Biology:Thiamine-phosphate diphosphorylase
| thiamin-phosphate diphosphorylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.5.1.3 | ||||||||
| CAS number | 9030-30-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a thiamine-phosphate diphosphorylase ( or, thiamine-phosphate pyrophosphorylase ) (EC 2.5.1.3) is an enzyme that catalyzes the chemical reaction
- 4-Amino-5-hydroxymethyl-2-methylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole [math]\displaystyle{ \rightleftharpoons }[/math] diphosphate + thiamine monophosphate
The two substrates of this enzyme are 4-Amino-5-hydroxymethyl-2-methylpyrimidine diphosphate and 4-methyl-5-(2-phosphono-oxyethyl)thiazole; its two products are diphosphate and thiamine monophosphate.
This enzyme belongs to the family of transferases, specifically those transferring aryl or alkyl groups other than methyl groups. This enzyme is on the biosynthetic pathway to thiamine.[1][2]
Nomenclature
The systematic name of this enzyme class is 2-methyl-4-amino-5-hydroxymethylpyrimidine-diphosphate:4-methyl-5-(2 -phosphoethyl)thiazole 2-methyl-4-aminopyrimidine-5-methenyltransferase. Other names in common use include
- thiamine phosphate synthase,
- thiamine phosphate pyrophosphorylase,
- thiamine monophosphate pyrophosphorylase, and
- TMP-PPase.
Structural studies
As of late 2007, 9 structures have been solved for this class of enzymes, with PDB accession codes 1G4E, 1G4P, 1G4S, 1G4T, 1G67, 1G69, 1G6C, 1XI3, and 2TPS.
References
- ↑ "Pathway: superpathway of thiamine diphosphate biosynthesis I". MetaCyc Metabolic Pathway Database. 2011-09-14. https://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=THISYN-PWY&detail-level=2#.
- ↑ "Determination of the genetic, molecular, and biochemical basis of the Arabidopsis thaliana thiamin auxotroph th1". Archives of Biochemistry and Biophysics 459 (1): 107–114. March 2007. doi:10.1016/j.abb.2006.11.011. PMID 17174261.
Further reading
- "The biosynthesis of thiamine. 2. Fractionation of enzyme system and identification of thiazole monophosphate and thiamine monophosphate as intermediates". The Journal of Biological Chemistry 235: 2411–2417. August 1960. PMID 13807175.
- "The enzymatic synthesis of thiamine monophosphate". The Journal of Biological Chemistry 236: 3066–3071. November 1961. PMID 14463407.
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