Biology:Pyrimidine-deoxynucleoside 1'-dioxygenase

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pyrimidine-deoxynucleoside 1'-dioxygenase
Identifiers
EC number1.14.11.10
CAS number98865-52-2
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a pyrimidine-deoxynucleoside 1'-dioxygenase (EC 1.14.11.10) is an enzyme that catalyzes the chemical reaction

2'-deoxyuridine + 2-oxoglutarate + O2 [math]\displaystyle{ \rightleftharpoons }[/math] uracil + 2-deoxyribonolactone + succinate + CO2

The 3 substrates of this enzyme are 2'-deoxyuridine, 2-oxoglutarate, and O2, whereas its 4 products are uracil, 2-deoxyribonolactone, succinate, and CO2.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with 2-oxoglutarate as one donor, and incorporation of one atom o oxygen into each donor. The systematic name of this enzyme class is 2'-deoxyuridine,2-oxoglutarate:oxygen oxidoreductase (1'-hydroxylating). This enzyme is also called deoxyuridine-uridine 1'-dioxygenase. It has 2 cofactors: iron, and Ascorbate.

References

  • "Identification of two alpha-ketoglutarate-dependent dioxygenases in extracts of Rhodotorula glutinis catalyzing deoxyuridine hydroxylation". The Journal of Biological Chemistry 260 (18): 9972–5. August 1985. PMID 4040518.