Biology:Proclavaminate amidinohydrolase
From HandWiki
Proclavaminate amidinohydrolase | |||||||||
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Identifiers | |||||||||
EC number | 3.5.3.22 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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In enzymology, a proclavaminate amidinohydrolase (EC 3.5.3.22) is an enzyme that catalyzes the chemical reaction
- amidinoproclavaminate + H2O [math]\displaystyle{ \rightleftharpoons }[/math] proclavaminate + urea
Thus, the two substrates of this enzyme are amidinoproclavaminate and H2O, whereas its two products are proclavaminate and urea.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is amidinoproclavaminate amidinohydrolase. Other names in common use include PAH, and proclavaminate amidino hydrolase. This enzyme participates in clavulanic acid biosynthesis.
References
- "Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction". Biochemistry 30 (8): 2281–92. 1991. doi:10.1021/bi00222a034. PMID 1998687.
- "Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities". J. Am. Chem. Soc. 123 (30): 7388–98. 2001. doi:10.1021/ja004025+. PMID 11472170.
- "New reactions in clavulanic acid biosynthesis". Curr. Opin. Chem. Biol. 6 (5): 583–9. 2002. doi:10.1016/S1367-5931(02)00392-7. PMID 12413541.
- "Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis". J. Bacteriol. 177 (13): 3714–20. 1995. PMID 7601835.
Original source: https://en.wikipedia.org/wiki/Proclavaminate amidinohydrolase.
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