Biology:List of restriction enzyme cutting sites: L–N
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Revision as of 10:18, 12 August 2022 by imported>JTerm (correction)
Short description: None
Legend of nucleobases | |
---|---|
Code | Nucleotide represented |
A | Adenine (A) |
C | Cytosine (C) |
G | Guanine (G) |
T | Thymine (T) |
N | A, C, G or T |
M | A or C |
R | A or G |
W | A or T |
Y | C or T |
S | C or G |
K | G or T |
H | A, C or T |
B | C, G or T |
V | A, C or G |
D | A, G or T |
This article contains a list of the most studied restriction enzymes whose names start with L to N inclusive. It contains approximately 120 enzymes.
The following information is given:
- Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
- PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
- Source: Organism that naturally produces the enzyme.
- Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
- Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
- Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme: EcoR70I
Restriction enzymes
L
Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers | ||||||
LcaI | Lactobacillus casei | 5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
BdiI, Bli41I, Bsa29I, BscI, Bsp106I, BspDI, Bst28I, LplI, PgaI, ZhoI | |||||||
LlaAI | Lactococcus lactis cremoris W9 | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bme12I, Bsp67I, BstENII, ChaI, FnuEI, MgoI, NphI, RalF40I | |||||||
LlaBI | Lactococcus lactis cremoris W56 | 5' CTRYAG 3' GAYRTC
|
5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5'
|
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LlaCI | Lactococcus lactis W15 | 5' AAGCTT 3' TTCGAA
|
5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5'
|
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LlaG2I | Lactococcus lactis cremoris | 5' GCTAGC 3' CGATCG
|
5' ---G CTAGC--- 3' 3' ---CGATC G--- 5'
|
| |||||||
Lmu60I | Listeria murrayi 60 | 5' CCTNAGG 3' GGANTCC
|
5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5'
|
AocI, AxyI, BspR7I, CvnI, Lmu60I, MstII, SauI, SshAI | |||||||
LplI | Lactobacillus plantarum | 5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
BanIII, BbvAII, BdiI, Bsa29I, BscI, BstNZ169I, ClaI, LcaI, PgaI | |||||||
LpnI | Legionella pneumophila | 5' RGCGCY 3' YCGCGR
|
5' ---RGC GCY--- 3' 3' ---YCG CGR--- 5'
|
| |||||||
LspI | Lactobacillus sp. | 5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
Asp10HI, BimI, BsiCI, BstBI, Csp45I, PlaII, PpaAI, Ssp1I | |||||||
LweI | Listeria welshimeri RFL131 | 5' GCATC 3' CGTAG
|
5' ---GCATCN4N NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5'
|
M
Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers | ||||||
MabI | Microbacterium arborescens SE | 5' ACCWGGT 3' TGGWCCA
|
5' ---A CCWGGT--- 3' 3' ---TGGWCC A--- 5'
|
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MaeI | Methanococcus aeolicus PL-15/H | 5' CTAG 3' GATC
|
5' ---C TAG--- 3' 3' ---GAT C--- 5'
|
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MaeII | Methanococcus aeolicus | 5' ACGT 3' TGCA
|
5' ---A CGT--- 3' 3' ---TGC A--- 5'
|
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MaeIII | Methanococcus aeolicus | 5' GTNAC 3' CANTG
|
5' --- GTNAC--- 3' 3' ---CANTG --- 5'
|
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MaeK81I | Microcystis aeruginosa K-81 | 5' CGTACG 3' GCATGC
|
5' ---C GTACG--- 3' 3' ---GCATG C--- 5'
|
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MaeK81II | Microcystis aeruginosa | 5' GGNCC 3' CCNGG
|
5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
|
AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, Sau96I | |||||||
MamI | Microbacterium ammoniaphilum | 5' GATN4ATC 3' CTAN4TAG
|
5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5'
|
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MavI | Mycobacterium avium | 5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SciI, SlaI, Sol10179I, XhoI | |||||||
MbiI | Moraxella bovis Fr1-022 | 5' CCGCTC 3' GGCGAG
|
5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5'
|
AccBSI, BsrBI, BstD102I, Bst31NI | |||||||
MboI | Moraxella bovis | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
AspMDI, Bsp105I, BspFI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I | |||||||
MboII | Moraxella bovis | 5' GAAGA 3' CTTCT
|
5' ---GAAGAN6NN --- 3' 3' ---CTTCTN6N N--- 5'
|
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MchI | Micromonospora chalcea | 5' GGCGCC 3' CCGCGG
|
5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5'
|
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MchAI | Mycobacterium chelonae | 5' GCGGCCGC 3' CGCCGGCG
|
5' ---GC GGCCGC--- 3' 3' ---CGCCGG CG--- 5'
|
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MchAII | Mycobacterium chelonae | 5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
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McrI | Micrococcus cryophilus | 5' CGRYCG 3' GCYRGC
|
5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5'
|
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MfeI | Mycoplasma fermentans | 5' CAATTG 3' GTTAAC
|
5' ---C AATTG--- 3' 3' ---GTTAA C--- 5'
|
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MflI | Microbacterium flavum | 5' RGATCY 3' YCTAGR
|
5' ---R GATCY--- 3' 3' ---YCTAG R--- 5'
|
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MfoAI | Mycobacterium fortuitum TMC 1529 | 5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
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Mgl14481I | Merismopedia glauca 1448-1 | 5' CCSGG 3' GGSCC
|
5' ---CC SGG--- 3' 3' ---GGS CC--- 5'
|
AseII, AsuC2I, BcnI, BpuMI, CauII, Eco1831I, EcoHI, HgiS22I, NciI | |||||||
MgoI | Mycobacterium gordonae | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
AspMDI, BspAI, BstENII, ChaI, FnuEI, MkrAI, NphI, Sau3AI | |||||||
MhaAI | Mycobacterium habana 206 | 5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
AliAJI, Asp713I, Bsp63I, CstI, Ecl37kI, HalII, PaePI, PstI, YenI | |||||||
MhlI | Micrococcus halobius SD | 5' GDGCHC 3' CHCGDG
|
5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5'
|
AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, SduI | |||||||
MkrAI | Micrococcus kristinae | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bme12I, BspAI, BstENII, ChaI, FnuEI, MgoI, NphI, Sau3AI | |||||||
MlaI | Mastigocladus laminosus | 5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
Asp10HI, BimI, BsiCI, BstBI, Csp68KII, LspI, PlaII, Ssp1I | |||||||
MlaAI | Micromonospora lacustris | 5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
BspAAI, BstVI, PanI, SauLPII, Sbi68I, Sfr274I, SlaI, XhoI | |||||||
MlsI | Micrococcus luteus Ng 16-122 | 5' TGGCCA 3' ACCGGT
|
5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5'
|
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MltI | Micrococcus luteus | 5' AGCT 3' TCGA
|
5' ---AG CT--- 3' 3' ---TC GA--- 5'
|
AluBI, AluI | |||||||
MluI | Micrococcus luteus | 5' ACGCGT 3' TGCGCA
|
5' ---A CGCGT--- 3' 3' ---TGCGC A--- 5'
|
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Mlu23I | Micrococcus luteus 23 | 5' GGATCC 3' CCTAGG
|
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
|
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Mlu31I | Micrococcus luteus 31 | 5' TGGCCA 3' ACCGGT
|
5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5'
|
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MluB2I | Micrococcus luteus B2 | 5' TCGCGA 3' AGCGCT
|
5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5'
|
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MluNI | Micrococcus luteus N | 5' TGGCCA 3' ACCGGT
|
5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5'
|
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MlyI | Micrococcus lylae | 5' GAGTC 3' CTCAG
|
5' ---GAGTCN4N --- 3' 3' ---CTCAGN4N --- 5'
|
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Mly113I | Micrococcus lylae 113 | 5' GGCGCC 3' CCGCGG
|
5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5'
|
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MmeI | Methylophilus methylotrophus | 5' TCCRAC 3' AGGYTG
|
5' ---TCCRACN17NNN --- 3' 3' ---AGGYTGN17N NN--- 5'
|
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MnlI | Moraxella nonliquefaciens | 5' CCTC 3' GGAG
|
5' ---CCTCN4NNN --- 3' 3' ---GGAGN4NN N--- 5'
|
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MnoI | Moraxella nonliquefaciens | 5' CCGG 3' GGCC
|
5' ---C CGG--- 3' 3' ---GGC C--- 5'
|
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Mph1103I | Moraxella phenylpyruvica RFL1103 | 5' ATGCAT 3' TACGTA
|
5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5'
|
BfrBI, Csp68KIII, EcoT22I, NsiI, PinBI, Ppu10I, SepI, SspD5II | |||||||
MroI | Micrococcus roseus | 5' TCCGGA 3' AGGCCT
|
5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5'
|
BbvAIII, BseAI, Bsp13I, BspEI, Bsu23I, CauB3I, PinBII, PtaI | |||||||
MroNI | Micrococcus roseus N | 5' GCCGGC 3' CGGCCG
|
5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5'
|
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MroXI | Micrococcus roseus XM | 5' GAAN4TTC 3' CTTN4AAG
|
5' ---GAANN NNTTC--- 3' 3' ---CTTNN NNAAG--- 5'
|
Asp700I, BbvAI, PdmI, XmnI | |||||||
MscI | Micrococcus sp. | 5' TGGCCA 3' ACCGGT
|
5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5'
|
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MseI | Micrococcus sp. | 5' TTAA 3' AATT
|
5' ---T TAA--- 3' 3' ---AAT T--- 5'
|
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MslI | Moraxella osloensis | 5' CAYN4RTG 3' GTRN4YAC
|
5' ---CAYNN NNRTG--- 3' 3' ---GTRNN NNYAC--- 5'
|
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MspI | 1SA3, 1YFI, Search PDBe | Moraxella sp. | 5' CCGG 3' GGCC
|
5' ---C CGG--- 3' 3' ---GGC C--- 5'
|
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Msp17I | Micrococcus sp. 17 | 5' GRCGYC 3' CYGCRG
|
5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5'
|
AsuIII, BsaHI, BstACI, HgiGI, HgiHII, Hin1I, Msp17I, PamII | |||||||
Msp20I | Micrococcus sp. | 5' TGGCCA 3' ACCGGT
|
5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5'
|
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Msp67I | Micrococcus sp. MS67 | 5' CCNGG 3' GGNCC
|
5' ---CC NGG--- 3' 3' ---GGN CC--- 5'
|
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MspA1I | Moraxella sp. A1 | 5' CMGCKG 3' GKCGMC
|
5' ---CMG CKG--- 3' 3' ---GKC GMC--- 5'
|
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MspB4I | Moraxella sp. B4 | 5' GGYRCC 3' CCRYGG
|
5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5'
|
AccB1I, BanI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, PfaAI | |||||||
MspCI | Micrococcus sp. | 5' CTTAAG 3' GAATTC
|
5' ---C TTAAG--- 3' 3' ---GAATT C--- 5'
|
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MspR9I | Micrococcus sp. R9 | 5' CCNGG 3' GGNCC
|
5' ---CC NGG--- 3' 3' ---GGN CC--- 5'
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MspSWI | Moraxella sp. SW | 5' ATTTAAAT 3' TAAATTTA
|
5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5'
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MspV281I | Myxosarcinia sp. V/281 | 5' GWGCWC 3' CWCGWG
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5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5'
|
| |||||||
MspYI | Microcystis sp. J2 | 5' YACGTR 3' RTGCAY
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5' ---YAC GTR--- 3' 3' ---RTG CAY--- 5'
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MssI | Methylobacterium sp. Dd 5-732 | 5' GTTTAAAC 3' CAAATTTG
|
5' ---GTTT AAAC--- 3' 3' ---CAAA TTTG--- 5'
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PmeI | |||||||
MstI | Microcoleus sp. | 5' TGCGCA 3' ACGCGT
|
5' ---TGC GCA--- 3' 3' ---ACG CGT--- 5'
|
Acc16I, AosI, AviII, FdiII, FspI, NsbI, PamI, Pun14627I | |||||||
MstII | Microcoleus sp. | 5' CCTNAGG 3' GGANTCC
|
5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5'
|
AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI | |||||||
MthZI | Methanobacterium thermoformicicum Z-245 | 5' CTAG 3' GATC
|
5' ---C TAG--- 3' 3' ---GAT C--- 5'
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MunI | 1D02 | Mycoplasma sp. | 5' CAATTG 3' GTTAAC
|
5' ---C AATTG--- 3' 3' ---GTTAA C--- 5'
|
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MvaI | 2OAA, 2OA9, Search PDBe, 2OA9 | Micrococcus varians RFL19 | 5' CCWGG 3' GGWCC
|
5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
|
AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, Bst2UI, SslI, TaqXI, ZanI | ||||||
Mva1269I | Micrococcus varians RFL1269 | 5' GAATGC 3' CTTACG
|
5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5'
|
Asp26HI, Asp40HI, Asp50HI, BscCI, BsmI, Mva1269I, PctI | |||||||
MvnI | Methanococcus vannielii | 5' CGCG 3' GCGC
|
5' ---CG CG--- 3' 3' ---GC GC--- 5'
|
AccII, Bsh1236I, BstFNI, BtkI, Csp68KVI, FalII, FauBII, ThaI | |||||||
MvrI | Micrococcus varians | 5' CGATCG 3' GCTAGC
|
5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5'
|
Afa22MI, BspCI, EagBI, ErhB9I, Ple19I, PvuI, RshI, XorII | |||||||
MwoI | Methanobacterium wolfei | 5' GCN7GC 3' CGN7CG
|
5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5'
|
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MxaI | Myxococcus xanthus F18E | 5' GAGCTC 3' CTCGAG
|
5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5'
|
N
Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers | ||||||
NaeI | 1EV7, 1iaw, Search PDB | Nocardia aerocolonigenes | 5' GCCGGC 3' CGGCCG
|
5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'
|
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NarI | Nocardia argentinensis | 5' GGCGCC 3' CCGCGG
|
5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5'
|
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NblI | Nocardia blackwellii | 5' CGATCG 3' GCTAGC
|
5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5'
|
Afa16RI, Afa22MI, EagBI, MvrI, Ple19I, Psu161I, RshI, XorII | |||||||
NciI | Neisseria cinerea | 5' CCSGG 3' GGSCC
|
5' ---CC SGG--- 3' 3' ---GGS CC--- 5'
|
| |||||||
NcoI | Nocardia corallina | 5' CCATGG 3' GGTACC
|
5' ---C CATGG--- 3' 3' ---GGTAC C--- 5'
|
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NcrI | Nocardia carnea C-212 | 5' AGATCT 3' TCTAGA
|
5' ---A GATCT--- 3' 3' ---TCTAG A--- 5'
|
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NcuI | Neisseria cuniculi | 5' GAAGA 3' CTTCT
|
5' ---GAAGAN6NN --- 3' 3' ---CTTCTN6N N--- 5'
|
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NdaI | Nocardia dassonvillei | 5' GGCGCC 3' CCGCGG
|
5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5'
|
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NdeI | Neisseria denitrificans | 5' CATATG 3' GTATAC
|
5' ---CA TATG--- 3' 3' ---GTAT AC--- 5'
|
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NdeII | Neisseria denitrificans | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bce243I, Bsp105I, BspJI, BstMBI, CviAI, Kzo9I, NlaII, Sth368I | |||||||
NgoAIII | Neisseria gonorrhoeae FA1090 | 5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
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NgoAIV | Neisseria gonorrhoeae FA1090 | 5' GCCGGC 3' CGGCCG
|
5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5'
|
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NgoMIV | 1FIU, 4abt Search PDBe θ | Neisseria gonorrhoeae MS11 | 5' GCCGGC 3' CGGCCG
|
5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5'
|
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NgoPII | Neisseria gonorrhoeae P9-2 | 5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
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NgoPIII | Neisseria gonorrhoeae P9-2 | 5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
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NheI | Neisseria mucosa heidelbergensis | 5' GCTAGC 3' CGATCG
|
5' ---G CTAGC--- 3' 3' ---CGATC G--- 5'
|
| |||||||
NlaII | Neisseria lactamica | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
BscFI, BspAI, BstENII, ChaI, FnuEI, MgoI, RalF40I, Sau3AI | |||||||
NlaIII | Neisseria lactamica | 5' CATG 3' GTAC
|
5' ---CATG --- 3' 3' --- GTAC--- 5'
|
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NlaIV | Neisseria lactamica | 5' GGNNCC 3' CCNNGG
|
5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5'
|
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Nli3877I | Nostoc linckia | 5' CYCGRG 3' GRGCYC
|
5' ---CYCGR G--- 3' 3' ---G RGCYC--- 5'
|
Ama87I, AvaI, BcoI, BsiHKCI, Eco27kI, Eco88I, NspSAI, OfoI | |||||||
NmeCI | Neisseria meningitidis C114 | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
BscFI, BspAI, BstENII, ChaI, FnuEI, MgoI, RalF40I, Sau3AI | |||||||
NmeRI | Neisseria meningitidis | 5' CAGCTG 3' GTCGAC
|
5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
|
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NmuCI | Neisseria mucosa C9-2 | 5' GTSAC 3' CASTG
|
5' --- GTSAC--- 3' 3' ---CASTG --- 5'
|
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NopI | Nocardia opaca | 5' GTCGAC 3' CAGCTG
|
5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5'
|
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NotI | 3C25, 3BVQ, Search PDBe | Nocardia otitidis-caviarum | 5' GCGGCCGC 3' CGCCGGCG
|
5' ---GC GGCCGC--- 3' 3' ---CGCCGG CG--- 5'
|
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NphI | Neisseria pharyngis C245 | 5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
AspMDI, BspAI, BstENII, ChaI, FnuEI, MkrAI, RalF40I, Sau3AI | |||||||
NruI | Nocardia rubra | 5' TCGCGA 3' AGCGCT
|
5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5'
|
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NruGI | Nocardia sp. G | 5' GACN5GTC 3' CTGN5CAG
|
5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5'
|
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NsbI | Neisseria subflava Va-1 | 5' TGCGCA 3' ACGCGT
|
5' ---TGC GCA--- 3' 3' ---ACG CGT--- 5'
|
Acc16I, AosI, AviII, FdiII, FspI, MstI, PamI, Pun14627I | |||||||
NsiI | Neisseria sicca | 5' ATGCAT 3' TACGTA
|
5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5'
|
BfrBI, Csp68KIII, EcoT22I, PinBI, Ppu10I, SepI, SspD5II, Zsp2I | |||||||
NsiCI | Neisseria sicca C351 | 5' GATATC 3' CTATAG
|
5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5'
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NspI | Nostoc sp. C | 5' RCATGY 3' YGTACR
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5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5'
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NspII | Nostoc sp. C | 5' GDGCHC 3' CHCGDG
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5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5'
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AocII, BmyI, BsoCI, Bsp1286I, MhlI, NspII, SduI | |||||||
NspIII | Nostoc sp. C | 5' CYCGRG 3' GRGCYC
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5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5'
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Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, Eco88I, OfoI, PunAI | |||||||
NspIV | Nostoc sp. C | 5' GGNCC 3' CCNGG
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5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
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AvcI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI | |||||||
NspV | Nostoc sp. C | 5' TTCGAA 3' AAGCTT
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5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
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Asp10HI, BimI, Bsp119I, BstBI, Csp68KII, MlaI, PlaII, SspRFI | |||||||
Nsp7121I | Nostoc sp. 7121 | 5' GGNCC 3' CCNGG
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5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
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AvcI, Bal228I, Bce22I, Bsp1894I, Bsu54I, Cfr13I, NspIV, UnbI | |||||||
Nsp29132II | Nostoc sp. | 5' GGATCC 3' CCTAGG
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5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
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AliI, ApaCI, BamHI, Bce751I, Nsp29132II, Pfl8I, SolI, Uba4009I | |||||||
NspBII | Nostoc sp. | 5' CMGCKG 3' GKCGMC
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5' ---CMG CKG--- 3' 3' ---GKC GMC--- 5'
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NspHI | Nostoc sp. | 5' RCATGY 3' YGTACR
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5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5'
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NspLKI | Nocardia sp. LK | 5' GGCC 3' CCGG
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5' ---GG CC--- 3' 3' ---CC GG--- 5'
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NspMACI | Nostoc sp. | 5' AGATCT 3' TCTAGA
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5' ---A GATCT--- 3' 3' ---TCTAG A--- 5'
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NspSAI | Nostoc sp. SA | 5' CYCGRG 3' GRGCYC
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5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5'
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Ama87I, AvaI, Bse15I, BspLU4I, Eco27kI, Nli3877I, OfoI, PunAI | |||||||
NspSAII | Nostoc sp. SA | 5' GGTNACC 3' CCANTGG
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5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
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AspAI, Bse64I, BseT9I, BseT10I, BstPI, Eco91I, NspSAII, PspEI | |||||||
NspSAIV | Nostoc sp. SA | 5' GGATCC 3' CCTAGG
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5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
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AliI, BamHI, Bsp98I, Bsp4009I, NspSAIV, Pfl8I, SolI, Uba4009I | |||||||
NunII | Nocardia uniformis | 5' GGCGCC 3' CCGCGG
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5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5'
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Notes
- ↑ "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. December 1973. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
- ↑ "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. April 2003. doi:10.1093/nar/gkg274. PMID 12654995.
- ↑ "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. 2002. ISBN 0-7167-4684-0. https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=books&doptcmdl=GenBookHL&term=stryer%5Bbook%5D+AND+215168%5Buid%5D&rid=stryer.chapter.280.
- ↑ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
- ↑ "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene 92 (1-2): 1–248. August 1990. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
- ↑ "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. September 2001. doi:10.1093/nar/29.18.3705. PMID 11557805.
Original source: https://en.wikipedia.org/wiki/List of restriction enzyme cutting sites: L–N.
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