Biology:Phthalate 4,5-dioxygenase

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phthalate 4,5-dioxygenase
EC number1.14.12.7
CAS number63626-44-8
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a phthalate 4,5-dioxygenase (EC is an enzyme that catalyzes the chemical reaction

phthalate + NADH + H+ + O2 [math]\displaystyle{ \rightleftharpoons }[/math] cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+

The 4 substrates of this enzyme are phthalate, NADH, H+, and O2, whereas its two products are cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate and NAD+.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor. The systematic name of this enzyme class is phthalate,NADH:oxygen oxidoreductase (4,5-hydroxylating). Other names in common use include PDO, and phthalate dioxygenase. This enzyme participates in 2,4-dichlorobenzoate degradation. It has 3 cofactors: iron, FMN, and Iron-sulfur.


  • "Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia". J. Biol. Chem. 262 (4): 1510–8. 1987. PMID 3805038.