Biology:Metaphase

Short description: Stage of cell division

The mitotic spindle checkpoint verifies that all the chromosomes are aligned properly on the metaphase plate and prevents premature entry into anaphase.

Chromosomes lined up on the metaphase plate. Two views with the metaphase plate rotated 60°.

Stages of early mitosis in a vertebrate cell with micrographs of chromatids

In metaphase, microtubules from both duplicated centrosomes on opposite poles of the cell have completed attachment to kinetochores on condensed chromosomes. The centromeres of the chromosomes convene themselves on the metaphase plate, an imaginary line that is equidistant from the two spindle poles. This even alignment is due to the counterbalance of the pulling powers generated by the opposing kinetochore microtubules, analogous to a tug-of-war between two people of equal strength, ending with the destruction of B cyclin.

In order to prevent deleterious nondisjunction events, a key cell cycle checkpoint, the spindle checkpoint, verifies this evenly balanced alignment and ensures that every kinetochore is properly attached to a bundle of microtubules and is under balanced bipolar tension. Sister chromatids require active separase to hydrolyze the cohesin that bind them together prior to progression to anaphase. Any unattached or improperly attached kinetochores generate signals that prevent the activation of the anaphase promoting complex (cyclosome or APC/C), a ubiquitin ligase which targets securin and cyclin B for degradation via the proteosome. As long as securin and cyclin B remain active, separase remains inactive, preventing premature progression to anaphase.

Metaphase in cytogenetics and cancer studies

Human metaphase chromosomes (normal male karyotype)

The analysis of metaphase chromosomes is one of the main tools of classical cytogenetics and cancer studies. Chromosomes are condensed (thickened) and highly coiled in metaphase, which makes them most suitable for visual analysis. Metaphase chromosomes make the classical picture of chromosomes (karyotype). For classical cytogenetic analyses, cells are grown in short term culture and arrested in metaphase using mitotic inhibitor. Further they are used for slide preparation and banding (staining) of chromosomes to be visualised under microscope to study structure and number of chromosomes (karyotype). Staining of the slides, often with Giemsa (G banding) or Quinacrine, produces a pattern of in total up to several hundred bands. Normal metaphase spreads are used in methods like FISH and as a hybridization matrix for comparative genomic hybridization (CGH) experiments.

Malignant cells from solid tumors or leukemia samples can also be used for cytogenetic analysis to generate metaphase preparations. Inspection of the stained metaphase chromosomes allows the determination of numerical and structural changes in the tumor cell genome, for example, losses of chromosomal segments or translocations, which may lead to chimeric oncogenes, such as bcr-abl in chronic myelogenous leukemia.

External links

de:Mitose#Metaphase