Biology:N-acyl-D-amino-acid deacylase
From HandWiki
| N-acyl-D-amino-acid deacylase | |||||||||
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| Identifiers | |||||||||
| EC number | 3.5.1.81 | ||||||||
| CAS number | 65979-42-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a N-acyl-D-amino-acid deacylase (EC 3.5.1.81) is an enzyme that catalyzes the chemical reaction
- N-acyl-D-amino acid + H2O [math]\displaystyle{ \rightleftharpoons }[/math] an acid + D-amino acid
Thus, the two substrates of this enzyme are N-acyl-D-amino acid and H2O, whereas its two products are acid and D-amino acid.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acyl-D-amino acid amidohydrolase. It employs one cofactor, zinc.
Structural studies
As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1M7J, 1RJP, 1RJQ, 1RJR, 1RK5, 1RK6, 1V4Y, and 1V51.
References
- "Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli". Biosci. Biotechnol. Biochem. 59 (11): 2115–9. 1995. doi:10.1271/bbb.59.2115. PMID 8541651.
- "Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification". Protein Expr. Purif. 7 (4): 395–9. 1996. doi:10.1006/prep.1996.0059. PMID 8776758.
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