Biology:Homocitrate synthase
| homocitrate synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Homocitrate synthase homodimer, Schizosaccharomyces pombe | |||||||||
| Identifiers | |||||||||
| EC number | 2.3.3.14 | ||||||||
| CAS number | 9075-60-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a homocitrate synthase (EC 2.3.3.14) is an enzyme that catalyzes the chemical reaction
- acetyl-CoA + H2O + 2-oxoglutarate [math]\displaystyle{ \rightleftharpoons }[/math] (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
The 3 substrates of this enzyme are acetyl-CoA, H2O, and 2-oxoglutarate, whereas its two products are (R)-2-hydroxybutane-1,2,4-tricarboxylate and CoA.
This enzyme belongs to the family of transferases, specifically those acyltransferases that convert acyl groups into alkyl groups on transfer. The systematic name of this enzyme class is acetyl-CoA:2-oxoglutarate C-acetyltransferase (thioester-hydrolysing, carboxymethyl forming). Other names in common use include 2-hydroxybutane-1,2,4-tricarboxylate 2-oxoglutarate-lyase, (CoA-acetylating), acetyl-coenzyme A:2-ketoglutarate C-acetyl transferase, and homocitrate synthetase. This enzyme participates in lysine biosynthesis and pyruvate metabolism.
References
- "Enzymatic formation of homocitric acid, an intermediate in lysine biosynthesis". Biochem. Biophys. Res. Commun. 14 (3): 262–7. 1964. doi:10.1016/0006-291X(64)90446-2. PMID 5836514.
- H; Miyazaki, J; Kobashi, N; Nishiyama, M; Hoshino, T; Yamane, H (2002). "Characterization of bacterial homocitrate synthase involved in lysine biosynthesis". FEBS Lett. 522 (1–3): 35–40. doi:10.1016/S0014-5793(02)02877-6. PMID 12095615.
- "Kinetic mechanism of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae". Biochemistry 43 (37): 11790–5. 2004. doi:10.1021/bi048766p. PMID 15362863.
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