Biology:m7G(5')pppN diphosphatase

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Short description: Class of enzymes
m7G(5')pppN diphosphatase
Identifiers
EC number3.6.1.30
CAS number82599-75-5
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a m7G(5')pppN diphosphatase (EC 3.6.1.30) is an enzyme that catalyzes the chemical reaction

7-methylguanosine 5'-triphospho-5'-polynucleotide + H2O [math]\displaystyle{ \rightleftharpoons }[/math] 7-methylguanosine 5'-phosphate + polynucleotide

Thus, the two substrates of this enzyme are 7-methylguanosine 5'-triphospho-5'-polynucleotide and H2O, whereas its two products are 7-methylguanosine 5'-phosphate and polynucleotide.

This is the enzyme involved in the processing of amphetamines of the cathinone group, including mephedrone and khat.

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is 7-methylguanosine-5'-triphospho-5'-polynucleotide 7-methylguanosine-5'-phosphohydrolase. Other names in common use include decapase, and m7G(5')pppN pyrophosphatase.

See also

References

  • Lavers GC (1977). "Cleavage of pm7G from mRNA 5' terminal cap structures by pyrophosphatase activity in embryonic chick lens cells". Mol. Biol. Rep. 3 (6): 413–20. doi:10.1007/BF00808382. PMID 593271. 
  • "Purification and characterization of the m7G(5')pppN-pyrophosphatase from human placenta". J. Biol. Chem. 257 (11): 6224–30. 1982. PMID 6122684. 
  • "Characterization of the m7G(5')pppN-pyrophosphatase activity from HeLa cells". J. Biol. Chem. 252 (9): 2815–21. 1977. PMID 16003.