Biology:4-hydroxyphenylacetaldehyde oxime monooxygenase
| 4-hydroxyphenylacetaldehyde oxime monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.13.68 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a 4-hydroxyphenylacetaldehyde oxime monooxygenase (EC 1.14.13.68) is an enzyme that catalyzes the chemical reaction
- (Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + H+ + O2 [math]\displaystyle{ \rightleftharpoons }[/math] (S)-4-hydroxymandelonitrile + NADP+ + 2 H2O
The 4 substrates of this enzyme are (Z)-4-hydroxyphenylacetaldehyde oxime, NADPH, H+, and O2, whereas its 3 products are (S)-4-hydroxymandelonitrile, NADP+, and H2O.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is (Z)-4-hydroxyphenylacetaldehyde oxime,NADPH:oxygen oxidoreductase. Other names in common use include 4-hydroxybenzeneacetaldehyde oxime monooxygenase, cytochrome P450II-dependent monooxygenase, NADPH-cytochrome P450 reductase (CYP71E1), CYP71E1, and 4-hydroxyphenylacetaldehyde oxime,NADPH:oxygen oxidoreductase. This enzyme participates in tyrosine metabolism.
References
- "The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor". J. Biol. Chem. 250 (12): 4708–13. 1975. doi:10.1016/S0021-9258(19)41359-8. PMID 237909.
- "The enzymatic conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile". Arch. Biochem. Biophys. 180 (1): 199–207. 1977. doi:10.1016/0003-9861(77)90026-1. PMID 193443.
- "Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants". Plant Physiol. 129 (3): 1222–31. 2002. doi:10.1104/pp.000687. PMID 12114576.
- BL, Bak S; Morant, M; Olsen, CE; Ekstrøm, CT; Galbraith, DW; Møller, BL; Bak, S (2005). "Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome". Proc. Natl. Acad. Sci. U.S.A. 102 (5): 1779–84. doi:10.1073/pnas.0409233102. PMID 15665094. Bibcode: 2005PNAS..102.1779K.
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