Biology:7alpha-hydroxysteroid dehydrogenase
| 7-alpha-hydroxysteroid dehydrogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.1.1.159 | ||||||||
| CAS number | 39361-64-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a 7alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) is an enzyme that catalyzes the chemical reaction
- 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + NAD+ [math]\displaystyle{ \rightleftharpoons }[/math] 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
Thus, the two substrates of this enzyme are 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate and NAD+, whereas its 3 products are 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate, NADH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 7alpha-hydroxysteroid:NAD+ 7-oxidoreductase. Other names in common use include 7alpha-hydroxy steroid dehydrogenase, and 7alpha-HSDH.
Structural studies
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1AHH, 1AHI, and 1FMC.
References
- "The specificity of a 7 alpha-hydroxy steroid dehydrogenase from Escherichia coli". The Biochemical Journal 157 (1): 207–10. July 1976. doi:10.1042/bj1570207. PMID 786279.
- "Bile induction of 7 alpha- and 7 beta-hydroxysteroid dehydrogenases in Clostridium absonum". Biochimica et Biophysica Acta 665 (2): 262–9. August 1981. doi:10.1016/0005-2760(81)90011-4. PMID 6945134.
- "7Alpha-hydroxysteroid dehydrogenase from Escherichia coli B: preliminary studies". Biochimica et Biophysica Acta (BBA) - Enzymology 309 (2): 243–53. June 1973. doi:10.1016/0005-2744(73)90022-3. PMID 4581498.
- "NAD- and NADP-dependent 7alpha-hydroxysteroid dehydrogenases from bacteroides fragilis". Biochimica et Biophysica Acta (BBA) - Enzymology 384 (1): 12–24. March 1975. doi:10.1016/0005-2744(75)90091-1. PMID 236764.
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