Physics:Spider silk

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Short description: Protein fiber made by spiders

A garden spider spinning its web
A female specimen of Argiope bruennichi wraps her prey in silk.
Indian Summer by Józef Chełmoński (1875, National Museum in Warsaw) depicts a peasant woman with a thread of gossamer in her hand.
Spider cocoon

Spider silk is a protein fibre spun by spiders. Spiders use their silk to make webs or other structures, which function as sticky nets to catch other animals, or as nests or cocoons to protect their offspring, or to wrap up prey. They can also use their silk to suspend themselves, to float through the air, or to glide away from predators. Most spiders vary the thickness and stickiness of their silk for different uses.

In some cases, spiders may even use silk as a source of food.[1] While methods have been developed to collect silk from a spider by force,[2] it is difficult to gather silk from many spiders compared to silk-spinning organisms such as silkworms.

All spiders produce silk, and even in non-web building spiders, silk is intimately tied to courtship and mating. Silk produced by females provides a transmission channel for male vibratory courtship signals, while webs and draglines provide a substrate for female sex pheromones. Observations of male spiders producing silk during sexual interactions are also common across phylogenetically widespread taxa. However, the function of male-produced silk in mating has received very little study.[3]



All spiders produce silks, and a single spider can produce up to seven different types of silk for different uses.[4] This is in contrast to insect silks, where an individual usually only produces one type of silk.[5] Spider silks may be used in many different ecological ways, each with properties to match the silk's function. As spiders have evolved, so has their silks' complexity and diverse uses, for example from primitive tube webs 300–400 million years ago to complex orb webs 110 million years ago.[6]

Use Example Reference
Prey capture The orb webs produced by the Araneidae (typical orb-weavers); tube webs; tangle webs; sheet webs; lace webs, dome webs; single thread used by the Bolas spiders for "fishing". [4][6]
Prey immobilisation Silk used as "swathing bands" to wrap up prey. Often combined with immobilising prey using a venom. In species of Scytodes the silk is combined with venom and squirted from the chelicerae. [4]
Reproduction Male spiders may produce sperm webs; spider eggs are covered in silk cocoons. [4][7]
Dispersal "Ballooning" or "kiting" used by smaller spiders to float through the air, for instance for dispersal. [8]
Source of food The kleptoparasitic Argyrodes eating the silk of host spider webs. Some daily weavers of temporary webs also eat their own unused silk daily, thus mitigating a heavy metabolic expense. [1][9]
Nest lining and nest construction Tube webs used by "primitive" spiders such as the European tube web spider (Segestria florentina). Threads radiate out of nest to provide a sensory link to the outside. Silk is a component of the lids of spiders that use "trapdoors", such as members of the family Ctenizidae, and the "water" or "diving bell" spider Argyroneta aquatica builds its diving bell of silk. [6]
Guide lines Some spiders that venture from shelter will leave a trail of silk by which to find their way home again. [9]
Drop lines and anchor lines Many spiders, such as the Salticidae, that venture from shelter and leave a trail of silk, use that as an emergency line in case of falling from inverted or vertical surfaces. Many others, even web dwellers, will deliberately drop from a web when alarmed, using a silken thread as a drop line by which they can return in due course. Some, such as species of Paramystaria, also will hang from a drop line when feeding. [9]
Alarm lines Some spiders that do not spin actual trap webs do lay out alarm webs that the feet of their prey (such as ants) can disturb, cueing the spider to rush out and secure the meal if it is small enough, or to avoid contact if the intruder seems too formidable. [9]
Pheromonal trails Some wandering spiders will leave a largely continuous trail of silk impregnated with pheromones that the opposite sex can follow to find a mate. [9]


right|thumb|A female Argiope picta immobilizing prey by wrapping a curtain of aciniform silk around the insect for later consumption Meeting the specification for all these ecological uses requires different types of silk suited to different broad properties, as either a fibre, a structure of fibres, or a silk-globule. These types include glues and fibres. Some types of fibres are used for structural support, others for constructing protective structures. Some can absorb energy effectively, whereas others transmit vibration efficiently. In a spider, these silk types are produced in different glands; so the silk from a particular gland can be linked to its use by the spider.

Gland Silk Use
Ampullate (major) Dragline silk – used for the web's outer rim and spokes, also for the lifeline and for ballooning.
Ampullate (minor) Used for temporary scaffolding during web construction.
Flagelliform Capture-spiral silk – used for the capturing lines of the web.
Tubuliform Egg cocoon silk – used for protective egg sacs.
Aciniform Used to wrap and secure freshly captured prey; used in the male sperm webs; used in stabilimenta.
Aggregate A silk glue of sticky globules.
Piriform Used to form bonds between separate threads for attachment points.


Mechanical properties

Each spider and each type of silk has a set of mechanical properties optimised for their biological function.

Most silks, in particular dragline silk, have exceptional mechanical properties. They exhibit a unique combination of high tensile strength and extensibility (ductility). This enables a silk fibre to absorb a large amount of energy before breaking (toughness, the area under a stress-strain curve).

An illustration of the differences between toughness, stiffness and strength

A frequent mistake made in the mainstream media is to confuse strength and toughness, when comparing silk to other materials. Weight for weight, silk is stronger than steel, but not as strong as Kevlar. Spider silk is, however, tougher than both.

The variability of mechanical properties of spider silk fibres may be important and it is related to their degree of molecular alignment.[10] Mechanical properties depend strongly on the ambient conditions, i.e. humidity and temperature.[11]


A dragline silk's tensile strength is comparable to that of high-grade alloy steel (450−2000 MPa),[12][13] and about half as strong as aramid filaments, such as Twaron or Kevlar (3000 MPa).[14]


Consisting of mainly protein, silks are about a sixth of the density of steel (1.3 g/cm3). As a result, a strand long enough to circle the Earth would weigh about 2 kilograms (4.4 lb). (Spider dragline silk has a tensile strength of roughly 1.3 GPa. The tensile strength listed for steel might be slightly higher – e.g. 1.65 GPa,[15][16] but spider silk is a much less dense material, so that a given weight of spider silk is five times as strong as the same weight of steel.)

Energy density

The energy density of dragline spider silk is roughly 1.2×108 J/m3.[17]


Silks are also extremely ductile, with some able to stretch up to five times their relaxed length without breaking.


The combination of strength and ductility gives dragline silks a very high toughness (or work to fracture), which "equals that of commercial polyaramid (aromatic nylon) filaments, which themselves are benchmarks of modern polymer fibre technology".[18][19]


While unlikely to be relevant in nature, dragline silks can hold their strength below -40 °C (-40 °F) and up to 220 °C (428 °F).[20] As occurs in many materials, spider silk fibres undergo a glass transition. The glass-transition temperature depends on the humidity, as water is a plasticiser for the silk.[11]


When exposed to water, dragline silks undergo supercontraction, shrinking up to 50% in length and behaving like a weak rubber under tension.[11] Many hypotheses have been suggested as to its use in nature, with the most popular being to automatically tension webs built in the night using the morning dew.


The toughest known spider silk is produced by the species Darwin's bark spider (Caerostris darwini): "The toughness of forcibly silked fibers averages 350 MJ/m3, with some samples reaching 520 MJ/m3. Thus, C. darwini silk is more than twice as tough as any previously described silk, and over 10 times tougher than Kevlar".[21]

Adhesive properties

Silk fibre is a two-compound pyriform secretion, spun into patterns (called "attachment discs") that are employed to adhere silk threads to various surfaces using a minimum of silk substrate.[22] The pyriform threads polymerise under ambient conditions, become functional immediately, and are usable indefinitely, remaining biodegradable, versatile and compatible with numerous other materials in the environment.[22] The adhesive and durability properties of the attachment disc are controlled by functions within the spinnerets.[23] Some adhesive properties of the silk resemble glue, consisting of microfibrils and lipid enclosures.[22]

Types of silk

Many species of spiders have different glands to produce silk with different properties for different purposes, including housing, web construction, defence, capturing and detaining prey, egg protection, and mobility (fine "gossamer" thread for ballooning, or for a strand allowing the spider to drop down as silk is extruded). Different specialised silks have evolved with properties suitable for different uses. For example, Argiope argentata has five different types of silk, each used for a different purpose:[24][25]

Silk Use
major-ampullate (dragline) silk Used for the web's outer rim and spokes and also for the lifeline. Can be as strong per unit weight as steel, but much tougher.
capture-spiral (flagelliform) silk Used for the capturing lines of the web. Sticky, extremely stretchy and tough. The capture spiral is sticky due to droplets of aggregate (a spider glue) that is placed on the spiral. The elasticity of flagelliform allows for enough time for the aggregate to adhere to the aerial prey flying into the web.
tubiliform (a.k.a. cylindriform) silk Used for protective egg sacs. Stiffest silk.
aciniform silk Used to wrap and secure freshly captured prey. Two to three times as tough as the other silks, including dragline.
minor-ampullate silk Used for temporary scaffolding during web construction.


Macroscopic structure down to protein hierarchy

Structure of spider silk. Inside a typical fibre there are crystalline regions separated by amorphous linkages. The crystals are beta-sheets that have assembled together.

Silks, like many other biomaterials, have a hierarchical structure. The primary structure is the amino acid sequence of its proteins (spidroin), mainly consisting of highly repetitive glycine and alanine blocks,[26][27] which is why silks are often referred to as a block co-polymer. On a secondary structure level, the short side chained alanine is mainly found in the crystalline domains (beta sheets) of the nanofibril, glycine is mostly found in the so-called amorphous matrix consisting of helical and beta turn structures.[27][28] It is the interplay between the hard crystalline segments, and the strained elastic semi-amorphous regions, that gives spider silk its extraordinary properties.[29][30] Various compounds other than protein are used to enhance the fibre's properties. Pyrrolidine has hygroscopic properties which keeps the silk moist while also warding off ant invasion. It occurs in especially high concentration in glue threads. Potassium hydrogen phosphate releases hydrogen ions in aqueous solution, resulting in a pH of about 4, making the silk acidic and thus protecting it from fungi and bacteria that would otherwise digest the protein. Potassium nitrate is believed to prevent the protein from denaturing in the acidic milieu.[31]

This first very basic model of silk was introduced by Termonia in 1994[32] who suggested crystallites embedded in an amorphous matrix interlinked with hydrogen bonds. This model has refined over the years: semi-crystalline regions were found[27] as well as a fibrillar skin core model suggested for spider silk,[33] later visualised by AFM and TEM.[34] Sizes of the nanofibrillar structure and the crystalline and semi-crystalline regions were revealed by neutron scattering.[35]

It has been possible to relate microstructural information and macroscopic mechanical properties of the fibres.[36] The results show that ordered regions (i) mainly reorient by deformation for low-stretched fibres and (ii) the fraction of ordered regions increases progressively for higher stretching of the fibres.

Biosynthesis and fibre spinning

The production of silks, including spider silk, differs in an important aspect from the production of most other fibrous biological materials: rather than being continuously grown as keratin in hair, cellulose in the cell walls of plants, or even the fibres formed from the compacted faecal matter of beetles;[17] it is "spun" on demand from liquid silk precursor out of specialised glands.[38]

The spinning process occurs when a fibre is pulled away from the body of a spider, whether by the spider's legs, by the spider's falling under its own weight, or by any other method including being pulled by humans. The term "spinning" is misleading because no rotation of any component occurs, but rather comes from analogy to the textile spinning wheels. Silk production is a pultrusion,[39] similar to extrusion, with the subtlety that the force is induced by pulling at the finished fibre rather than being squeezed out of a reservoir. The unspun silk fibre is pulled through silk glands of which there may be both numerous duplicates and different types of gland on any one spider species.[38]

Silk gland

Schematic of the spiders spinning apparatus and structural hierarchy in silk assembling related to assembly into fibers.[40][41][42][43][44] In the process of dragline production, the primary structure protein is secreted first from secretory granules in the tail.[45] In the ampullate (neutral environment, pH = 7), the proteins form a soft micelle of several tens of nanometers by self-organization because the hydrophilic terminals are excluded.[46] In ampullate, the concentration of the protein is very high.[47][48] Then, the micelles are squeezed into the duct. The long axis direction of the molecules is aligned parallel to the duct by a mechanical frictional force and partially oriented.[45][46][49] The continuous lowering of pH from 7.5 to 8.0 in the tail to presumably close to 5.0 occurs at the end of the duct.[41][50][51] Ion exchange, acidification, and water removal all happen in the duct.[42] The shear and elongational forces lead to phase separation.[42] In the acidic bath of the duct, the molecules attain a high concentration liquid crystal state.[52] Finally, the silk is spun from the taper exterior. The molecules become more stable helixes and β-sheets from the liquid crystal.

The gland's visible, or external, part is termed the spinneret. Depending on the complexity of the species, spiders will have two to eight spinnerets, usually in pairs. There exist highly different specialised glands in different spiders, ranging from simply a sac with an opening at one end, to the complex, multiple-section major ampullate glands of the golden silk orb-weavers.[53]

Behind each spinneret visible on the surface of the spider lies a gland, a generalised form of which is shown in the figure to the right, "Schematic of a generalised gland".

Schematic of a generalised gland of a Golden silk orb-weaver. Each differently coloured section highlights a discrete section of the gland.[54][55]
Gland characteristics
  1. The first section of the gland labelled 1 on Figure 1 is the secretory or tail section of the gland. The walls of this section are lined with cells that secrete proteins Spidroin I and Spidroin II, the main components of this spider's dragline. These proteins are found in the form of droplets that gradually elongate to form long channels along the length of the final fibre, hypothesised to assist in preventing crack formation or even self-healing of the fibre.[56]
  2. The second section is the storage sac. This stores and maintains the gel-like unspun silk dope until it is required by the spider. In addition to storing the unspun silk gel, it secretes proteins that coat the surface of the final fibre.[18]
  3. The funnel rapidly reduces the large diameter of the storage sac to the small diameter of the tapering duct.
  4. The final length is the tapering duct, the site of most of the fibre formation. This consists of a tapering tube with several tight about turns, a valve almost at the end (mentioned in detail at point No. 5 below) ending in a spigot from which the solid silk fibre emerges. The tube here tapers hyperbolically, therefore the unspun silk is under constant elongational shear stress, which is an important factor in fibre formation. This section of the duct is lined with cells that exchange ions, reduce the dope pH from neutral to acidic, and remove water from the fibre.[57] Collectively, the shear stress and the ion and pH changes induce the liquid silk dope to undergo a phase transition and condense into a solid protein fibre with high molecular organisation. The spigot at the end has lips that clamp around the fibre, controlling fibre diameter and further retaining water.
  5. Almost at the end of the tapering duct is a valve, approximate position marked "5" on figure 1. Though discovered some time ago, the precise purpose of this valve is still under discussion. It is believed to assist in restarting and rejoining broken fibres,[58] acting much in the way of a helical pump, regulating the thickness of the fibre,[39] and/or clamping the fibre as a spider falls upon it.[58][59] There is some discussion of the similarity of the silk worm's silk press and the roles each of these valves play in the production of silk in these two organisms.

Throughout the process the unspun silk appears to have a nematic texture,[60] in a similar manner to a liquid crystal, arising in part due to the extremely high protein concentration of silk dope (around 30% in terms of weight per volume).[61] This allows the unspun silk to flow through the duct as a liquid but maintain a molecular order.

As an example of a complex spinning field, the spinneret apparatus of an adult Araneus diadematus (garden cross spider) consists of the glands shown below.[31] Similar multiple gland architecture exists in the black widow spider.[62]

  • 500 pyriform glands for attachment points
  • 4 ampullate glands for the web frame
  • about 300 aciniform glands for the outer lining of egg sacs, and for ensnaring prey
  • 4 tubuliform glands for egg sac silk
  • 4 aggregate glands for adhesive functions
  • 2 coronate glands for the thread of adhesion lines

Artificial synthesis

Single strand of artificial spider silk produced under laboratory conditions

To artificially synthesise spider silk into fibres, there are two broad areas that must be covered. These are synthesis of the feedstock (the unspun silk dope in spiders), and synthesis of the spinning conditions (the funnel, valve, tapering duct, and spigot). There have been a number of different approaches but few of these methods have produced silk that can efficiently be synthesised into fibres.


The molecular structure of unspun silk is both complex and extremely long. Though this endows the silk fibres with their desirable properties, it also makes replication of the fibre somewhat of a challenge. Various organisms have been used as a basis for attempts to replicate some components or all of some or all of the proteins involved. These proteins must then be extracted, purified and then spun before their properties can be tested.

Organism Details Average Maximum breaking stress (MPa) Average Strain (%) Reference
Darwin's bark spider (Caerostris darwini) Malagasy spider famed for making webs with strands up to 25 m long, across rivers. "C. darwini silk is more than twice as tough as any previously described silk" 1850 ±350 33 ±0.08 [21]
Nephila clavipes Typical golden orb weaving spider 710–1200 18–27 [63][64]
Bombyx mori Silkworms Silkworms were genetically altered to express spider proteins and fibres measured.[65] 660 18.5 [66]
E. coli Synthesising a large and repetitive molecule (~300 kDa) is complex, but required for the strongest silk. Here E. coli was engineered to produce a 556 kDa protein. Fibers spun from these synthetic spidroins are the first to fully replicate the mechanical performance of natural spider silk by all common metrics. 1030 ±110 18 ±6 [67]
Goats Goats were genetically modified to secrete silk proteins in their milk, which could then be purified. 285–250 30–40 [68]
Tobacco & potato plants Tobacco and potato plants were genetically modified to produce silk proteins. Patents were granted,[69] but no fibres have yet been described in the literature. n/a n/a [70]


Spider silks with comparatively simple molecular structure need complex ducts to be able to spin an effective fibre. There have been a number of methods used to produce fibres, of which the main types are briefly discussed below.

Syringe and needle

Feedstock is simply forced through a hollow needle using a syringe. This method has been shown to make fibres successfully on multiple occasions.[71][72]

Although very cheap and easy to produce, the shape and conditions of the gland are very loosely approximated. Fibres created using this method may need encouragement to change from liquid to solid by removing the water from the fibre with such chemicals as the environmentally undesirable methanol[73] or acetone,[72] and also may require post-stretching of the fibre to attain fibres with desirable properties.[74][71]

Small silk sheet and silk nanowire synthesis on superhydrophobic surfaces

Placing a solution of spider silk on a superhydrophobic surface can generate sheets, particles and nanowires of spider silk. [75] [76]

Large silk sheets

Self-assembly of silk at standing liquid-gas interphases of a spider silk solution allows forming tough and strong sheets. These sheets are now explored for mimicking of the basal membrane in tissue modeling. [77] [78]


As the field of microfluidics matures, it is likely that more attempts to spin fibres will be made using microfluidics. These have the advantage of being very controllable and able to test spin very small volumes of unspun fibre[79][80] but setup and development costs are likely to be high. A patent has been granted in this area for spinning fibres in a method mimicking the process found in nature, and fibres are successfully being continuously spun by a commercial company.[81]


Electrospinning is a very old technique whereby a fluid is held in a container in a manner such that it is able to flow out through capillary action. A conducting substrate is positioned below, and a large difference in electrical potential is applied between the fluid and the substrate. The fluid is attracted to the substrate, and tiny fibres jump almost instantly from their point of emission, the Taylor cone, to the substrate, drying as they travel. This method has been shown to create nano-scale fibres from both silk dissected from organisms and regenerated silk fibroin.

Other artificial shapes formed from silk

Silk can be formed into other shapes and sizes such as spherical capsules for drug delivery, cell scaffolds and wound healing, textiles, cosmetics, coatings, and many others.[82][83] Spider silk proteins can also self-assemble on superhydrophobic surfaces to generate nanowires, as well as micron-sized circular sheets.[83] It has recently been shown that recombinant spider silk proteins can self-assemble at the liquid air interface of a standing solution to form protein permeable, strong, and flexible nanomembranes that support cell proliferation. Suggested applications include skin transplants, and supportive membranes in organ-on-a-chip.[84] These spider silk nanomembranes have also been used to create a static in-vitro model of a blood vessel.[85]

Research milestones

Due to spider silk being a scientific research field with a long and rich history, there can be unfortunate occurrences of researchers independently rediscovering previously published findings. What follows is a table of the discoveries made in each of the constituent areas, acknowledged by the scientific community as being relevant and significant by using the metric of scientific acceptance, citations. Thus, only papers with 50 or more citations are included.

Table of significant papers (50 or more citations)
Area of contribution Year Main researchers [Ref] Title of paper Contribution to the field
Chemical Basis 1960 Fischer, F. & Brander, J.[86] "Eine Analyse der Gespinste der Kreuzspinne" (Amino acid composition analysis of spider silk)
1960 Lucas, F. et al.[87][88] "The Composition of Arthropod Silk Fibroins; Comparative studies of fibroins"
Gene Sequence 1990 Xu, M. & Lewis, R. V.[89] "Structure of a Protein Superfiber − Spider Dragline Silk"
Mechanical Properties 1964 Lucas, F.[90] "Spiders and their silks" First time compared mechanical properties of spider silk with other materials in a scientific paper.
1989 Vollrath, F. & Edmonds, D. T.[91] "Modulation of the Mechanical Properties of Spider Silk by Coating with Water" First important paper suggesting the water interplay with spider silk fibroin modulating the properties of silk.
2001 Vollrath, F. & Shao, Z.Z.[92] "The effect of spinning conditions on the mechanics of a spider's dragline silk"
2006 Plaza, G.R., Guinea, G.V., Pérez-Rigueiro, J. & Elices, M.[11] "Thermo-hygro-mechanical behavior of spider dragline silk: Glassy and rubbery states" Combined effect of humidity and temperature on the mechanical properties. Glass-transition temperature dependence on humidity.
Structural Characterisation 1992 Hinman, M.B. & Lewis, R. V[26] "Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber"
1994 Simmons, A. et al.[93] "Solid-State C-13 Nmr of Nephila-Clavipes Dragline Silk Establishes Structure and Identity of Crystalline Regions" First NMR study of spider silk.
1999 Shao, Z., Vollrath, F. et al.[94] "Analysis of spider silk in native and supercontracted states using Raman spectroscopy" First Raman study of spider silk.
1999 Riekel, C., Muller, al.[95] "Aspects of X-ray diffraction on single spider fibers" First X-ray on single spider silk fibres.
2000 Knight, D.P., Vollrath, F. et al.[96] "Beta transition and stress-induced phase separation in the spinning of spider dragline silk" Secondary structural transition confirmation during spinning.
2001 Riekel, C. & Vollrath, F.[97] "Spider silk fibre extrusion: combined wide- and small-angle X- ray microdiffraction experiments" First X-ray on spider silk dope.
2002 Van Beek, J. D. et al.[28] "The molecular structure of spider dragline silk: Folding and orientation of the protein backbone"
Structure-Property Relationship
1994 Termonia, Y[32] "Molecular Modeling of Spider Silk Elasticity" X-ray evidence presented in this paper; simple model of crystallites embedded in amorphous regions.
1996 Simmons, A. et al.[27] "Molecular orientation and two-component nature of the crystalline fraction of spider dragline silk" Two types of alanine-rich crystalline regions were defined.
2006 Vollrath, F. & Porter, D.[98] "Spider silk as an archetypal protein elastomer" New insight and model to spider silk based on Group Interaction Modelling.
Native Spinning 1991 Kerkam, K., Kaplan, D. et al.[99] "Liquid Crystallinity of Natural Silk Secretions"
1999 Knight, D.P. & Vollrath, F.[100] "Liquid crystals and flow elongation in a spider's silk production line"
2001 Vollrath, F. & Knight, D.P.[17] "Liquid crystalline spinning of spider silk" The most cited paper on spider silk
2005 Guinea, G.V., Elices, M., Pérez-Rigueiro, J. & Plaza, G.R.[10] "Stretching of supercontracted fibers: a link between spinning and the variability of spider silk" Explanation of the variability of mechanical properties.
Reconstituted /Synthetic Spider Silk and Artificial Spinning 1995 Prince, J. T., Kaplan, D. L. et al.[101] "Construction, Cloning, and Expression of Synthetic Genes Encoding Spider Dragline Silk" First successful synthesis of Spider silk by E. coli.
1998 Arcidiacono, S., Kaplan, D.L. et al.[102] "Purification and characterization of recombinant spider silk expressed in Escherichia coli"
1998 Seidel, A., Jelinski, L.W. et al.[103] "Artificial Spinning of Spider Silk" First controlled wet-spinning of reconstituted spider silk.

Human uses

A cape made from Madagascar golden orb spider silk[104]

Peasants in the southern Carpathian Mountains used to cut up tubes built by Atypus and cover wounds with the inner lining. It reportedly facilitated healing, and even connected with the skin. This is believed to be due to antiseptic properties of spider silk[105] and because the silk is rich in vitamin K, which can be effective in clotting blood.[106] Due to the difficulties in extracting and processing substantial amounts of spider silk, the largest known piece of cloth made of spider silk is an 11-by-4-foot (3.4 by 1.2 m) textile with a golden tint made in Madagascar in 2009.[107] Eighty-two people worked for four years to collect over one million golden orb spiders and extract silk from them.[108]

The silk of Nephila clavipes was used in research concerning mammalian neuronal regeneration.[109]

Spider silk has been used as a thread for crosshairs in optical instruments such as telescopes, microscopes,[110] and telescopic rifle sights.[111] In 2011, spider silk fibres were used in the field of optics to generate very fine diffraction patterns over N-slit interferometric signals used in optical communications.[112] In 2012, spider silk fibres were used to create a set of violin strings.[113]

Development of methods to mass-produce spider silk has led to manufacturing of military, medical and consumer goods, such as ballistics armour, athletic footwear, personal care products, breast implant and catheter coatings, mechanical insulin pumps, fashion clothing, and outerwear.[114]

Spider silk is used to suspend inertial confinement fusion targets during laser ignition, as it remains considerably elastic and has a high energy to break at temperatures as low as 10–20 K. In addition, it is made from "light" atomic number elements that won't emit x-rays during irradiation that could preheat the target so that the pressure differential required for fusion is not achieved.[115]

Spider silk has been used to create biolenses that could be used in conjunction with lasers to create high-resolution images of the inside of the human body.[116]

Attempts at producing synthetic spider silk

Proposed framework for producing artificial skin from spider silk to help patients with burns.

Replicating the complex conditions required to produce fibres that are comparable to spider silk has proven difficult in research and early-stage manufacturing. Through genetic engineering, Escherichia coli bacteria, yeasts, plants, silkworms, and animals other than silkworms have been used to produce spider silk proteins, which have different, simpler characteristics than those from a spider.[114] Extrusion of protein fibres in an aqueous environment is known as "wet-spinning". This process has so far produced silk fibres of diameters ranging from 10 to 60 μm, compared to diameters of 2.5–4 μm for natural spider silk. Artificial spider silks have fewer and simpler proteins than natural dragline silk, and are consequently half the diameter, strength, and flexibility of natural dragline silk.[114]

  • In March 2010, researchers from the Korea Advanced Institute of Science & Technology succeeded in making spider silk directly using the bacteria E. coli, modified with certain genes of the spider Nephila clavipes. This approach eliminates the need to milk spiders and allows the manufacture of the spider silk in a more cost-effective manner.[117]
  • A 556 kDa spider silk protein was manufactured from 192 repeat motifs of the Nephila clavipes dragline spidroin, having similar mechanical characteristics as their natural counterparts, i.e., tensile strength (1.03 ± 0.11 GPa), modulus (13.7 ± 3.0 GPa), extensibility (18 ± 6%), and toughness (114 ± 51 MJ/m3).[67]
  • The company AMSilk developed spidroin using bacteria, making it into an artificial spider silk.[114][118]
  • The company Bolt Threads produces a recombinant spidroin using yeast, for use in apparel fibers and personal care. They produced the first commercial apparel products made of recombinant spider silk, trademarked Microsilk, demonstrated in ties and beanies. They have also partnered with vegan activist and luxury designer Stella McCartney as well as Adidas to produce Microsilk garments.[119][120]
  • The company Kraig Biocraft Laboratories used research from the Universities of Wyoming and Notre Dame to create silkworms that were genetically altered to produce spider silk.[121][122]
  • The now defunct Canadian biotechnology company Nexia successfully produced spider silk protein in transgenic goats that carried the gene for it; the milk produced by the goats contained significant quantities of the protein, 1–2 grams of silk proteins per litre of milk. Attempts to spin the protein into a fibre similar to natural spider silk resulted in fibres with tenacities of 2–3 grams per denier.[123] Nexia used wet spinning and squeezed the silk protein solution through small extrusion holes in order to simulate the behavior of the spinneret, but this procedure was not sufficient to replicate the stronger properties of native spider silk.[124]
  • The company Spiber has produced a synthetic spider silk that they are calling Q/QMONOS. In partnership with Goldwin, a ski parka made from this synthetic spider silk is currently in testing and is to be in mass production soon for less than $120,000 YEN.[125][126]


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