Biology:Sarsasapogenin 3β-glucosyltransferase

From HandWiki
Short description: Class of enzymes
sarsasapogenin 3-β-glucosyltransferase
Identifiers
EC number2.4.1.193
CAS number117698-14-3
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a sarsasapogenin 3β-glucosyltransferase (EC 2.4.1.193) is an enzyme that catalyzes the chemical reaction

UDP-glucose + sarsasapogenin ⇌ UDP + sarsasapogenin 3-O-β-D-glucoside

Thus, the two substrates of this enzyme are UDP-glucose and sarsasapogenin [(3β,5β,25S)-spirostan-3-ol], whereas its two products are UDP and sarsasapogenin 3-O-β-D-glucoside.[1]

The enzyme was first isolated from the root of the common asparagus (Asparagus officinalis).[1] It is specific for substrate sterols with the uncommon 5β-configuration (sarsasapogenin and smilagenin), that is with a cis-linkage between the A and B rings of the steroid nucleus.[1]

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucose:(3β,5β,25S)-spirostan-3-ol 3-O-β-D-glucosyltransferase. This enzyme is also called uridine diphosphoglucose-sarsasapogenin glucosyltransferase.

References

  1. 1.0 1.1 1.2 "The occurrence of UDPG-dependent glucosyltransferase specific for sarsasapogenin in Asparagus officinalis", Phytochemistry 27 (9): 2743–47, 1988, doi:10.1016/0031-9422(88)80654-X