Biology:3-hydroxybenzoate 4-monooxygenase

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In enzymology, a 3-hydroxybenzoate 4-monooxygenase (EC is an enzyme that catalyzes the chemical reaction

3-hydroxybenzoate + NADPH + H+ + O2 [math]\rightleftharpoons[/math] 3,4-dihydroxybenzoate + NADP+ + H2O

The 4 substrates of this enzyme are 3-hydroxybenzoate, NADPH, H+, and O2, whereas its 3 products are 3,4-dihydroxybenzoate, NADP+, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is 3-hydroxybenzoate,NADPH:oxygen oxidoreductase (4-hydroxylating). This enzyme is also called 3-hydroxybenzoate 4-hydroxylase. This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, FAD.

Structural studies[edit]

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2DKH and 2DKI.

References[edit] 4-monooxygenase was the original source. Read more.