Biology:Nanoarchaeum equitans

Short description: Species of archaeon

Nanoarchaeum equitans
Two Nanoarchaeum equitans cells (and its larger host Ignicoccus)
Scientific classification
Domain:	Archaea
Superphylum:	DPANN
Phylum:	Nanoarchaeota
Class:	Nanoarchaeia
Order:	Nanoarchaeales
Family:	Nanoarchaeaceae
Genus:	Nanoarchaeum
Species:	N. equitans
Binomial name
Nanoarchaeum equitans Huber et al. 2002

Nanoarchaeum equitans is a species of marine archaea that was discovered in 2002 in a hydrothermal vent off the coast of Iceland on the Kolbeinsey Ridge by Karl Stetter. It has been proposed as the first species in a new phylum. Strains of this microbe were also found on the Sub-polar Mid Oceanic Ridge, and in the Obsidian Pool in Yellowstone National Park. Since it grows in temperatures approaching boiling, at about 80 degrees Celsius, it is considered to be a thermophile. It grows best in environments with a pH of 6, and a salinity concentration of 2%. Nanoarchaeum appears to be an obligate symbiont on the archaeon Ignicoccus; it must be in contact with the host organism to survive. Nanoarchaeum equitans cannot synthesize lipids but obtains them from its host. Its cells are only 400 nm in diameter, making it the smallest known living organism, and the smallest known archaeon.

N. equitans' genome consists of a single circular chromosome, and has an average GC-content of 31.6%. It lacks almost all of the genes required for the synthesis of amino acids, nucleotides, cofactors, and lipids, but encodes everything needed for repair and replication. N. equitans contains several genes that encode proteins employed in recombination, suggesting that N. equitans can undergo homologous recombination. A total of 95% of its DNA encodes for proteins or stable RNA molecules.

N. equitans has small appendages that come out of its circular structure. The cell surface is covered by a thin, lattice-shaped S-layer, which provides structure and protection for the entire cell.

Genome

Mycoplasma genitalium (580 Kbp in size, with 515 protein-coding genes) was regarded as a cellular unit with the smallest genome size until 2003 when Nanoarchaeum was sequenced (491 Kbp, with 536 protein-coding genes).

Genetically, Nanoarchaeum is peculiar in that its 16S RNA sequence is undetectable by the most common methods. Initial examination of single-stranded ribosomal RNA indicated that the organism most likely belonged to the Archaea domain. However, its difference from the existing phyla, "Euryarchaeota" and Thermoproteota, was as great as the difference between the phyla. Therefore, it was given its own phylum, called "Nanoarchaeota". However, another group (see References) compared all of the open reading frames to the other Archaea. They argue that the initial sample, ribosomal RNA only, was biased and Nanoarchaeum actually belongs to the "Euryarchaeota" phylum.

The sequencing of the Nanoarchaeum genome has revealed a wealth of information about the organism's biology. The genes for several vital metabolic pathways appear to be missing. Nanoarchaeum cannot synthesize most nucleotides, amino acids, lipids, and cofactors. The cell most likely obtains these biomolecules from Ignicoccus. In particular, N. equitans lacks all of the genes that encode purine nucleotide biosynthesis in other organisms. However, unlike many parasitic microbes, Nanoarchaeum has many DNA repair enzymes, as well as everything necessary to carry out DNA replication, transcription, and translation. This may explain why the genome lacks the large stretches of non-coding DNA characteristic of other parasites.

The organism's ability to produce its own ATP is also in question.

Nanoarchaeum lacks the ability to metabolize hydrogen and sulfur for energy, as many thermophiles do. It does have five subunits of an ATP synthase as well as pathways for oxidative deamination. Whether it obtains energy from biological molecules imported from Ignicoccus, or whether it receives ATP directly is currently unknown. The genome and proteome composition of N. equitans are marked with the signatures of dual adaptation – one to high temperature and the other to obligate parasitism (or symbiosis).

See also

• Archaea
• "Candidatus Carsonella ruddii", Rickettsia, and other Pseudomonadota
• Ignicoccus
• Mycoplasma

References

• Huber, Harald (2002). "A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont". Nature 417 (6884): 63–67. doi:10.1038/417063a. PMID 11986665. Bibcode: 2002Natur.417...63H.  (This paper represents the first discovery of Nanoarchaeum.)
• Waters, Elizabeth (2003). "The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism". PNAS 100 (22): 12984–12988. doi:10.1073/pnas.1735403100. PMID 14566062. Bibcode: 2003PNAS..10012984W.  (This paper describes the genome sequence of Nanoarchaeum.)
• Brochier, Celine; Gribaldo, S; Zivanovic, Y; Confalonieri, F et al. (2005). "Nanoarchaea: representatives of a novel archaeal phylum or a fast-evolving euryarchaeal lineage related to Thermococcales?". Genome Biology 6 (5): R42. doi:10.1186/gb-2005-6-5-r42. PMID 15892870.  (Recent work suggesting that Nanoarchaeum is not a new phylum of archaea, but is a type of euryarchaeon.)
• Das, Sabyasachi (2006). "Analysis of Nanoarchaeum equitans genome and proteome composition: indications for hyperthermophilic and parasitic adaptation". BMC Genomics 7: 186. doi:10.1186/1471-2164-7-186. PMID 16869956.  (This paper describes the genome and proteome analysis of Nanoarchaeum.)

Further reading

Di Giulio, Massimo (January 1, 2013). "Is Nanoarchaeum equitans a paleokaryote?". Journal of Biological Research. 

External links

• NCBI taxonomy page for Nanoarchaeota
• Tree of Life Nanoarchaeota
• LSPN page for Nanoarchaeota
• MicrobeWiki page for Nanoarchaeum

Wikidata ☰ Q221936 entry