Biology:Beta-galactoside alpha-2,6-sialyltransferase

From HandWiki
Short description: Enzyme
beta-galactoside alpha-2,6-sialyltransferase
Identifiers
EC number2.4.99.1
CAS number9075-81-4
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a beta-galactoside alpha-2,6-sialyltransferase (EC 2.4.99.1) is an enzyme that catalyzes the chemical reaction

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine [math]\displaystyle{ \rightleftharpoons }[/math] CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D- glucosamine

Thus, the two substrates of this enzyme are CMP-N-acetylneuraminate and beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine, whereas its three products are CMP, alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-, and glucosamine.

This enzyme belongs to the family of transferases, specifically those glycosyltransferases that do not transfer hexosyl or pentosyl groups. The systematic name of this enzyme class is CMP-N-acetylneuraminate:beta-D-galactosyl-1,4-N-acetyl-beta-D-glucos amine alpha-2,6-N-acetylneuraminyltransferase. This enzyme participates in n-glycan biosynthesis and glycan structures - biosynthesis 1.

References

  • "The sialic acids. XV. Transfer of sialic acid to glycoproteins by a sialyltransferase from colostrum". J. Biol. Chem. 248 (16): 5751–62. 1973. PMID 4723915. 
  • "Physical and chemical studies on ceruloplasmin. 8. Preparation of N-acetylneuraminic acid-1-14C-labeled ceruloplasmin". J. Biol. Chem. 245 (4): 759–66. 1970. PMID 4313609. 
  • "Purification of a sialyltransferase from bovine colostrum by affinity chromatography on CDP-agarose". J. Biol. Chem. 252 (7): 2356–62. 1977. PMID 849932. 
  • "Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type". Methods Enzymol. 98: 98–134. 1983. doi:10.1016/0076-6879(83)98143-0. PMID 6366476. 
  • "Glycoprotein biosynthesis: studies on thyroglobulin. Thyroid sialyltransferase". J. Biol. Chem. 243 (24): 6520–8. 1968. PMID 5726897.