi:10.1073/pnas.89.12.5577. PMID 1608969. Bibcode: 1992PNAS...89.5577P. 
  9. ↑ 9.0 9.1 9.2 "DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI". Mol. Cell 2 (4): 469–76. October 1998. doi:10.1016/S1097-2765(00)80146-X. PMID 9809068. http://depts.washington.edu/monnatws/pdf/1998_Jurica.pdf. 
  10. ↑ "Substrate recognition and induced DNA distortion by the PI-SceI endonuclease, an enzyme generated by protein splicing". J Mol Biol 263 (2): 163–80. October 1996. doi:10.1006/jmbi.1996.0567. PMID 8913299. 
  11. ↑ "I-PpoI and I-CreI homing site sequence degeneracy determined by random mutagenesis and sequential in vitro enrichment". J Mol Biol 280 (3): 345–53. July 1998. doi:10.1006/jmbi.1998.1886. PMID 9665841. 
  12. ↑ "Multi-site-specific endonucleases and the initiation of homologous genetic recombination in yeast". Adv Biophys 31: 77–91. 1995. doi:10.1016/0065-227X(95)99384-2. PMID 7625280. 
  13. ↑ "A group II intron RNA is a catalytic component of a DNA endonuclease involved in intron mobility". Cell 83 (4): 529–38. November 1995. doi:10.1016/0092-8674(95)90092-6. PMID 7585955. 
  14. ↑ Linn, Stuart M; Lloyd, R Stephen; Roberts, Richard J (December 1993). Nucleases. Cold Spring Harbor Press. pp. 35–88. ISBN 978-0-87969-426-5. 
  15. ↑ 15.0 15.1 Linn, Stuart M; Lloyd, R Stephen; Roberts, Richard J (December 1993). Nucleases. Cold Spring Harbor Press. pp. 89–109. ISBN 978-0-87969-426-5. 
  16. ↑ "REBASE-restriction enzymes and methylases". Nucleic Acids Res 25 (1): 248–62. January 1997. doi:10.1093/nar/25.1.248. PMID 9016548. 
  17. ↑ "Introns as mobile genetic elements". Annu Rev Biochem 62: 587–622. 1993. doi:10.1146/annurev.bi.62.070193.003103. PMID 8352597. 
  18. ↑ Linn, Stuart M; Lloyd, R Stephen; Roberts, Richard J (December 1993). Nucleases. Cold Spring Harbor Press. pp. 111–143. ISBN 978-0-87969-426-5. 
  19. ↑ Wilson GG (December 1988). "Cloned restriction-modification systems—a review". Gene 74 (1): 281–9. doi:10.1016/0378-1119(88)90304-6. PMID 3074014. 
  20. ↑ Heath, P. et al. (June 1997). "The structure of I-Crel, a group I intron-encoded homing endonuclease". Nature Structural Biology 4 (6): 468–476. doi:10.1038/nsb0697-468. PMID 9187655. 
  21. ↑ Duan, X. (May 1997). "Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity". Cell 89 (4): 555–564. doi:10.1016/S0092-8674(00)80237-8. PMID 9160747. 
  22. ↑ Van Roey, P.; Fox, KM et al. (July 2001). "Intertwined structure of the DNA-binding domain of intron endonuclease I-TevI with its substrate". EMBO J. 20 (14): 3631–3637. doi:10.1093/emboj/20.14.3631. PMID 11447104. 
  23. ↑ Van Roey, P.; Kowalski, Joseph C. et al. (July 2002). "Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI". Nature Structural Biology 9 (11): 806–811. doi:10.1038/nsb853. PMID 12379841. 
  24. ↑ Flick, K. et al. (July 1998). "DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI". Nature 394 (6688): 96–101. doi:10.1038/27952. PMID 9665136. Bibcode: 1998Natur.394...96F. 
  25. ↑ Hafez, M; Hausner, G (August 2012). "Homing endonucleases: DNA scissors on a mission.". Genome 55 (8): 553–69. doi:10.1139/g2012-049. PMID 22891613. 
  26. ↑ Shen, B.W. et al. (September 2004). "DNA binding and cleavage by the HNH homing endonuclease I-HmuI". J. Mol. Biol. 342 (1): 43–56. doi:10.1016/j.jmb.2004.07.032. PMID 15313606. 
  27. ↑ Zhao, L. et al. (May 2007). "The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif". EMBO Journal 26 (9): 2432–2442. doi:10.1038/sj.emboj.7601672. PMID 17410205. 
  28. ↑ 28.0 28.1 Steczkiewicz, K; Muszewska, A; Knizewski, L; Rychlewski, L; Ginalski, K (August 2012). "Sequence, structure and functional diversity of PD-(D/E)XK phosphodiesterase superfamily.". Nucleic Acids Research 40 (15): 7016–45. doi:10.1093/nar/gks382. PMID 22638584. 
  29. ↑ Dassa, B. et al. (March 2009). "Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family". Nucleic Acids Research 37 (8): 2560–2573. doi:10.1093/nar/gkp095. PMID 19264795. 
  30. ↑ Taylor, GK; Heiter, DF; Pietrokovski, S; Stoddard, BL (December 2011). "Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family.". Nucleic Acids Research 39 (22): 9705–19. doi:10.1093/nar/gkr669. PMID 21890897. 
  31. ↑ "Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence". Nat. Struct. Biol. 9 (10): 764–70. October 2002. doi:10.1038/nsb840. PMID 12219083. 

External links

This article incorporates text from the public domain Pfam and InterPro: IPR007868
This article incorporates text from the public domain Pfam and InterPro: IPR007869



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