Biology:Ammonia monooxygenase
From HandWiki
| Ammonia monooxygenase | |||||||||
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| Identifiers | |||||||||
| EC number | 1.14.99.39 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Ammonia monooxygenase (EC 1.14.99.39, AMO) is an enzyme,[1][2][3][4][5][6][7][8][9] which catalyses the following chemical reaction
- ammonia + AH2 + O2 [math]\displaystyle{ \rightleftharpoons }[/math] NH2OH + A + H2O
Ammonia monooxygenase contains copper and possibly nonheme iron. AMO is the first enzyme in ammonia oxidation. Aerobic oxidation of ammonia to hydroxylamine via AMO is an endergonic reaction. So, all aerobic ammonia oxidizing organisms conserve energy by further oxidizing hydroxylamine. It was believed that aerobic ammonia-oxidizing bacteria oxidize hydroxylamine to nitrite using octahaem hydroxylamine oxidoreductase (HAO). Recently, it was shown that the product of HAO is not nitrite but nitric oxide, which is further oxidized to nitrite by an unknown enzyme.
References
- ↑ "Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea". Applied and Environmental Microbiology 60 (8): 3033–5. August 1994. doi:10.1128/AEM.60.8.3033-3035.1994. PMID 8085841.
- ↑ "Sequence of the gene, amoB, for the 43-kDa polypeptide of ammonia monoxygenase of Nitrosomonas europaea". Biochemical and Biophysical Research Communications 204 (2): 759–62. October 1994. doi:10.1006/bbrc.1994.2524. PMID 7980540.
- ↑ "Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related". FEMS Microbiology Letters 132 (3): 203–8. October 1995. doi:10.1111/j.1574-6968.1995.tb07834.x. PMID 7590173.
- ↑ "Evidence for an iron center in the ammonia monooxygenase from Nitrosomonas europaea". FEBS Letters 397 (1): 35–8. November 1996. doi:10.1016/s0014-5793(96)01116-7. PMID 8941709.
- ↑ "The purification of ammonia monooxygenase from Paracoccus denitrificans". FEBS Letters 387 (1): 71–4. May 1996. doi:10.1016/0014-5793(96)00463-2. PMID 8654570.
- ↑ "Electron transfer during the oxidation of ammonia by the chemolithotrophic bacterium Nitrosomonas europaea". Biochimica et Biophysica Acta (BBA) - Bioenergetics 1459 (2–3): 346–55. August 2000. doi:10.1016/s0005-2728(00)00171-7. PMID 11004450.
- ↑ "Molecular biology and biochemistry of ammonia oxidation by Nitrosomonas europaea". Archives of Microbiology 178 (4): 250–5. October 2002. doi:10.1007/s00203-002-0452-0. PMID 12209257.
- ↑ "A soluble form of ammonia monooxygenase in Nitrosomonas europaea". Biological Chemistry 390 (9): 863–73. September 2009. doi:10.1515/BC.2009.085. PMID 19453274.
- ↑ "Oxidation of monohalogenated ethanes and n-chlorinated alkanes by whole cells of Nitrosomonas europaea". Journal of Bacteriology 172 (9): 5368–73. September 1990. doi:10.1128/jb.172.9.5368-5373.1990. PMID 2394686.
External links
- Ammonia+monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)
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