Biology:Limulus clotting enzyme

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Limulus clotting enzyme
Identifiers
EC number3.4.21.86
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Limulus clotting enzyme (EC 3.4.21.86, clotting enzyme) is an enzyme.[1][2] This enzyme catalyses the following chemical reaction:

Selective cleavage of -Arg18- and -Arg47- bonds in coagulogen to form coagulin and fragments

This enzyme is present in the hemocyte granules of horseshoe crabs Limulus and Tachypleus. In the immunity-related clotting pathways of these organisms, it is the final enzyme responsible for the activation of coagulin.[3]

References

  1. "Proclotting enzyme from horseshoe crab hemocytes. cDNA cloning, disulfide locations, and subcellular localization". The Journal of Biological Chemistry 265 (36): 22426–33. December 1990. doi:10.1016/S0021-9258(18)45722-5. PMID 2266134. 
  2. "Further studies on lipopolysaccharide-sensitive serine protease zymogen (factor C): its isolation from Limulus polyphemus hemocytes and identification as an intracellular zymogen activated by alpha-chymotrypsin, not by trypsin". Journal of Biochemistry 109 (1): 150–7. January 1991. doi:10.1093/oxfordjournals.jbchem.a123337. PMID 2016264. 
  3. Iwanaga, S (May 2007). "Biochemical principle of Limulus test for detecting bacterial endotoxins.". Proceedings of the Japan Academy. Series B, Physical and Biological Sciences 83 (4): 110–9. doi:10.2183/pjab.83.110. PMID 24019589. Bibcode2007PJAB...83..110I. 

External links