- 3-hydroxybenzoate + NADPH + H+ + O2 [math]\rightleftharpoons[/math] 3,4-dihydroxybenzoate + NADP+ + H2O
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is 3-hydroxybenzoate,NADPH:oxygen oxidoreductase (4-hydroxylating). This enzyme is also called 3-hydroxybenzoate 4-hydroxylase. This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, FAD.
- "3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni". Biochem. Biophys. Res. Commun. 55 (3): 888–96. 1973. doi:10.1016/0006-291X(73)91227-8. PMID 4148586.
- "m-Hydroxybenzoic acid 4-hydroxylase from Aspergillus niger". Can. J. Biochem. 47 (8): 825–7. 1969. doi:10.1139/o69-129. PMID 4390252.
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