Biology:FYVE domain
| FYVE zinc finger | |||||||||
|---|---|---|---|---|---|---|---|---|---|
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| Identifiers | |||||||||
| Symbol | FYVE | ||||||||
| Pfam | PF01363 | ||||||||
| InterPro | IPR000306 | ||||||||
| PROSITE | PDOC50178 | ||||||||
| SCOP2 | 1vfy / SCOPe / SUPFAM | ||||||||
| OPM superfamily | 59 | ||||||||
| OPM protein | 1vfy | ||||||||
| CDD | cd00065 | ||||||||
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In molecular biology the FYVE zinc finger domain is named after the four cysteine-rich proteins: Fab 1 (yeast orthologue of PIKfyve), YOTB, Vac 1 (vesicle transport protein), and EEA1, in which it has been found. FYVE domains bind phosphatidylinositol 3-phosphate,[2] in a way dependent on its metal ion coordination and basic amino acids. The FYVE domain inserts into cell membranes in a pH-dependent manner.[3] The FYVE domain has been connected to vacuolar protein sorting and endosome function.[4]
Structure
The FYVE domain is composed of two small beta hairpins (or zinc knuckles) followed by an alpha helix.[5] The FYVE finger binds two zinc ions. The FYVE finger has eight potential zinc coordinating cysteine positions and is characterized by having basic amino acids around the cysteines. Many members of this family also include two histidines in a sequence motif:
The FYVE finger is structurally similar to the RING domain and the PHD finger.
Examples
The following is a list of human proteins containing this domain:
- ANKFY1, EEA1, FGD1, FGD2, FGD3, FGD4, FGD5, FGD6, FYCO1, HGS, MTMR3, MTMR4, PIKFYVE, PLEKHF1, PLEKHF2
- RUFY1, RUFY2, RUFY3, RUFY4, WDFY1, WDFY2, WDFY3, ZFYVE1, ZFYVE9, ZFYVE16, ZFYVE19, ZFYVE20, ZFYVE21, ZFYVE26, ZFYVE27, ZFYVE28
References
- ↑ "Multivalent endosome targeting by homodimeric EEA1". Mol. Cell 8 (5): 947–58. November 2001. doi:10.1016/S1097-2765(01)00385-9. PMID 11741531.
- ↑ "FYVE fingers bind PtdIns(3)P". Nature 394 (6692): 432–3. July 1998. doi:10.1038/28767. PMID 9697764.
- ↑ "Membrane insertion of the FYVE domain is modulated by pH". Proteins 76 (4): 852–60. September 2009. doi:10.1002/prot.22392. PMID 19296456.
- ↑ "Signalling through phosphoinositide 3-kinases: the lipids take centre stage". Curr. Opin. Cell Biol. 11 (2): 219–25. April 1999. doi:10.1016/S0955-0674(99)80029-5. PMID 10209156.
- ↑ "Crystal structure of a phosphatidylinositol 3-phosphate-specific membrane-targeting motif, the FYVE domain of Vps27p". Cell 97 (5): 657–66. May 1999. doi:10.1016/S0092-8674(00)80776-X. PMID 10367894.
Further reading
- "Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger". J. Biol. Chem. 271 (39): 24048–54. September 1996. doi:10.1074/jbc.271.39.24048. PMID 8798641.
- "FYVE-finger proteins--effectors of an inositol lipid". J. Cell Sci. 112 (Pt 23): 4175–83. December 1999. PMID 10564636.
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